A part of MilliporeSigma

ABE1870 | Anti-AHCYL1 (IRBIT) Antibody

100 µL  
Retrieving price...
Price could not be retrieved
Minimum Quantity needs to be mulitiple of
Upon Order Completion More Information
You Saved ()
Request Pricing
Limited AvailabilityLimited Availability
Limited Quantities Available
    Remaining : Will advise
      Remaining : Will advise
      Will advise
      Contact Customer Service

      Special Offers


      Contact Customer Service

      Click To Print This Page


      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H WB Ch Affinity Purified Polyclonal Antibody
      Catalogue NumberABE1870
      DescriptionAnti-AHCYL1 (IRBIT) Antibody
      Alternate Names
      • Putative adenosylhomocysteinase 2
      • AHCYL1(IRBIT)
      • AdoHcyase 2
      • DC-expressed AHCY-like molecule
      • IP(3)Rs binding protein released with IP(3)
      • IRBIT
      • S-adenosyl-L-homocysteine hydrolase 2
      • S-adenosylhomocysteine hydrolase-like protein 1
      Background InformationPutative adenosylhomocysteinase 2 (EC; UniProt O43865; also known as AdoHcyase 2, DC-expressed AHCY-like molecule, IP(3)Rs binding protein released with IP(3), IRBIT, S-adenosyl-L-homocysteine hydrolase 2, S-adenosylhomocysteine hydrolase-like protein 1) is encoded by the AHCYL1 (also known as DCAL, PPP1R78, PRO0233, XPVKONA) gene (Gene ID 10768) in human. Originally identified by its binding and antagonist activity against teh intracellular calcium channel, IP3R, IRBIT is now known to interact with multiple targets and regulate diverse physiological events in addition to blocking IP3 access to IP3R. IRBIT complexes with ribonucleotide reductase (RNR) in a dATP-dependent manner and stabilizes RNR in its inactive, dATP-bound state. IRBIT also targets CaMKIIα kinase for inhibition by blocking calmodulin binding. Other known IRBIT targets include the ion transporter NBCe1-B, the Na+/H+ exchanger NHE3, the Cl- channel CFTR, the Cl−/HCO3− exchanger Slc26a6, the Fip1 component of the cleavage and polyadenylation specificity factor (CPSF) complex. IRBIT contains an S-adenosyl-l-homocysteine hydrolase (AHCY) homology domain in and an N-terminal extension containing a serine-rich region with multiple phosphorylation sites. Long-IRBIT, but not IRBIT, has an additional N-terminal appendage, termed the LISN (Long-IRBIT specific N-terminal) domain. IRBIT is highly conserved among species, and the human and mouse orthologs have 100% protein identity.
      Product Information
      FormatAffinity Purified
      PresentationPurified chicken polyclonal antibody in PBS with 0.02% Sodium azide.
      Key Applications
      • Western Blotting
      Application NotesWestern Blotting Analysis: A representative lot detected Tet-inducible short hairpin RNA-mediated AHCYL1 (IRBIT) downregulation in HeLa cells (Arnaoutov, A., and Dasso, M. (2014). Science 345(6203):1512-1515).
      Western Blotting Analysis: A representative lot detected immunoprecipitated AHCYL1 (IRBIT) from HeLa lysates by Western blotting (Arnaoutov, A., and Dasso, M. (2014). Science 345(6203):1512-1515).
      Western Blotting Analysis: A representative lot detected the association of His-tagged human AHCYL1 (IRBIT) with human IP3R1(1-604) GST fusion protein by Western blotting analysis of the affinity pull-down obtained with Glutathione beads (Arnaoutov, A., and Dasso, M. (2014). Science 345(6203):1512-1515).
      Biological Information
      ImmunogenRecombinant protein corresponding to human AHCYL1(IRBIT).
      ConcentrationPlease refer to lot specific datasheet.
      SpecificityExpected to react with both spliced isoforms reported by UniProt (O43865-1 and O43865-2).
      Species Reactivity
      • Human
      Species Reactivity NoteHuman. Predicted to react with Mouse based on 100% sequence homology.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • AHCYL1
      • DCAL
      • XPVKONA
      Purification MethodAffinity Purfied
      UniProt Number
      Molecular Weight~70 kDa observed. 59.0 kDa (isoform 1) and 53.8 kDa (isoform 2) calculated. Uncharacterized band(s) may appear in some lysates.
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in HeLa cell lysate.

      Western Blotting Analysis: A 1:500 dilution of this antibody detected AHCYL1 (IRBIT) in 10 µg of HeLa cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information


      Certificates of Analysis

      TitleLot Number
      Anti-AHCYL1(IRBIT) -Q2635129 Q2635129


      Reference overviewPub Med ID
      Enzyme regulation. IRBIT is a novel regulator of ribonucleotide reductase in higher eukaryotes.
      Arnaoutov, A; Dasso, M
      Science (New York, N.Y.) 345 1512-5 2014

      Show Abstract
      25237103 25237103