ST1673 Anti-AFP Mouse mAb (1G7)

ST1673
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityHostAntibody Type
      H M Monoclonal Antibody

      Pricing & Availability

      Catalog Number AvailabilityPackaging Qty/Pack Price Quantity
      ST1673-100UG
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          Glass bottle 100 μg
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          Description
          OverviewRecognizes the ~70-75 kDa AFP protein in HepG2 cells.
          Catalogue NumberST1673
          Brand Family Calbiochem®
          SynonymsAnti-α-Fetoprotein
          References
          Product Information
          FormLiquid
          FormulationIn PBS, pH 7.2.
          Negative control293T cells
          Positive controlHepG2 cells
          PreservativeNone
          Applications
          Key Applications Enzyme-Linked Immunosorbent Assay
          Immunoblotting (Western Blotting)
          Immunocytochemistry
          Application NotesELISA (see coments)
          Immunoblotting (1-5 µg/ml)
          Immunocytochemistry (10 µg/ml)
          Application CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
          Biological Information
          ImmunogenA recombinant polypeptide corresponding to amino acids of 500-610 human AFP, expressed as a GST fusion protein
          Clone1G7
          HostMouse
          IsotypeIgG
          Species Reactivity
          • Human
          Antibody TypeMonoclonal Antibody
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Blue Ice Only
          Toxicity Regulatory Review
          Storage -20°C
          Avoid freeze/thaw Avoid freeze/thaw
          Do not freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          Anti-AFP Mouse mAb (1G7) SDS

          Title

          Safety Data Sheet (SDS) 

          Anti-AFP Mouse mAb (1G7) Certificates of Analysis

          TitleLot Number
          ST1673
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision10-November-2010 RFH
          SynonymsAnti-α-Fetoprotein
          ApplicationELISA (see coments)
          Immunoblotting (1-5 µg/ml)
          Immunocytochemistry (10 µg/ml)
          DescriptionPurified mouse monoclonal antibody. Recognizes the ~70-75 kDa AFP protein.
          BackgroundThis gene encodes α-fetoprotein, a major plasma protein produced by the yolk sac and the liver during fetal life. α-fetoprotein expression in adults is often associated with hepatoma or teratoma. However, hereditary persistence of α-fetoprotein may also be found in individuals with no obvious pathology. The protein is thought to be the fetal counterpart of serum albumin, and the α-fetoprotein and albumin genes are present in tandem in the same transcriptional orientation on chromosome 4. α-fetoprotein is found in monomeric as well as dimeric and trimeric forms, and binds copper, nickel, fatty acids and bilirubin. The level of α-fetoprotein in amniotic fluid is used to measure renal loss of protein to screen for spina bifida and anencephaly.
          HostMouse
          ImmunogenA recombinant polypeptide corresponding to amino acids of 500-610 human AFP, expressed as a GST fusion protein
          Clone1G7
          IsotypeIgG
          Specieshuman
          Positive controlHepG2 cells
          Negative control293T cells
          FormLiquid
          FormulationIn PBS, pH 7.2.
          PreservativeNone
          CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
          Storage -20°C
          Avoid freeze/thaw
          Do Not Freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
          Toxicity Regulatory Review