MABN32 | Anti-ADAM 22 Antibody, clone N46/30

MABN32
100 µg  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      R, M IHC, IP, IF M Purified Monoclonal Antibody
      Description
      Catalogue NumberMABN32
      DescriptionAnti-ADAM 22 Antibody, clone N46/30
      Alternate Names
      • ADAM metallopeptidase domain 22
      • Metalloproteinase-disintegrin ADAM22-3
      • a disintegrin and metalloproteinase domain 22
      • disintegrin and metalloproteinase domain-containing protein 22
      • Metalloproteinase-like, disintegrin-like, and cysteine-rich protein 2
      Background InformationADAM 22 belongs to the ADAM gene family which have been shown to bind integrin and therefore may have a part in cell to cell or cell to matrix interactions. ADAM 22 is unique in the fact that it is only observed in the nervous system and predominantly in the brain. ADAM 22 is attached by cytoskeletal scaffolds to the postsynaptic density and is a receptor for LGI1.
      References
      Product Information
      FormatPurified
      Control
      • Rat myelinated neurite tissue
      PresentationPurified mouse monoclonal IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Applications
      ApplicationAnti-ADAM 22 Antibody, clone N46/30 is an antibody against ADAM 22 for use in IH, IP & IF.
      Key Applications
      • Immunohistochemistry
      • Immunoprecipitation
      • Immunofluorescence
      Application NotesImmunohistochemistry Analysis: 1:400 dilution of this antibody detected ADAM 22 in rat neurons/myelin in putamen area.

      Western Blot Analysis: A previous lot of this antibody was used by an independent laboratory to detect ADAM 22 in rat and mouse brain tissue lysate (James Trimmer, UC Davis/NIH NeuroMab Facility).

      Immunoprecipitation Analysis: A previous lot of this antibody was used by an independent laboratory in IP. (Ogawa, Y., et al. (2010). J Neurosci. 30(3):1038–1048.)

      Immunofluorescence Analysis: A previous lot of this antibody was used by an independent laboratory in IF. (Ogawa, Y., et al. (2010). J Neurosci. 30(3):1038–1048.)
      Biological Information
      ImmunogenRecombinant protein corresponding to mouse ADAM 22 at the cytoplasmic domain.
      EpitopeCytoplasmic domain
      CloneN46/30
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityThis antibody recognizes ADAM 22 at the cytoplasmic domain.
      IsotypeIgG2bκ
      Species Reactivity
      • Rat
      • Mouse
      Species Reactivity NoteDemonstrated to react with rat. Predicted to react with mouse based on immunogen design.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • MDC2
      • ADAM 22
      • ADAM22
      Purification MethodProtein G Purified
      UniProt Number
      UniProt SummaryFUNCTION: Probable ligand for integrin in the brain. This is a non catalytic metalloprotease-like protein. Involved in regulation of cell adhesion and spreading and in inhibition of cell proliferation (By similarity). Neuronal receptor for LGI1.

      SUBUNIT STRUCTURE: Interacts with LGI1. Can bind to LGI4.

      SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.

      TISSUE SPECIFICTY: Expressed at high levels in the brain. Strongly expressed in cerebellar granule cells and hippocampus. In spinal cord, expression is restricted to gray matter.

      PTM: The precursor is cleaved by a furin endopeptidase (By similarity).

      DISRUPTION PHENOTYPE: Mice display severe ataxia within one week after birth and die before weaning, probably due to convulsions. They display marked hypomyelination of the peripheral nerves.

      SEQUENCE SIMILARITIES: Contains 1 disintegrin domain.

      Contains 1 EGF-like domain.

      Contains 1 peptidase M12B domain.
      Molecular WeightCalculated Mw is ~100 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Immunohistochemistry in rat myelinated neurite tissue.

      Immunohistochemistry Analysis: 1:400 dilution of this antibody detected ADAM 22 in rat myelinated neurite tissue.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications