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AB19036 | Anti-ADAM 15 Antibody, CT

100 µg  
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      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H WB Rb Affinity Purified Polyclonal Antibody
      Catalogue NumberAB19036
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-ADAM 15 Antibody, CT
      Alternate Names
      • Metargidin
      • MDC15
      Product Information
      FormatAffinity Purified
      PresentationAffinity purified immunoglobulin. Liquid in PBS containing 50% glycerol and 0.05% sodium azide
      ApplicationThis Anti-ADAM 15 Antibody, C-terminus is validated for use in WB for the detection of ADAM 15.
      Key Applications
      • Western Blotting
      Application NotesWestern blot: 1:500 Optimal working dilutions must be determined by end user.
      Biological Information
      ImmunogenA synthetic peptide derived from the C-terminal end of the cytoplasmic domain of human ADAM15
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      SpecificityThe ADAM (A Disintegrin and Metalloprotease) family of cell surface proteases mediate a number of cellular processes, including differentiation, cell fusion, cell-cell interactions, and shedding of plasma membrane-bound growth factor precursors1. Each ADAM contains a prodomain, a catalytic metalloprotease domain, a disintegrin domain, a transmembrane domain, and a cytoplasmic domain1. The cytoplasmic domain of ADAM15 is phosphorylated and interacts with Src family tyrosine kinases2. ADAM15 contains an RGD sequence within its disintegrin domain, and thus has been given the trivial name metargidin. In HUVEC, ADAM15 localizes to adherens junctions3. Although the mature ADAM15 has a molecular weight of 100 kD, ADAM family members can undergo proteolytic processing autocatalytically or by furin proteases to yield smaller molecular weight forms. This antibody recognizes the 100 kD full-length form in HUVEC lysates, but other forms are possible in different cell and tissue types.
      Species Reactivity
      • Human
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThe protein encoded by this gene is a member of the ADAM (a disintegrin and metalloproteinase) protein family. ADAM family members are type I transmembrane glycoproteins known to be involved in cell adhesion and proteolytic ectodomain processing of cytokines and adhesion molecules. This protein contains multiple functional domains including a zinc-binding metalloprotease domain, a disintegrin-like domain, as well as a EGF-like domain. Through its disintegrin-like domain, this protein specifically interacts with the integrin beta chain, beta 3. It also interacts with Src family protein-tyrosine kinases in a phosphorylation-dependent manner, suggesting that this protein may function in cell-cell adhesion as well as in cellular signaling. Multiple alternatively spliced transcript variants encoding distinct isoforms have been observed.
      Gene Symbol
      • ADAM15
      • MDC-15
      • metargidin
      • Metargidin
      • MDC15
      • EC 3.4.24.-
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: Q13444 # May be involved in cell-surface proteolysis, cell adhesion or intracellular protein maturation.
      COFACTOR: Binds 1 zinc ion per subunit (By similarity).
      SIZE: 814 amino acids; 87717 Da
      SUBUNIT: Interacts with ITAGV-ITGB3 (vitronectin receptor), PACSIN3 and SNX9. PACSIN3 and SNX9 preferentially bind the precursor but not the processed form of ADAM15, suggesting that the interaction occurs in a secretory pathway compartment prior to the medial Golgi (By similarity). Interacts specifically with Src family protein-tyrosine kinases (PTKs). Interacts with SH3PXD2A.
      SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.
      TISSUE SPECIFICITY: Ubiquitously expressed. Overexpressed in atherosclerotic lesions. Constitutively expressed in cultured endothelium and smooth muscle. Expressed in chondrocytes.
      DOMAIN: SwissProt: Q13444 The cytoplasmic domain interacts with endophilin I and sorting nexin 9 (By similarity). & Desintegrin domain binds to integrin alphaV-beta3. & The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
      PTM: The precursor is cleaved by a furin endopeptidase (By similarity). & Phosphorylation increases association with PTKs.
      SIMILARITY: Contains 1 disintegrin domain. & Contains 1 EGF-like domain. & Contains 1 peptidase M12B domain.
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain frozen at -20°C for up to 12 months.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information




      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number


      Reference overviewApplicationSpeciesPub Med ID
      The ADAM15 ectodomain is shed from secretory exosomes.
      Lee, HD; Kim, YH; Koo, BH; Kim, DS
      BMB reports 48 277-82 2015

      Show Abstract
      25208722 25208722
      miR-1247 functions by targeting cartilage transcription factor SOX9.
      Martinez-Sanchez, A; Murphy, CL
      The Journal of biological chemistry 288 30802-14 2013

      Show Abstract
      Western Blotting24014021 24014021
      Exosome release of ADAM15 and the functional implications of human macrophage-derived ADAM15 exosomes.
      Lee, HD; Koo, BH; Kim, YH; Jeon, OH; Kim, DS
      FASEB journal : official publication of the Federation of American Societies for Experimental Biology 26 3084-95 2012

      Show Abstract
      22505472 22505472
      EGFR and ADAMs cooperate to regulate shedding and endocytic trafficking of the desmosomal cadherin desmoglein 2.
      Klessner, JL; Desai, BV; Amargo, EV; Getsios, S; Green, KJ
      Molecular biology of the cell 20 328-37 2009

      Show Abstract Full Text Article
      Western BlottingHuman18987342 18987342
      ADAM15 is an adherens junction molecule whose surface expression can be driven by VE-cadherin.
      Ham, Claire, et al.
      Exp. Cell Res., 279: 239-47 (2002) 2002

      Show Abstract
      12243749 12243749

      Data Sheet

      Anti-ADAM 15, C-terminus - Data Sheet