CB1024 Anti-ACTN4 Mouse mAb (4D10)

CB1024
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityHostAntibody Type
      H, M, R M Monoclonal Antibody

      Pricing & Availability

      Catalog Number AvailabilityPackaging Qty/Pack Price Quantity
      CB1024-100UG
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      Discontinued
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          100 μg
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          Description
          OverviewRecognizes the ~90-100 kDa ACTN4 protein in PC-12, HeLa, Raw 264.7, MCF-7, and NIH3T3 cells and human testes tissue.
          Catalogue NumberCB1024
          Brand Family Calbiochem®
          SynonymsAnti-Actinin α4
          Application Data
          Detection of rat ACTN4 by immunoblotting. Sample: Whole cell lysate from PC-12 cells. Primary antibody: Anti-ACTN4 Mouse mAb (4D10) (Cat. No. CB1024) (1-5 µg/ml). Detection: chemiluminescence.

          Detection of Human ACTN4 by immunoblotting. Sample: Whole cell lysate from HeLa NE cells. Primary antibody: Anti-ACTN4 Mouse mAb (4D10) (Cat. No. CB1024) (1-5 µg/ml). Detection: chemiluminescence.

          Detection of mouse ACTN4 by immunoblotting. Sample: Whole cell lysate from Raw 264.7 cells. Primary antibody: Anti-ACTN4 Mouse mAb (4D10) (Cat. No. CB1024) (1-5 µg/ml). Detection: chemiluminescence.

          Detection of Human ACTN4 by immunoblotting. Sample: Whole cell lysate from MCF-7 cells. Priamry antibody: Anti-ACTN4 Mouse mAb (AD10) (Cat. No. CB1024) (1-5 µg/ml). Detection: chemiluminescence.

          Detection of human ACTN4 by staining paraffin sections. Sample: Formalin-fixed, paraffin-embedded human testes tissue. Primary antibody: Anti-ACTN4 Mouse mAb (4D10) (Cat. No. CB1024) (3 µg/ml). Detection: colorimetric (DAB).

          Detection of human ACTN4 by immunocytochemistry. Sample: Paraformaldahyde treated HeLa cells fixed in paraformaldehyde. Primary antibody: Anti-ACTN4 Mouse mAb (4D10) (Cat. No. CB1024) (20 µg/ml). Detection: fluorescence).
          References
          ReferencesYamada, S., et al. 2010. Int. J. Oral Maxillofac. Surg. 39, 61.
          Product Information
          FormLiquid
          FormulationIn PBS, pH 7.2.
          Negative control293T cells
          Positive controlPC-12 cells, HeLa cells, Raw 264.7 cells, MCF-7 cells, NIH3T3 cells, Human testes tissue
          PreservativeNone
          Applications
          Key Applications Paraffin Sections
          Enzyme-Linked Immunosorbent Assay
          Immunoblotting (Western Blotting)
          Immunocytochemistry
          Application NotesELISA (see coments)
          Immunoblotting (1-5 µg/ml)
          Immunocytochemistry (20 µg/ml)
          Paraffin Sections (3 µg/ml)
          Application CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
          Biological Information
          ImmunogenA recombinant polypeptide corresponding to amino acids 592-702 of human ACTN4, espressed as a GST fusion protein
          Clone4D10
          HostMouse
          IsotypeIgG2a
          Species Reactivity
          • Human
          • Mouse
          • Rat
          Antibody TypeMonoclonal Antibody
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Blue Ice Only
          Toxicity Regulatory Review
          Storage -20°C
          Avoid freeze/thaw Avoid freeze/thaw
          Do not freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          Anti-ACTN4 Mouse mAb (4D10) SDS

          Title

          Safety Data Sheet (SDS) 

          Anti-ACTN4 Mouse mAb (4D10) Certificates of Analysis

          TitleLot Number
          CB1024

          References

          Reference overview
          Yamada, S., et al. 2010. Int. J. Oral Maxillofac. Surg. 39, 61.
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision29-October-2010 RFH
          SynonymsAnti-Actinin α4
          ApplicationELISA (see coments)
          Immunoblotting (1-5 µg/ml)
          Immunocytochemistry (20 µg/ml)
          Paraffin Sections (3 µg/ml)
          Application Data
          Detection of rat ACTN4 by immunoblotting. Sample: Whole cell lysate from PC-12 cells. Primary antibody: Anti-ACTN4 Mouse mAb (4D10) (Cat. No. CB1024) (1-5 µg/ml). Detection: chemiluminescence.

          Detection of Human ACTN4 by immunoblotting. Sample: Whole cell lysate from HeLa NE cells. Primary antibody: Anti-ACTN4 Mouse mAb (4D10) (Cat. No. CB1024) (1-5 µg/ml). Detection: chemiluminescence.

          Detection of mouse ACTN4 by immunoblotting. Sample: Whole cell lysate from Raw 264.7 cells. Primary antibody: Anti-ACTN4 Mouse mAb (4D10) (Cat. No. CB1024) (1-5 µg/ml). Detection: chemiluminescence.

          Detection of Human ACTN4 by immunoblotting. Sample: Whole cell lysate from MCF-7 cells. Priamry antibody: Anti-ACTN4 Mouse mAb (AD10) (Cat. No. CB1024) (1-5 µg/ml). Detection: chemiluminescence.

          Detection of human ACTN4 by staining paraffin sections. Sample: Formalin-fixed, paraffin-embedded human testes tissue. Primary antibody: Anti-ACTN4 Mouse mAb (4D10) (Cat. No. CB1024) (3 µg/ml). Detection: colorimetric (DAB).

          Detection of human ACTN4 by immunocytochemistry. Sample: Paraformaldahyde treated HeLa cells fixed in paraformaldehyde. Primary antibody: Anti-ACTN4 Mouse mAb (4D10) (Cat. No. CB1024) (20 µg/ml). Detection: fluorescence).
          DescriptionPurified mouse monoclonal antibody. Recognizes the ~90-100 kDa ACTN4 protein.
          BackgroundAlpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In non-muscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments.
          HostMouse
          ImmunogenA recombinant polypeptide corresponding to amino acids 592-702 of human ACTN4, espressed as a GST fusion protein
          Clone4D10
          IsotypeIgG2a
          Specieshuman, mouse, rat
          Positive controlPC-12 cells, HeLa cells, Raw 264.7 cells, MCF-7 cells, NIH3T3 cells, Human testes tissue
          Negative control293T cells
          FormLiquid
          FormulationIn PBS, pH 7.2.
          PreservativeNone
          CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
          Storage -20°C
          Avoid freeze/thaw
          Do Not Freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
          Toxicity Regulatory Review
          ReferencesYamada, S., et al. 2010. Int. J. Oral Maxillofac. Surg. 39, 61.