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ABT137 | Anti-ACF7/MACF1a Antibody

100 µg  
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      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H IHC, DB, ICC Rb Affinity Purified Polyclonal Antibody
      Catalogue NumberABT137
      DescriptionAnti-ACF7/MACF1a Antibody
      Alternate Names
      • Microtubule-actin cross-linking factor 1, isoforms 1/2/3/5
      • 620 kDa actin-binding protein
      • ABP620
      • Actin cross-linking family protein 7
      • Macrophin-1
      • Trabeculin-alpha
      Background InformationActin cross-linking family protein 7 (ACF7), also known as Microtubule-actin cross-linking factor 1, isoforms 1/2/3/5 (MACF1a) exists as 5 different isoforms. Isoform 2, also known as MACF1a, serves as an F-actin-binding protein, which may play a role in cross-linking actin to other cytoskeletal proteins. In migrating cells, MACF1a localizes to the plasma membrane and ruffles in a GSK3-, Memo-, and APC-dependent manner. MACF1a localization is required for microtubule capture at the cell cortex downstream of ErbB2 signaling. Upon binding with AXIN1, beta-catenin, GSK3beta, and APC, MACF1a translocates this multi-complex from the cytoplasm to the cell membrane. MACF1a acts as a positive regulator of Wnt-induced TCF/beta-catenin-dependent transcriptional activation. It also plays an important role in wound healing, epidermal cellular migration, CNS development, and the polarization and coordinated movement of hair follicle stem cells.
      Product Information
      FormatAffinity Purified
      • Human melanoma and heart tissue
      PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      ApplicationAnti-ACF7/MACF1a is an antibody targeting the ACF7/MACF1a protein, validated for use in IHC & ICC.
      Key Applications
      • Immunohistochemistry
      • Dot Blot
      • Immunocytochemistry
      Application NotesImmunocytochemistry Analysis: A 1:500 dilution from a representative lot detected ACF7/MACF1a in NIH/3T3 and HeLa cells.
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to human ACF7/MACF1a.
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      Species Reactivity
      • Human
      Species Reactivity NoteDemonstrated to react with Human. Other Homologies: Bovine (75% sequence homology).
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • MACF1
      • ABP620
      • ACF7
      Purification MethodAffinity Purfied
      UniProt Number
      Molecular Weight614 kDa calculated. Five isoforms are known to exist. This antibody should recognize isoform 2 (620 kDa), isoform 3 (614 kDa), and isoform 4 (606 kDa).
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Immunohistochemistry in human melanoma and heart tissue.

      Immunohistochemisty Analysis: A 1:250 dilution from a representative lot detected ACF7/MACF1a in human melanoma and heart tissue.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information