MAB312 Anti-A2B5 Antibody, clone A2B5-105

MAB312
100 µg  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      Ma FC, ICC, IF, IHC M Purified Monoclonal Antibody
      Description
      Catalogue NumberMAB312
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-A2B5 Antibody, clone A2B5-105
      Alternate Names
      • Neuron Cell Surface Antigen
      References
      Product Information
      FormatPurified
      Control
      • Type II astrocytes, human neural progenitors
      PresentationLiquid in 0.02M PB, 0.25M NaCl, pH 7.6 containing 0.1% sodium azide.
      Applications
      ApplicationDetect A2B5 using this Anti-A2B5 Antibody, clone A2B5-105 validated for use in FC, IC, IF, IH.
      Key Applications
      • Flow Cytometry
      • Immunocytochemistry
      • Immunofluorescence
      • Immunohistochemistry
      Application NotesIndirect immunofluorescence (1:100 - 1:500) in the detection of neurons in tissue culture.

      Using double immunofluorescence the GQ ganglioside has a similar distribution on neurons to the D2 protein, to the tetanus toxin receptor and to neurofilaments (Walsh, 1980).

      May also be used in the depletion of neurons for a mixed population or their purification by affinity chromatography.

      May also be useful for detecting the metastatic spread of neuroblastoma cells into bone marrow.

      Flow cytometry: live cells {Maric, D. et al. (2000) Cerebral Cortex 10:729-747}.

      Immunohistochemistry: Frozen, fixed tissues (Levison & McCarthy, 1989)

      Complement-mediated cytotoxicity (Eisenbarth et al., 1979)

      Optimal working dilutions must be determined by end user.
      Biological Information
      ImmunogenEmbryonic chicken retinal cells
      CloneA2B5-105
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityReacts with a surface antigen on neurons in the retina, brain, spinal cord and dorsal root ganglia, and with all tested human neuroblastoma cell lines. Does not seem to bind with any leukemic cell lines or with bone marrow cells from patients with leukemia, nor with cells from normal bone marrow. Cytotoxic to neurons in the presence of guinea-pig complement. Protein A binding is positive. Binds with GQ ganglioside on the plasma membrane of neurons of animals of all species tested to date (Eisenbarth, et al., 1979).
      IsotypeIgM
      Species Reactivity
      • Mammals
      Antibody TypeMonoclonal Antibody
      Gene Symbol
      • A2B5
      Purification MethodAmmonium Sulfate Precipitation
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain for 1 year at 2–8°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-A2B5, clone A2B5-105 - 23793702379370
      Anti-A2B5, clone A2B5-105 - 19836821983682
      Anti-A2B5, clone A2B5-105 - 20347072034707
      Anti-A2B5, clone A2B5-105 - 20909322090932
      Anti-A2B5, clone A2B5-105 - 21989592198959
      Anti-A2B5, clone A2B5-105 - 23243932324393
      Anti-A2B5, clone A2B5-105 - LV1458190LV1458190
      Anti-A2B5, clone A2B5-105 - LV1583049LV1583049
      Anti-A2B5, clone A2B5-105 - LV1740106LV1740106
      Anti-A2B5, clone A2B5-105 - LV1765550LV1765550

      References

      Reference overviewApplicationSpeciesPub Med ID
      A phenotypic culture system for the molecular analysis of CNS myelination in the spinal cord.
      Davis, H; Gonzalez, M; Stancescu, M; Love, R; Hickman, JJ; Lambert, S
      Biomaterials  35  8840-5  2014

      Show Abstract
      25064806 25064806
      Analysis of human embryonic stem cells with regulatable expression of the cell adhesion molecule l1 in regeneration after spinal cord injury.
      Yoo, M; Lee, GA; Park, C; Cohen, RI; Schachner, M
      Journal of neurotrauma  31  553-64  2014

      Show Abstract
      24125017 24125017
      Strategies for repair of white matter: influence of osmolarity and microglia on proliferation and apoptosis of oligodendrocyte precursor cells in different basal culture media.
      Kleinsimlinghaus, K; Marx, R; Serdar, M; Bendix, I; Dietzel, ID
      Frontiers in cellular neuroscience  7  277  2013

      Show Abstract
      24421756 24421756
      hESC-derived Olig2(+) progenitors generate a subtype of astroglia with protective effects against ischaemic brain injury.
      Jiang, Peng, et al.
      Nat Commun, 4: 2196 (2013)  2013

      Show Abstract
      23880652 23880652
      Alternating current electric fields of varying frequencies: effects on proliferation and differentiation of porcine neural progenitor cells.
      Lim, JH; McCullen, SD; Piedrahita, JA; Loboa, EG; Olby, NJ
      Cellular reprogramming  15  405-12  2013

      Show Abstract
      23961767 23961767
      Small Molecule Induction of Human Umbilical Stem Cells into MBP-positive Oligodendrocytes in a Defined Three-Dimensional Environment.
      Davis, H; Guo, X; Lambert, S; Stancescu, M; Hickman, JJ
      ACS chemical neuroscience  3  31-39  2012

      Show Abstract
      22582139 22582139
      Rat Cortical Oligodendrocyte-Embryonic Motoneuron Co-Culture: An In Vitro Axon-Oligodendrocyte Interaction Model.
      Davis, H; Gonzalez, M; Bhargava, N; Stancescu, M; Hickman, JJ; Lambert, S
      Journal of biomaterials and tissue engineering  2  206-214  2012

      Show Abstract
      23493660 23493660
      A transgenic mouse model for uromodulin-associated kidney diseases shows specific tubulo-interstitial damage, urinary concentrating defect and renal failure.
      Bernascone I, Janas S, Ikehata M, Trudu M, Corbelli A, Schaeffer C, Rastaldi MP, Devuyst O, Rampoldi L
      Hum Mol Genet  19  2998-3010. Epub 2010 May 14.  2010

      Show Abstract
      20472742 20472742
      The role of endothelin-1 and its receptors in optic nerve head astrocyte proliferation.
      Jeremy A Murphy,Michele L Archibald,Balwantray C Chauhan
      The British journal of ophthalmology  94  2010

      Show Abstract
      20494907 20494907
      Development of a model of sacrocaudal spinal cord injury in cloned Yucatan minipigs for cellular transplantation research.
      Lim, JH; Piedrahita, JA; Jackson, L; Ghashghaei, T; Olby, NJ
      Cellular reprogramming  12  689-97  2010

      Show Abstract Full Text Article
      21108536 21108536

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies