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AB5830 | Anti-8-Hydroxydeoxyguanosine Antibody

AB5830
200 µL  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H ELISA, IH(P) Gt Serum Polyclonal Antibody
      Description
      Catalogue NumberAB5830
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-8-Hydroxydeoxyguanosine Antibody
      Alternate Names
      • 8-OHdG
      • 8-hydroxy-2'-deoxyguanosine
      Background Information8-Hydroxydeoxyguanosine (8OHdG) is a modified base that occurs in DNA due to attack by hydroxyl radicals that are formed as byproducts and intermediates of aerobic metabolism and during oxidative stress. There is increasing evidence to support the involvement of free radical reactions in the damage of biomolecules that eventually lead to several diseases in humans, such as atherosclerosis, cerebral and heart ischemia-reperfusion injury, cancer, rheumatoid arthritis, inflammation, diabetes, aging, and neurodegenerative conditions, such as Alzheimer's disease.

      8OHdG has become increasing popular as a sensitive, stable and integral marker of oxidative damage in cellular DNA. Biomonitoring in humans has demonstrated that 8OHdG can be excreted in the urine, and that a significant increase is caused by exposure to tobacco smoke and ionizing radiation. Because 8OHdG is so well correlated with oxidative stress and damage to DNA, which leads to degenerative disease states, the development of an antibody that can be used to study DNA damage has numerous applications. In addition to the direct study of DNA damage within cells, this antibody has applications in the development of immunoassays that can monitor 8OHdG excretion in the urine and serve as a biomarker of oxidative stress. Industrial uses may extend to the dietary supplement manufacturers, who could benefit from an immunoassay that could be used to test the effectiveness of antioxidants and other nutraceuticals.

      CHEMICON's new goat antiserum to 8-hydroxyguanosine is now available. This antiserum has been shown by ELISA to be completely cross-reactive with 8-hydroxydeoxyguanosine while not cross-reacting with other naturally occurring nucleotides. This antibody should be a valuable tool for scientists working to understand the role of free radical damage in a number of human disease states.
      References
      Product Information
      FormatSerum
      Control
      • Skin, staining is limited to the nucleus.
      PresentationGoat Serum. Liquid containing 0.01% Thimerosal.
      Applications
      ApplicationThis Anti-8-Hydroxydeoxyguanosine Antibody is validated for use in ELISA, IH(P) for the detection of 8-Hydroxydeoxyguanosine.
      Key Applications
      • ELISA
      • Immunohistochemistry (Paraffin)
      Application NotesImmunohistochemistry: >1:200 on paraffin embedded, formalin fixed human brain. It is suggested that the tissues are pretreated with 10 μg/mL of proteinase K for 40 minutes at 37°C prior to staining.

      Dewaxed sections treated with 3%H2O2 in methanol for 30 minutes and blocking was with 10% NGS in TBS. Sections were treated with proteinase K 10ug/ml in PBS pH 7.4, 40 minutes 37C prior to blocking. Detection is via DAB. Since the antibody is staining a nucleic acid it is important to use enough proteinase to penetrate the histone barrier.
      Optimal working dilutions must be determined by the end user.
      Biological Information
      Immunogen8-Hydroxy-2'-deoxyguanosine and 8-hydroxyguanosine.
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostGoat
      SpecificityReacts with 8-Hydroxydeoxyguanosine (8-OHdG) and 8-Hydroxyguanosine (8-OHG). By a competitive ELISA the antibody was 100% inhibited by 8-hydroxydeoxyguanosine and 8-hydroxyguanosine nucleotides. Partial cross-reactivity was observed with 8-mercaptoguanosine (80%) while 8-bromoguanosine showed some cross-reactivity (5%). 2-Deoxyadenosine, 7-methlyguanosine, guanosine monophosphate and guanosine showed no reactivity.
      Species Reactivity
      • Human
      Antibody TypePolyclonal Antibody
      Purification MethodUnpurified
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
      Packaging Information
      Material Size200 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      GOAT ANTI- 8-HYDROXYDEOXYGUANOSINE (8-OHdG) POLYCLONAL ANTIBODY - 2138549 2138549
      GOAT ANTI- 8-HYDROXYDEOXYGUANOSINE (8-OHdG) POLYCLONAL ANTIBODY - 2387568 2387568
      GOAT ANTI- 8-HYDROXYDEOXYGUANOSINE (8-OHdG) POLYCLONAL ANTIBODY - 2424642 2424642
      GOAT ANTI- 8-HYDROXYDEOXYGUANOSINE (8-OHdG) POLYCLONAL ANTIBODY - 2458969 2458969
      GOAT ANTI- 8-HYDROXYDEOXYGUANOSINE (8-OHdG) -2736651 2736651
      GOAT ANTI- 8-HYDROXYDEOXYGUANOSINE (8-OHdG) POLYCLONAL ANTIBODY - 2000794 2000794
      GOAT ANTI- 8-HYDROXYDEOXYGUANOSINE (8-OHdG) POLYCLONAL ANTIBODY - 2034688 2034688
      GOAT ANTI- 8-HYDROXYDEOXYGUANOSINE (8-OHdG) POLYCLONAL ANTIBODY - 2299538 2299538
      GOAT ANTI- 8-HYDROXYDEOXYGUANOSINE (8-OHdG) POLYCLONAL ANTIBODY -2506317 2506317

      References | 17 Available | See All References

      Reference overviewPub Med ID
      Targeted nanoparticles containing the proresolving peptide Ac2-26 protect against advanced atherosclerosis in hypercholesterolemic mice.
      Fredman, G; Kamaly, N; Spolitu, S; Milton, J; Ghorpade, D; Chiasson, R; Kuriakose, G; Perretti, M; Farokhzad, O; Farokzhad, O; Tabas, I
      Science translational medicine 7 275ra20 2015

      Show Abstract
      25695999 25695999
      Macrophage mitochondrial oxidative stress promotes atherosclerosis and nuclear factor-κB-mediated inflammation in macrophages.
      Wang, Y; Wang, GZ; Rabinovitch, PS; Tabas, I
      Circulation research 114 421-33 2014

      Show Abstract
      24297735 24297735
      Selenium-induced antioxidant protection recruits modulation of thioredoxin reductase during excitotoxic/pro-oxidant events in the rat striatum.
      Perla D Maldonado,Verónica Pérez-De La Cruz,Mónica Torres-Ramos,Carlos Silva-Islas,Ramón Lecona-Vargas,Rafael Lugo-Huitrón,Tonali Blanco-Ayala,Perla Ugalde-Muñiz,Gustavo Ignacio Vázquez-Cervantes,Teresa I Fortoul,Syed F Ali,Abel Santamaría
      Neurochemistry international 61 2012

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      22579569 22579569
      Antioxidant sestrin-2 redistribution to neuronal soma in human immunodeficiency virus-associated neurocognitive disorders.
      Soontornniyomkij, V; Soontornniyomkij, B; Moore, DJ; Gouaux, B; Masliah, E; Tung, S; Vinters, HV; Grant, I; Achim, CL
      Journal of neuroimmune pharmacology : the official journal of the Society on NeuroImmune Pharmacology 7 579-90 2012

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      22450766 22450766
      Increased 5S rRNA Oxidation in Alzheimer's Disease.
      Qunxing Ding,Haiyan Zhu,Bing Zhang,Augusto Soriano,Roxanne Burns,William R Markesbery
      Journal of Alzheimer's disease : JAD 29 2012

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      Molecular insights into reprogramming-initiation events mediated by the OSKM gene regulatory network.
      Mah, N; Wang, Y; Liao, MC; Prigione, A; Jozefczuk, J; Lichtner, B; Wolfrum, K; Haltmeier, M; Flöttmann, M; Schaefer, M; Hahn, A; Mrowka, R; Klipp, E; Andrade-Navarro, MA; Adjaye, J
      PloS one 6 e24351 2011

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      Potassium bromate, a potent DNA oxidizing agent, exacerbates germline repeat expansion in a fragile X premutation mouse model.
      Entezam A, Lokanga AR, Le W, Hoffman G, Usdin K
      Hum Mutat 31 611-6. 2010

      Show Abstract Full Text Article
      20213777 20213777
      Stress-induced senescence exaggerates postinjury neointimal formation in the old vasculature.
      Khan, SJ; Pham, S; Wei, Y; Mateo, D; St-Pierre, M; Fletcher, TM; Vazquez-Padron, RI
      American journal of physiology. Heart and circulatory physiology 298 H66-74 2010

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      19855064 19855064
      Loss of fibulin-5 binding to beta1 integrins inhibits tumor growth by increasing the level of ROS.
      Schluterman, Marie K, et al.
      Dis Model Mech 3 333-42 2010

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      20197418 20197418
      Melatonin attenuates unilateral ureteral obstruction-induced renal injury by reducing oxidative stress, iNOS, MAPK, and NF-kB expression.
      Ozbek E, Ilbey YO, Ozbek M, Simsek A, Cekmen M, Somay A
      J Endourol 23 1165-73. 2009

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      19530942 19530942

      Brochure

      Title
      Pathways and Biomarkers of Oxidative Stress (EMD)

      Technical Info

      Title
      White Paper- Modern Methods in Oxidative Stress Research (EMD)

      Data Sheet

      Title
      GOAT ANTI- 8-HYDROXYDEOXYGUANOSINE (8-OHdG) POLYCLONAL ANTIBODY