MAB3802 Anti-53BP1 Antibody, clone BP13

MAB3802
100 µg  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H IF, WB M Purified Monoclonal Antibody
      Description
      Catalogue NumberMAB3802
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-53BP1 Antibody, clone BP13
      Alternate Names
      • p53 Binding Protein 1
      Background InformationP53-binding protein 1 (53BP1) plays a critical role in tumor suppression and is a putative substrate of ATM kinase. Upon DNA damage, it is phosphorylated and relocalizes to the presumptive sites of damage, specifically, double-strand breaks. This also suggests a role in DNA repair, and maintaining genomic stability. It is sequence specific transcription factor that plays a central role in the the response of mammalian cells to genotoxic stress. Also, 53BP1 localizes to kinetochores in mitotic cells, suggesting a potential function in mitotic checkpoint signaling.
      References
      Product Information
      FormatPurified
      PresentationPurified immunoglobulin. Liquid in PBS, containing sodium azide.
      Applications
      ApplicationDetect 53BP1 also known as p53 Binding Protein 1 with Anti-53BP1 Antibody, clone BP13 (Mouse Monoclonal Antibody) validated in IF & WB.
      Key Applications
      • Immunofluorescence
      • Western Blotting
      Application NotesWestern blot. Recognizes a protein of >250 kD. Suggested dilution buffer is TBST. Suggested blocking buffer is 5% non-fat dry milk.

      Immunoprecipitation. Expected size in immunoblot is >250 kD. Suggested lysis buffer is NETN. Suggested capture agent is Protein A + rabbit anti-mouse linker antibody.

      Optimal working dilutions must be determined by the end user.
      Biological Information
      ImmunogenRecombinant human 53BP1 proteins.
      CloneBP13
      HostMouse
      Specificity53BP1
      IsotypeIgG1
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • TP53BP1
      • 53BP1
      • MGC138366
      • p202
      • p53BP1
      • FLJ41424
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: Q12888 # May have a role in checkpoint signaling during mitosis (By similarity). Enhances TP53-mediated transcriptional activation. Binds to sites of DNA damage. Plays a role in the response to DNA damage.
      SIZE: 1972 amino acids; 213574 Da
      SUBUNIT: Interacts with IFI202A (By similarity). Binds to the central domain of TP53/p53. May form homo-oligomers. Interacts with DCLRE1C. Interacts with histone H2AFX and this requires phosphorylation of H2AFX on 'Ser-139'. Interacts with histone H3 that has been methylated on 'Lys-79'. Does not bind unmethylated histone H3.
      SUBCELLULAR LOCATION: Nucleus. Kinetochore. Note=Associated with kinetochores. Both nuclear and cytoplasmic in some cells. Recruited to sites of DNA damage, such as double stand breaks.
      PTM: Asymmetrically dimethylated on Arg residues by PRMT1. Methylation is required for DNA binding. & Phosphorylated in an ATM-dependent manner in response to DNA damage.
      SIMILARITY: SwissProt: Q12888 ## Contains 2 BRCT domains.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain at 2-8°C in undiluted aliquots for up to 12 months from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-53BP1 Antibody, clone BP13 SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-53BP1 Antibody, clone BP13 Certificates of Analysis

      TitleLot Number
      MOUSE ANTI-HUMAN 53BP1 MONOCLONAL ANTIBODY - 23911232391123
      MOUSE ANTI-HUMAN 53BP1 - 25124852512485
      MOUSE ANTI-HUMAN 53BP1 - 31737903173790
      MOUSE ANTI-HUMAN 53BP1 - 32139233213923
      MOUSE ANTI-HUMAN 53BP1 - 32560183256018
      MOUSE ANTI-HUMAN 53BP1 -25522852552285
      MOUSE ANTI-HUMAN 53BP1 -26104762610476
      MOUSE ANTI-HUMAN 53BP1 -26435752643575
      MOUSE ANTI-HUMAN 53BP1 -26839182683918
      MOUSE ANTI-HUMAN 53BP1 -27102582710258

      References

      Reference overviewPub Med ID
      TOPBP1 recruits TOP2A to ultra-fine anaphase bridges to aid in their resolution.
      Broderick, R; Nieminuszczy, J; Blackford, AN; Winczura, A; Niedzwiedz, W
      Nature communications  6  6572  2015

      Show Abstract
      25762097 25762097
      BRCA1 pathway function in basal-like breast cancer cells.
      Hill, SJ; Clark, AP; Silver, DP; Livingston, DM
      Molecular and cellular biology  34  3828-42  2014

      Show Abstract
      25092866 25092866
      FANC pathway promotes UV-induced stalled replication forks recovery by acting both upstream and downstream Polη and Rev1.
      Renaud, E; Rosselli, F
      PloS one  8  e53693  2013

      Show Abstract
      23365640 23365640
      Residual dNA and chromosomal damage in ex vivo irradiated blood lymphocytes correlated with late normal tissue response to breast radiotherapy.
      Chua ML, Somaiah N, A\'hern R, Davies S, Gothard L, Yarnold J, Rothkamm K
      Radiotherapy and oncology : journal of the European Society for Therapeutic Radiology and Oncology  99  362-6. Epub 2011 Jun 23.  2011

      21704405 21704405
      Tumor suppressor p53 binding protein 1 (53BP1) is involved in DNA damage-signaling pathways.
      Rappold, I, et al.
      J. Cell Biol., 153: 613-20 (2001)  2001

      Show Abstract
      11331310 11331310

      Data Sheet

      Title
      Anti-53BP1, clone BP13 - Data Sheet

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies