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AB1864 | Anti-4-1BB Antibody

100 µL  
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      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H FC, WB Rb Serum Polyclonal Antibody
      Catalogue NumberAB1864
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-4-1BB Antibody
      Alternate Names
      • CD137
      Product Information
      PresentationLiquid rabbit serum diluted in PBS, pH 7.4. Contains no preservative.
      ApplicationDetect 4-1BB using this Anti-4-1BB Antibody validated for use in FC & WB.
      Key Applications
      • Flow Cytometry
      • Western Blotting
      Application NotesWestern blotting: 1:2000-1:5000.

      ELISA, direct and indirect: 1:2,000-1:10,000.

      Flow cytometry: 1:1000.

      Optimal working dilutions must be determined by the end user.

      FACS Protocol for AB1864

      1. wash 0.5 -1.0 X 10(6) cells with FACS buffer (see below) 2 times

      2. resuspend cells in 100 μL FACS buffer

      3. add 0.5 - 1.0 μL of undiluted AB1864 and incubate on ice for 1 hour

      for negative control, use pre-immune serum or isotype matched Ig

      4. wash 2 times in FACS buffer

      5. resuspend in 100 μL FACS buffer and add 0.5 μL secondary antibody-FITC conjugate

      6. incubate on ice for 40 min

      7. wash 2 times in FACS buffer and resuspend in 500 μL FACS buffer

      8. FACS analysis

      FACS Buffer

      PBS + 0.2 % BSA + 0.1 % sodium azide

      Filter through 0.45 μm

      Keep at 4°C
      Biological Information
      ImmunogenGST-4-1BB extracellular domain.
      SpecificityHuman 4-1BB a member of the tumor necrosis factor receptor superfamily as a co-stimulatory molecule of T cell activation. By Western blot AB1864 recognizes a 29 kDa protein (Reducing and sdenaturuing conditions). Does not cross-react with mouse 4-1BB.
      Species Reactivity
      • Human
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThe protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor contributes to the clonal expansion, survival, and development of T cells. It can also induce proliferation in peripheral monocytes, enhance T cell apoptosis induced by TCR/CD3 triggered activation, and regulate CD28 co-stimulation to promote Th1 cell responses. The expression of this receptor is induced by lymphocyte activation. TRAF adaptor proteins have been shown to bind to this receptor and transduce the signals leading to activation of NF-kappaB.
      Gene Symbol
      • TNFRSF9
      • CD137
      • 4-1BB
      • OTTHUMP00000044294
      • MGC2172
      • CDw137
      • ILA
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: Q07011 # Receptor for TNFSF14/4-1BBL. Possibly active during T cell activation.
      SIZE: 255 amino acids; 27899 Da
      SUBUNIT: Interacts with TRAF1, TRAF2 and TRAF3. Interacts with LRR-repeat protein 1/LRR-1.
      SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.
      TISSUE SPECIFICITY: Expressed on the surface of activated T-cells.
      SIMILARITY: SwissProt: Q07011 ## Contains 4 TNFR-Cys repeats.
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain at -20°C in undiluted aliquots for up to 6 months from date of receipt. Avoid repeated freeze/thaw cycles.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information



      Reference overviewPub Med ID
      Expression of costimulatory molecules (4-1BBL and Fas) and major histocompatibility class I chain-related A (MICA) in aortic tissue with Takayasu's arteritis.
      Seko, Y; Sugishita, K; Sato, O; Takagi, A; Tada, Y; Matsuo, H; Yagita, H; Okumura, K; Nagai, R
      Journal of vascular research 41 84-90 2004

      Show Abstract
      14752253 14752253
      Heightened intrathecal release of soluble CD137 in patients with multiple sclerosis.
      M K Sharief
      European journal of neurology : the official journal of the European Federation of Neurological Societies 9 49-54 2002

      Show Abstract
      11784376 11784376

      Data Sheet

      RABBIT ANTI-4-1BB (CD137)