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ST1049 | Anti-20S Proteasome α1, 2, 3, 5, 6, & 7-Subunits Mouse mAb (MCP231)

ST1049
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityHostAntibody Type
      H, M, Rb, R, Yeast M Monoclonal Antibody

      Pricing & Availability

      Catalog NumberAvailability Packaging Qty/Pack Price Quantity
      ST1049-100UL
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      Limited AvailabilityLimited Availability
      Stocked 
      Discontinued
      Limited Quantities Available
      Available
        Remaining : Will advise
          Remaining : Will advise
          Will advise
          Contact Customer Service

          Glass bottle 100 ul
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          Description
          OverviewRecognizes α1, 2, 3, 5, 6 and 7 subunits of 20S proteasome in human placental proteasome preparations.
          Catalogue NumberST1049
          Brand Family Calbiochem®
          Application Data
          Detection of human 20S Proteasome α1, 2, 3, 5, 6, 7-subunits by 2D PAGE and immunoblotting. Sample: Whole tissue extract from human placental tissue. Primary antibody: Anti-20S Proteasome α1, 2, 3, 5, 6, & 7-Subunits Mouse mAb (MCP231) (Cat. No. ST1049) (1:1000). Detection: luminography.
          References
          ReferencesKopp, F., et al. 1997. Proc. Natl. Acad. Sci. USA 94, 2939.
          Product Information
          FormLiquid
          FormulationIn PBS.
          Positive controlHuman placental tissue
          Preservative≤0.1% sodium azide
          Applications
          Application ReferencesImmunohistochemistry/Immunofluorescence Kabashi, E., et al. 2004. J. Neurochem. 89, 1325. Frozen Sections Fratta, P., et al. 2005. Am. J. Pathol. 167, 517. Immunocytochemistry/Immunofluorescence Taylor, D.M., et al. 2005. Cell Stress Chaperones 10, 230.
          Key Applications Enzyme-Linked Immunosorbent Assay
          Frozen Sections
          Immunoblotting (Western Blotting)
          Immunocytochemistry
          Immunofluorescence
          Immunohistochemistry
          Not Immunoprecipitation
          Application NotesImmunoblotting (1:1000, chemiluminescence)
          Immunoprecipitation (not recommended)
          ELISA (see comments)
          Immunofluorescence (see application references)
          Immunocytochemistry (see application references)
          Immunohistochemistry (see application references)
          Frozen Sections (see application references)
          Application CommentsImmunoblotting using a human placental proteasome preparation yields two bands at ~29 kDa and ~32 kDa. In ELISA the antibody reacts with the peptide sequence TVWSPQGRLHQVEYAMEA encompassing the prosbox I motif common to α-type subunits. Antibody should be titrated for optimal results in individual systems.
          Biological Information
          Immunogendinitrophenylated proteasomes
          ImmunogenHuman
          Epitopewithin the prosbox I motif common to α subunits (TVWSPQGRLHQVEYAMEA)
          CloneMCP231
          HostMouse
          IsotypeIgG₁
          Species Reactivity
          • Human
          • Mouse
          • Rabbit
          • Rat
          • Yeast
          Antibody TypeMonoclonal Antibody
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Blue Ice Only
          Toxicity Highly Toxic
          Storage -20°C
          Avoid freeze/thaw Avoid freeze/thaw
          Do not freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          SDS

          Title

          Safety Data Sheet (SDS) 

          Certificates of Analysis

          TitleLot Number
          ST1049

          References

          Reference overview
          Kopp, F., et al. 1997. Proc. Natl. Acad. Sci. USA 94, 2939.
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision17-November-2008 JSW
          ApplicationImmunoblotting (1:1000, chemiluminescence)
          Immunoprecipitation (not recommended)
          ELISA (see comments)
          Immunofluorescence (see application references)
          Immunocytochemistry (see application references)
          Immunohistochemistry (see application references)
          Frozen Sections (see application references)
          Application Data
          Detection of human 20S Proteasome α1, 2, 3, 5, 6, 7-subunits by 2D PAGE and immunoblotting. Sample: Whole tissue extract from human placental tissue. Primary antibody: Anti-20S Proteasome α1, 2, 3, 5, 6, & 7-Subunits Mouse mAb (MCP231) (Cat. No. ST1049) (1:1000). Detection: luminography.
          DescriptionPurified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with mouse myleloma cells. Recognizes the ~29-32 kDa α1, 2, 3, 5, 6 and 7 subunits of the 20S proteasome.
          BackgroundProteasomes are large multi-subunit complexes, that are localized in the nucleus and cytosol, and selectively degrade intracellular proteins. They play a major role in the degradation of many proteins involved in cellular processes such as cell cycling, apoptosis, and proliferation. The human proteasome contains two copies of each of seven different α-type and β-type subunits assembled to form a stack of four seven-membered rings. The α-subunits comprise the outer rings while the β-subunits comprise the inner rings. The α-subunits contain information for the binding of regulatory complexes such as PA700 and PA28.
          HostMouse
          Immunogen speciesHuman
          Immunogendinitrophenylated proteasomes
          Epitopewithin the prosbox I motif common to α subunits (TVWSPQGRLHQVEYAMEA)
          CloneMCP231
          IsotypeIgG₁
          Specieshuman, mouse, rabbit, rat, yeast
          Positive controlHuman placental tissue
          FormLiquid
          FormulationIn PBS.
          Preservative≤0.1% sodium azide
          CommentsImmunoblotting using a human placental proteasome preparation yields two bands at ~29 kDa and ~32 kDa. In ELISA the antibody reacts with the peptide sequence TVWSPQGRLHQVEYAMEA encompassing the prosbox I motif common to α-type subunits. Antibody should be titrated for optimal results in individual systems.
          Storage -20°C
          Avoid freeze/thaw
          Do Not Freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
          Toxicity Highly Toxic
          ReferencesKopp, F., et al. 1997. Proc. Natl. Acad. Sci. USA 94, 2939.
          Application referencesImmunohistochemistry/Immunofluorescence Kabashi, E., et al. 2004. J. Neurochem. 89, 1325. Frozen Sections Fratta, P., et al. 2005. Am. J. Pathol. 167, 517. Immunocytochemistry/Immunofluorescence Taylor, D.M., et al. 2005. Cell Stress Chaperones 10, 230.