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04-259 | Anti-19S regulator non-ATPase subunit S5a/Rpn10 Antibody, clone S5a-18

100 µL  
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      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H IP, WB M Purified Monoclonal Antibody
      Catalogue Number04-259
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-19S regulator non-ATPase subunit S5a/Rpn10 Antibody, clone S5a-18
      OverviewRpn10 (S5a) is a non-ATPase subunit of the 19S regulatory complex of the 26s proteasome. Subunit Rpn10 (S5a) of the 26S proteasome was originally identified as a protein capable of binding poly-ubiquitin chains of four or more ubiquitins, in the absence of other 26S proteasome subunits.
      Alternate Names
      • S5a
      Product Information
      Presentation100 µL of purified immunoglobulin in PBS containing 0.01M sodium azide. Dilute to working strength with phosphate buffered saline pH 7.2-7.4.
      ApplicationDetect 19S regulator non-ATPase subunit S5a/Rpn10 using this Anti-19S regulator non-ATPase subunit S5a/Rpn10 Antibody, clone S5a-18 validated for use in IP & WB.
      Key Applications
      • Immunoprecipitation
      • Western Blotting
      Biological Information
      ImmunogenFull length human recombinant Rpn10 protein.
      SpecificityRecognizes 19S regulator non-ATPase subunit S5a/Rpn10.
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThe 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes one of the non-ATPase subunits of the 19S regulator lid. Pseudogenes have been identified on chromosomes 10 and 21.
      Gene Symbol
      • PSMD4
      • pUB-R5
      • OTTHUMP00000059963
      • Rpn10
      • AF-1
      • AF
      • MCB1
      • S5A
      • ASF
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P55036 # Binds and presumably selects ubiquitin-conjugates for destruction. Displays selectivity for longer polyubiquitin chains. Modulates intestinal fluid secretion.
      SIZE: 377 amino acids; 40737 Da
      SUBUNIT: The 26S proteasome is composed of a core protease, known as the 20S proteasome, capped at one or both ends by the 19S regulatory complex (RC). The RC is composed of at least 18 different subunits in two subcomplexes, the base and the lid, which form the portions proximal and distal to the 20S proteolytic core, respectively. Directly interacts with NUB1. Interacts with SQSTM1.
      DOMAIN: SwissProt: P55036 The 2 UIM motifs are involved in the binding to a multi- ubiquitin chain in a cooperative way.
      PTM: Phosphorylated upon DNA damage, probably by ATM or ATR.
      SIMILARITY: Belongs to the proteasome subunit S5A family. & Contains 2 UIM (ubiquitin-interacting motif) repeats. & Contains 1 VWFA domain.
      Molecular Weight41 kDa
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of shipment for up to 12 months.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information




      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-19S regulator non-ATPase subunit S5a/Rpn10, clone S5a-18 - 2042797 2042797


      Reference overviewPub Med ID
      Structural studies of the interaction between ubiquitin family proteins and proteasome subunit S5a
      Walters, Kylie J, et al
      Biochemistry, 41:1767-77 (2002) 2002

      11827521 11827521
      Proteins containing the UBA domain are able to bind to multi-ubiquitin chains
      Wilkinson, C R, et al
      Nat Cell Biol, 3:939-43 (2001) 2001

      11584278 11584278
      Purification of poly-ubiquitinated proteins by S5a-affinity chromatography
      Layfield, R, et al
      Proteomics, 1:773-7 (2001) 2001

      11677784 11677784