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AB9740 | Anti-14-3-3 Antibody, phospho Serine185

100 µL  
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      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      B, H, M, R, Rb, Sh WB Sh Affinity Purified Polyclonal Antibody
      Catalogue NumberAB9740
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-14-3-3 Antibody, phospho Serine185
      Background InformationMammals express seven distinct 14-3-3 isoforms (gamma, epsilon, beta, zeta, sigma, theta, tau) that form multiple homo- and hetero- dimmers, thus rendering 14-3-3 as a key multifunctional regulatory molecule. 14-3-3 isomers have been implicated in several neurological disorders: for example isoforms gamma, epsilon and beta are expressed in the cerebrospinal fluid of Creutzfeldt-Jakob patients, but not patients suffering from other neurological conditions such as Alzheimer's.

      The 14-3-3 phospho Serine185 antibody is one of a panel of antibodies that were generated against 14-3-3 isoforms and have demonstrated their potential for use as an ELISA-based diagnostic tool. These antibodies will allow the research to investigate the role of this protein in key cellular processes and neurological disorders such as Creutzfeldt-Jakob, Alzheimer's and Parkinson's Disease.
      Product Information
      FormatAffinity Purified
      PresentationLiquid in 10 mM Tris-HCl buffer, pH 7.5 with 150 mM NaCl, 100 ug/mL BSA, 50% glycerol and 0.05% sodium azide.
      ApplicationDetect 14-3-3 using this Anti-14-3-3 Antibody, phospho Serine185 validated for use in WB.
      Key Applications
      • Western Blotting
      Application NotesWestern blot: 1:150-1:300 using ECL.

      Optimal working dilutions must be determined by end user.
      Biological Information
      ImmunogenSynthetic phospho peptide.
      Specificity14-3-3 phospho Serine185. By Western blot the antibody recognizes the ~30 kDa protein. Specific for the phosphorylated form of beta and zeta 14-3-3 that are found endogenously phosphorylated only in brain tissue.
      Species Reactivity
      • Bovine
      • Human
      • Mouse
      • Rat
      • Rabbit
      • Sheep
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 100% identical to the mouse ortholog. It interacts with CDC25 phosphatases, RAF1 and IRS1 proteins, suggesting its role in diverse biochemical activities related to signal transduction, such as cell division and regulation of insulin sensitivity. It has also been implicated in the pathogenesis of small cell lung cancer.
      Gene Symbol
      • YWHAE
      • KCIP-1
      • MDS
      • 14-3-3E
      • MDCR
      • FLJ45465
      • Phosphorylation
      Purification MethodImmunoAffinity Purified
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P62258 # Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathway. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner.
      SIZE: 255 amino acids; 29174 Da
      SUBUNIT: Homodimer. Interacts with NDEL1 (By similarity). Interacts with HCV core protein.
      SUBCELLULAR LOCATION: Cytoplasm (By similarity). Melanosome. Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
      SIMILARITY: SwissProt: P62258 ## Belongs to the 14-3-3 family.
      Molecular Weight30 kDa
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain at -20°C in undiluted aliquots for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information




      Safety Data Sheet (SDS) 


      Reference overviewPub Med ID
      14-3-3 alpha and delta are the phosphorylated forms of raf-activating 14-3-3 beta and zeta. In vivo stoichiometric phosphorylation in brain at a Ser-Pro-Glu-Lys MOTIF
      Aitken, A, et al
      J Biol Chem, 270:5706-9 (1995) 1995

      7890696 7890696

      Data Sheet

      Anti-14-3-3 phospho Serine185 - Data Sheet

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      Life Science Research > Antibodies and Assays > Primary Antibodies