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AB9736 | Anti-14-3-3 η Antibody

100 µL  
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      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      B, Ch, H, M, R, Rb, Sh WB, IHC Rb Serum Polyclonal Antibody
      Catalogue NumberAB9736
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-14-3-3 η Antibody
      Background InformationMammals express seven distinct 14-3-3 isoforms (gamma, epsilon, beta, zeta, sigma, eta, tau) that form multiple homo- and hetero- dimmers, thus rendering 14-3-3 as a key multifunctional regulatory molecule. 14-3-3 isomers have been implicated in several neurological disorders: for example isoforms gamma, epsilon and beta are expressed in the cerebrospinal fluid of Creutzfeldt-Jakob patients, but not patients suffering from other neurological conditions such as Alzheimer's.

      The 14-3-3 eta antibody is one of a panel of antibodies that were generated against 14-3-3 isoforms and have demonstrated their potential for use as an ELISA-based diagnostic tool. These antibodies will allow the research to investigate the role of this protein in key cellular processes and neurological disorders such as Creutzfeldt-Jakob, Alzheimer's and Parkinson's Disease.
      Product Information
      ApplicationAnti-14-3-3 η Antibody detects level of 14-3-3 η & has been published & validated for use in WB, IH.
      Key Applications
      • Western Blotting
      • Immunohistochemistry
      Application NotesWestern blot: 1:3,000 using ECL.
      Immunohistochemistry: 1:1,600-1:3,200 on mouse brain tissue which was treated by hydrated autoclaving at 121°C for 15 minutes prior to immunolabeling. Endogenous peroxidase was blocked with hydrogen peroxide in methanol and non-specific labeling with normal goat serum at 1:20 for 5 minutes.

      Optimal working dilutions must be determined by end user.
      Biological Information
      ImmunogenSynthetic peptide from the N-terminal area of sheep 14-3-3 eta.
      Specificity14-3-3 eta. By Western blot the antibody recognizes the ~30 kDa protein.
      Species Reactivity
      • Bovine
      • Chicken
      • Human
      • Mouse
      • Rat
      • Rabbit
      • Sheep
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 99% identical to the mouse, rat and bovine orthologs. This gene contains a 7 bp repeat sequence in its 5' UTR, and changes in the number of this repeat has been associated with early-onset schizophrenia.
      Gene Symbol
      • YWHAH
      • YWHA1
      Purification MethodSerum
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: Q04917 # Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathway. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner.
      SIZE: 246 amino acids; 28219 Da
      SUBUNIT: Homodimer (By similarity). Interacts with many nuclear hormone receptors and cofactors including AR, ESR1, ESR2, MC2R, NR3C1, NRIP1, PPARBP and THRA.
      TISSUE SPECIFICITY: Expressed mainly in the brain and present in other tissues albeit at lower levels.
      SIMILARITY: SwissProt: Q04917 ## Belongs to the 14-3-3 family.
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain at -20°C in undiluted aliquots for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles. During shipment, small volumes of product will occasionally become entrapped in the seal of the product vial. For products with volumes of 200 µL or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container's cap.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information




      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      RABBIT ANTI-14-3-3 eta -2796835 2796835
      RABBIT ANTI-14-3-3 eta POLYCLONAL ANTIBODY - 2280805 2280805


      Reference overviewPub Med ID
      The synaptic proteome during development and plasticity of the mouse visual cortex.
      Dahlhaus, M; Li, KW; van der Schors, RC; Saiepour, MH; van Nierop, P; Heimel, JA; Hermans, JM; Loos, M; Smit, AB; Levelt, CN
      Molecular & cellular proteomics : MCP 10 M110.005413 2011

      Show Abstract Full Text Article
      21398567 21398567
      Unchanged survival rates of 14-3-3gamma knockout mice after inoculation with pathological prion protein.
      Steinacker, Petra, et al.
      Mol. Cell. Biol., 25: 1339-46 (2005) 2005

      Show Abstract
      15684385 15684385
      Mammalian and yeast 14-3-3 isoforms form distinct patterns of dimers in vivo
      Chaudhri, Maliha, et al
      Biochem Biophys Res Commun, 300:679-85 (2003) 2003

      12507503 12507503
      Immunolocalisation of 14-3-3 isoforms in normal and scrapie-infected murine brain
      Baxter, H C, et al
      Neuroscience, 109:5-14 (2002) 2002

      11784696 11784696
      Identification of 14-3-3 proteins in human platelets: effects of synthetic peptides on protein kinase C activation
      Wheeler-Jones, C P, et al
      Biochem J, 315 ( Pt 1):41-7 (1996) 1996

      8670130 8670130
      Antibodies against the major brain isoforms of 14-3-3 protein. An antibody specific for the N-acetylated amino-terminus of a protein
      Martin, H, et al
      FEBS Lett, 331:296-303 (1993) 1993

      8375512 8375512

      Data Sheet

      Anti-14-3-3 eta - Data Sheet