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AB9734 | Anti-14-3-3 ε Antibody, NT

100 µL  
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      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      B, Ch, H, M, R, Rb, Sh WB, IHC Rb Serum Polyclonal Antibody
      Catalogue NumberAB9734
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-14-3-3 ε Antibody, NT
      Background InformationMammals express seven distinct 14-3-3 isoforms (gamma, epsilon, beta, zeta, sigma, theta, tau) that form multiple homo- and hetero- dimmers, thus rendering 14-3-3 as a key multifunctional regulatory molecule. 14-3-3 isomers have been implicated in several neurological disorders: for example isoforms gamma, epsilon and beta are expressed in the cerebrospinal fluid of Creutzfeldt-Jakob patients, but not patients suffering from other neurological conditions such as Alzheimer's.

      The 14-3-3 epsilon, C-terminal antibody is one of a panel of antibodies that were generated against 14-3-3 isoforms and have demonstrated their potential for use as an ELISA-based diagnostic tool. These antibodies will allow the research to investigate the role of this protein in key cellular processes and neurological disorders such as Creutzfeldt-Jakob, Alzheimer's and Parkinson's Disease.
      Product Information
      ApplicationThis Anti-14-3-3 ε Antibody, N-terminal is validated for use in WB, IH for the detection of 14-3-3 ε.
      Key Applications
      • Western Blotting
      • Immunohistochemistry
      Application NotesWestern blot: 1:3,000 using ECL. Immunohistochemistry: 1:200-1:400 on mouse brain tissue which was treated by hydrated autoclaving at 121°C for 15 minutes prior to immunolabeling. Endogenous peroxidase was blocked with hydrogen peroxide in methanol and non-specific labeling with normal goat serum at 1:20 for 5 minutes. ELISA Optimal working dilutions must be determined by end user.
      Biological Information
      ImmunogenSynthetic peptide from the N-terminal area of sheep 14-3-3 epsilon.
      Specificity14-3-3 epsilon, N-terminal. By Western blot the antibody recognizes the ~33 kDa protein.
      Species Reactivity
      • Bovine
      • Chicken
      • Human
      • Mouse
      • Rat
      • Rabbit
      • Sheep
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 100% identical to the mouse ortholog. It interacts with CDC25 phosphatases, RAF1 and IRS1 proteins, suggesting its role in diverse biochemical activities related to signal transduction, such as cell division and regulation of insulin sensitivity. It has also been implicated in the pathogenesis of small cell lung cancer.
      Gene Symbol
      • YWHAE
      • KCIP-1
      • MDS
      • 14-3-3E
      • MDCR
      • FLJ45465
      Purification MethodSerum
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P62258 # Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathway. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner.
      SIZE: 255 amino acids; 29174 Da
      SUBUNIT: Homodimer. Interacts with NDEL1 (By similarity). Interacts with HCV core protein.
      SUBCELLULAR LOCATION: Cytoplasm (By similarity). Melanosome. Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
      SIMILARITY: SwissProt: P62258 ## Belongs to the 14-3-3 family.
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain at -20°C in undiluted aliquots for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information




      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      RABBIT ANTI-14-3-3 epsilon, N-TERMINAL -2570714 2570714


      Reference overviewApplicationPub Med ID
      Differential proteomic analysis of human erythroblasts undergoing apoptosis induced by epo-withdrawal.
      Pellegrin, S; Heesom, KJ; Satchwell, TJ; Hawley, BR; Daniels, G; van den Akker, E; Toye, AM
      PloS one 7 e38356 2012

      Show Abstract
      Western Blotting22723854 22723854
      Interaction of Akt-phosphorylated ataxin-1 with 14-3-3 mediates neurodegeneration in spinocerebellar ataxia type 1
      Chen, Hung-Kai, et al
      Cell, 113:457-68 (2003) 2003

      12757707 12757707
      Immunolocalisation of 14-3-3 isoforms in normal and scrapie-infected murine brain
      Baxter, H C, et al
      Neuroscience, 109:5-14 (2002) 2002

      11784696 11784696
      Isoform pattern of 14-3-3 proteins in the cerebrospinal fluid of patients with Creutzfeldt-Jakob disease
      Wiltfang, J, et al
      J Neurochem, 73:2485-90 (1999) 1999

      10582609 10582609
      Identification of 14-3-3 proteins in human platelets: effects of synthetic peptides on protein kinase C activation
      Wheeler-Jones, C P, et al
      Biochem J, 315 ( Pt 1):41-7 (1996) 1996

      8670130 8670130

      Data Sheet

      Anti-14-3-3 epsilon, N-terminal - Data Sheet

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      Life Science Research > Antibodies and Assays > Primary Antibodies