A part of MilliporeSigma

ABE1352 | Anti- trimethyl Histone H3 (Lys9) Antibody

50 µg  
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      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H WB, ICC, ChIP, ChIP-seq, ELISA, DB Rb Affinity Purified Polyclonal Antibody
      Catalogue NumberABE1352
      DescriptionAnti- trimethyl Histone H3 (Lys9) Antibody
      Alternate Names
      • Histone H3.1
      • Histone H3/a
      • Histone H3/b
      • Histone H3/c
      • Histone H3/d
      • Histone H3/f
      • Histone H3/h
      • Histone H3/I
      • Histone H3/j
      • Histone H3/k
      • Histone H3/l
      Background InformationHistones are highly conserved proteins that serve as the structural scaffold for the organization of nuclear DNA into chromatin. The four core histones, H2A, H2B, H3, and H4, assemble into an octamer (2 molecules of each). Subsequently, 146 base pairs of DNA are wrapped around the octamer, forming a nucleosome. Histones are modified post-translationally by the actions of enzymes in both the nucleus and cytoplasm. These modifications regulate DNA transcription, repair, recombination, and replication. The most commonly studied modifications are acetylation, phosphorylation, methylation, and ubiquitination. Histone modifications can alter local chromatin architecture, or recruit trans-acting factors that recognize specific histone modifications. These modifications occur predominantly on the N-terminal and C-terminal tails that extend beyond the nucleosome core particle. Histone H3 can be mono-, di-, or trimethylated at Lys27 by the action of histone methyltransferases such as EZH2 or NSD3. Methylation of lysine 27 in histone H3 is commonly associated with heterochromatin. and transcriptional repression.
      Product Information
      FormatAffinity Purified
      PresentationAffinity purified rabbit polyclonal in PBS containing 0.05% azide and 0.05% ProClin 300.
      ApplicationThis Anti- trimethyl Histone H3 (Lys9) Antibody is validated for use in Western Blotting and Immunocytochemistry and Chromatin Immunoprecipitation (ChIP) and ChIP-seq and ELISA and Dot Blot for the detection of trimethyl Histone H3 (Lys9).
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      • Chromatin Immunoprecipitation (ChIP)
      • ChIP-seq
      • ELISA
      • Dot Blot
      Application NotesChromatin Immunoprecipitation (ChIP): A representative lot of this antibody (1μg per reaction) was used for ChIP using chromatin from HeLa and K562 cells.

      ChIP-seq: A representative lot of this antibody (1 μg per reaction) was used to immunoprecipitate K562 chromatin for ChIP-seq analysis

      Dot Blot Specificity Analysis: Unmodified and various modified Histone peptides were probed with a representative lot of Anti-trimethyl-Histone H3 (Lys9) (1:20,000 dilution).

      Immunocytochemistry: A 1:500 dilution from a representative lot detected histone H3 trimethylated at lysine 9 in HeLa cells.

      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to region near N-terminus of Human histone H3 trimethylated at Lys9.
      EpitopeNear N-terminus
      ConcentrationPlease refer to lot specific datasheet.
      SpecificityRecognizes histone H3 trimethylated at lysine 9
      Species Reactivity
      • Human
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • HIST1H3A
      • H3FA
      • HIST1H3B
      • H3FL
      • HIST1H3C
      • H3FC
      • HIST1H3D
      • H3FB
      • HIST1H3E
      • H3FD
      • HIST1H3F
      • H3FI
      • HIST1H3G
      • H3FH
      • HIST1H3H
      • H3FK
      • HIST1H3I
      • H3FF
      • HIST1H3J
      • H3FJ
      • Methylation
      Purification MethodAntigen Affinity Purified
      UniProt Number
      Molecular Weight~17 kDa observed
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in a HeLa acid extract.

      Western Blotting Analysis: A 2.7 µg/mL dilution of this antibody detected trimethyl Histone H3 (Lys9) in a HeLa acid extract.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.
      Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size50 µg
      Transport Information
      Supplemental Information