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SCR590 | Amplifluor® Human/Mouse GAPDH Primer Set (JOE labeled)

100 Reactions/Kit  
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      Replacement Information
      Catalogue NumberSCR590
      Trade Name
      • Amplifluor
      DescriptionAmplifluor® Human/Mouse GAPDH Primer Set (JOE labeled)
      Background InformationThe Amplifluor Human/Mouse GAPDH JOE real time PCR primer set was designed for the quantitative evaluation of GAPDH housekeeping gene expression. It can be used in either single gene detection assay or together with other fluorophore conjugated primer sets in multiplex detection assays. Assay conditions may need to be further optimized for multiplex detection.

      This kit utilizes the Amplifluor system for the detection of RNA expression levels. The Amplifluor RT-PCR reaction is performed using a reverse transcription (RT) primer for 1st strand cDNA synthesis, followed by Polymerase Chain Reaction (PCR) amplification utilizing the forward Amplifluor hairpin primer and reverse primer to produce gene-specific fluorescent amplicons. The Amplifluor hairpin primer is labeled with Donor (fluorophore) and Quencher (DABSYL), which produce the high signal to noise ratio observed in the Amplifluor assay. Upon incorporation into new amplicon, the Amplifluor primer is fluorescent, while the fluorophore signal of the unincorporated Amplifluor primer is quenched. When performed on a thermal cycler that is capable of real-time fluorescence detection, the amplification products of the PCR reaction can be monitored directly in a quantitative fashion, allowing evaluation of template detection in a single, closed-tube system with no associated assay procedure. Where required, template standard curves may be employed to quantitatively determine sample load.
      Product Information
      • 1. Amplifluor Conjugated Human/Mouse GAPDH JOE Forward Primer, 25X (Part number CS204430): One (1) vial containing 100 µL (100 reactions).
      • 2. Human/Mouse GAPDH Reverse Primer, 25X (Part number CS204489): One (1) vial containing 100 µL (100 reactions).
      • 3. Positive Control-HN (Part number CS204492): One (1) vial containing 100 µL (10e9 copies/5 µL).
      • 4. Sterile distilled water (Part number 90442): One (1) vial containing 1.3 mL of nuclease-free PCR-grade water.
      ApplicationThe Amplifluor Human/Mouse GAPDH JOE real time PCR primer set was designed for the quantitative evaluation of GAPDH housekeeping gene expression.
      Key Applications
      • PCR
      Biological Information
      SpecificityHuman/Mouse GAPDH
      Stem Cell Type
      • Induced Pluripotent Stem Cells
      • Human Embryonic Stem Cells
      • Mouse Embryonic Stem Cells
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsAmplifluor primers are light sensitive and must be stored in the dark at -20°C or at -80°C for long term storage. Amplifluor primers may be divided into convenient aliquots and placed in amber or foil-wrapped tubes to minimize light exposure. Reverse primers and positive control should be stored at or below -20°C.
      Packaging Information
      Material Size100 Reactions/Kit
      Transport Information
      Supplemental Information