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SCR593 | Amplifluor® Human/Mouse 18S rRNA Primer Set (FAM labeled)

SCR593
100 Reactions/Kit  
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      Overview

      Replacement Information
      Description
      Catalogue NumberSCR593
      Trade Name
      • Amplifluor
      DescriptionAmplifluor® Human/Mouse 18S rRNA Primer Set (FAM labeled)
      Background InformationThe Amplifluor Human/Mouse 18S rRNA FAM real time PCR primer set was designed for the quantitative evaluation of 18S rRNA housekeeping gene expression. It can be used in either single gene detection assay or together with other fluorophore conjugated primer sets in multiplex detection assays. Assay conditions may need to be further optimized for multiplex detection.

      This kit utilizes the Amplifluor system for the detection of RNA expression levels. The Amplifluor RT-PCR reaction is performed using a reverse transcription (RT) primer for 1st strand cDNA synthesis, followed by Polymerase Chain Reaction (PCR) amplification utilizing the forward Amplifluor hairpin primer and reverse primer to produce gene-specific fluorescent amplicons. The Amplifluor hairpin primer is labeled with Donor (fluorophore) and Quencher (DABSYL), which produce the high signal to noise ratio observed in the Amplifluor assay. Upon incorporation into new amplicon, the Amplifluor primer is fluorescent, while the fluorophore signal of the unincorporated Amplifluor primer is quenched. When performed on a thermal cycler that is capable of real-time fluorescence detection, the amplification products of the PCR reaction can be monitored directly in a quantitative fashion, allowing evaluation of template detection in a single, closed-tube system with no associated assay procedure. Where required, template standard curves may be employed to quantitatively determine sample load.
      References
      Product Information
      Components
      • 1. Amplifluor Conjugated Human/Mouse 18S rRNA FAM Forward Primer, 25X (Part number CS204426): One (1) vial containing 100 µL (100 reactions).
      • 2. Human/Mouse 18S rRNA Reverse Primer, 25X (Part number CS204488): One (1) vial containing 100 µL (100 reactions).
      • 3. Positive Control-HN (Part number CS204492): One (1) vial containing 100 µL (10e9 copies/5 µL).
      • 4. Sterile distilled water (Part number 90442): One (1) vial containing 1.3 mL of nuclease-free PCR-grade water.
      Applications
      ApplicationThe Amplifluor Human/Mouse 18S rRNA FAM real time PCR primer set was designed for the quantitative evaluation of 18S rRNA housekeeping gene expression.
      Key Applications
      • PCR
      Biological Information
      SpecificityHuman/Mouse 18S rRNA
      Stem Cell Type
      • Induced Pluripotent Stem Cells
      • Human Embryonic Stem Cells
      • Mouse Embryonic Stem Cells
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsAmplifluor primers are light sensitive and must be stored in the dark at -20°C or at -80°C for long term storage. Amplifluor primers may be divided into convenient aliquots and placed in amber or foil-wrapped tubes to minimize light exposure. Reverse primers and positive control should be stored at or below -20°C.
      Packaging Information
      Material Size100 Reactions/Kit
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Certificates of Analysis

      TitleLot Number
      Amplifluor#174; Human/Mouse 18S rRNA Primer Set (FAM labeled) - 2060977 2060977
      Amplifluor#174; Human/Mouse 18S rRNA Primer Set (FAM labeled) - NRG1788086 NRG1788086
      Amplifluor® Human/Mouse 18S rRNA Primer Set (FAM labeled) - 2377384 2377384
      Amplifluor® Human/Mouse 18S rRNA Primer Set (FAM labeled) - 2496217 2496217

      Data Sheet

      Title
      Reprogramming Cell Fate and Function Novel Strategies for iPSC Generation, Characterization, and Differentiation