ACS10000 Amnis® NFkB Translocation Kit

ACS10000
50 Tests / Kit  
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      Overview

      Replacement Information
      Description
      Catalogue NumberACS10000
      Trade Name
      • Amnis
      DescriptionAmnis® NFkB Translocation Kit
      OverviewThe Amnis NFkB translocation kit includes the conjugated antibody Anti-Hu NFkB (p50) Alexa Fluor 488, the nuclear stain 7-AAD along with the fixation solution, assay buffer and permeabilization buffer to rapidly detect and quantitate NFkB's translocation from the cytoplasm to the nucleus.
      Alternate Names
      • Amnis Cell Signaling
      Background InformationNuclear Factor kappa B (NFκB) transcription factor plays a central role in regulating many key processes in mammalian cells, including differentiation, cell growth, inflammation, immunity, and stress responses. The p50/p65 heterodimer is considered to be the most abundant form of NFκB. NFκB is held in an inactive state in the cytoplasm by its inhibitor IkB. Phosphorylation of serine residues on the IkB proteins marks them for degradation via ubiquitination, therefore allowing activation of the NFκB complex. Activated NFκB complex translocates into the nucleus. The Anti-Hu NFκB Alexa Fluor® 488 antibody recognizes NFκB p50 which is a component of the NFκB p50/p65 heterodimer.The Amnis NFkB translocation kit includes the conjugated antibody Anti-Hu NFkB (p50) Alexa Fluor® 488, the nuclear stain 7-AAD alonge with the fixation solution, assay buffer and permeabilization buffer. This 2 color imaging flow cytometry kit along with the Amnis imaging cytometry platforms are designed to detect and quantitate NFkB translocation from the cytoplasm to the nucleus.
      References
      Product Information
      Components
      • Anti-Hu NFκB(p50) Alexa Fluor® 488 (Part Number 4700-1674, 50 tests/vial)
      • 7-AAD Reagent (Part Number 4000-0290, 50 tests/vial)
      • 5X Fixation Solution (Part Number 4300-0340, 2-3mL vials)
      • 5X Assay Buffer (Part Number CS202124, 50mL/vial)
      • Permeabilization Buffer (Part Number CS202125, 10mL/vial)
      Applications
      ApplicationThe Amnis NFkB translocation kit includes the conjugated antibody Anti-Hu NFkB (p50) Alexa Fluor 488, the nuclear stain 7-AAD along with the fixation solution, assay buffer and permeabilization buffer to rapidly detect and quantitate NFkB's translocation from the cytoplasm to the nucleus.
      Key Applications
      • Flow Cytometry
      • Imaging Flow Cytometry
      Biological Information
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceLot to lot in-process testing on all kit components
      Usage Statement
      • Alexa Fluor® is a registered trademark of Molecular Probes, Inc.
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsAnti-Hu NFκB Alexa Fluor® 488, 7-AAD Solution, 5X Assay Buffer, and Permeabilization Buffer at 2-8 °C. Store the 5X Fixation Solution at room temperature.
      Packaging Information
      Material Size50 Tests / Kit
      Transport Information
      Supplemental Information
      Specifications

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      Categories

      Life Science Research > Antibodies and Assays > Antibody Kits