164599 | Aminopeptidase N, Rat

164599
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      Overview

      Replacement Information

      Key Specifications Table

      Pricing & Availability

      Catalog NumberAvailability Packaging Qty/Pack Price Quantity
      164599-10U
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          Plastic ampoule 10 u
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          Description
          OverviewNative aminopeptidase N purified from rat renal brush-border membranes and cleaved with papain to produce soluble enzyme. A single-chain, integral membrane protease that, functions with broad specificity to remove N-terminal amino acids from peptides. It has been reported that levels of aminopeptidase N are elevated in cancer subjects and correlate with tumor load, and thus, the enzyme is believed to play a role in angiogenesis.
          Catalogue Number164599
          Brand Family Calbiochem®
          SynonymsAPN/CD13, aminopeptidase N
          References
          ReferencesBhagwat, S.V., et al. 2003. Blood 101, 1818.
          van Hensbergen, Y., et al. 2002. Clin. Cancer Res. 8, 3747.
          Ishii, K., et al. 2001. Biol. Pharm. Bull. 24, 226.
          Product Information
          Unit of DefinitionOne unit is defined as the amount of enzyme that will hydrolyze 1.0 µmol L-Leu-<i>p</i>NA per min at 37°C, pH 7.2
          EC number3.4.11.2
          FormLiquid
          FormulationIn 200 mM NaCl, 50 mM Tris-HCl, pH 8.0.
          Applications
          Biological Information
          Purity≥90% by SDS-PAGE
          Specific Activity≥100 units/mg protein
          Concentration Label Please refer to vial label for lot-specific concentration
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Dry Ice Only
          Toxicity Standard Handling
          Storage ≤ -70°C
          Avoid freeze/thaw Avoid freeze/thaw
          Do not freeze Ok to freeze
          Special InstructionsFollowing initial thaw aliquot and freeze (-70°C).
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          SDS

          Title

          Safety Data Sheet (SDS) 

          Certificates of Analysis

          TitleLot Number
          164599

          References

          Reference overview
          Bhagwat, S.V., et al. 2003. Blood 101, 1818.
          van Hensbergen, Y., et al. 2002. Clin. Cancer Res. 8, 3747.
          Ishii, K., et al. 2001. Biol. Pharm. Bull. 24, 226.
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision10-April-2008 RFH
          SynonymsAPN/CD13, aminopeptidase N
          DescriptionNative aminopeptidase N purified from rat renal brush-border membranes and cleaved with papain to produce soluble enzyme. A single-chain, integral membrane protease that functions to remove N-terminal amino acids from peptides with broad specificity. Aminopeptidase N is believed to play a role in angiogenesis and elevated levels of aminopeptidase N have been reported in patients with various types of cancer and have been correlated with tumor load. Also involved in the final stages of digestion of small peptides in the intestinal brush border.
          FormLiquid
          FormulationIn 200 mM NaCl, 50 mM Tris-HCl, pH 8.0.
          Concentration Label Please refer to vial label for lot-specific concentration
          Recommended reaction conditions200 mM Tris-HCl, pH 7.2, 100 µl substrate (L-leu-pNA; final concentration 1 mM) in 200 mM Tris-HCl, pH 7.2, enzyme sample, final volume 200 µl; incubate at 37°C for 10 min.*; cleavage is monitored at 405 nm. *Note: Incubation time of 10 min. is provided as a guideline. The incubation time depends on the activity of the enzyme and the sensitivity of the instrument and should be determined for individual assays.
          EC number3.4.11.2
          Purity≥90% by SDS-PAGE
          Specific activity≥100 units/mg protein
          Unit definitionOne unit is defined as the amount of enzyme that will hydrolyze 1.0 µmol L-Leu-pNA per min at 37°C, pH 7.2
          Storage ≤ -70°C
          Avoid freeze/thaw
          Do Not Freeze Ok to freeze
          Special InstructionsFollowing initial thaw aliquot and freeze (-70°C).
          Toxicity Standard Handling
          ReferencesBhagwat, S.V., et al. 2003. Blood 101, 1818.
          van Hensbergen, Y., et al. 2002. Clin. Cancer Res. 8, 3747.
          Ishii, K., et al. 2001. Biol. Pharm. Bull. 24, 226.