16-671 AbSurance™ Pro Histone Peptide Microarray

One Array -up to two assays per array  
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      Replacement Information
      Catalogue Number16-671
      Trade Name
      • AbSurance
      DescriptionAbSurance™ Pro Histone Peptide Microarray
      OverviewFeatures and Benefits
      • Rapid, high content interaction and specificity analysis of chromatin readers, writers, erasers, and histone specific antibodies
      • Perform one or two separate experiments per array using provided adhesive gasket
      • Includes over 270 different peptide sequences for comprehensive binding and specificity analysis
      • All peptides spotted 12 times per array to allow generation of statistically relevant data
      • Use single array to analyze combinatorial histone code –includes biologically relevant post-translational histone modifications as unmodified, singly modified , and multiply modified peptide sequences.
      • Each peptide spotted with fluorescent spotting tracer to enable data normalization and elimination of false negative results
      • Guaranteed full length peptides –all peptides purified by HPLC and analyzed by mass spectrometry prior to spotting.
      • Visualize data using fluorescent or chemiluminescent readouts
      • Readily analyze data using freely available analysis tools

      The AbSurance™ Pro Histone Peptide Microarray is ideal for protein interaction analysis assays or histone antibody specificity screening using scanning densitometry or chemiluminescent detection. This expertly designed glass slide array contains peptides corresponding to unmodified and post-translationally modified sequences of all four core histone subunits (H2A, H2B, H3, H4) plus select variants. Designed to mimic post-translational modifications found in naturally occurring histones, multiple ~20mer peptides are spotted as unmodified, singly modified as well as multiply modified sequences with up to six modifications per peptide. Additional peptides representing common protein epitope tags are also printed for use as controls when screening the interactions of epitope tagged proteins.

      AbSurance Pro Histone Peptide Microarray slides are printed as two identical sub-arrays (see figures) with each of the 270 peptides represented six times on each subarray to ensure reliable and quantitative binding data. This sub-array format also enables analysis of up to two binding experiments per glass slide using the provided two chamber adhesive gasket (see figures). This convenient format allows side by side interaction analysis of histone associated proteins (e.g. chromatin readers, writers, and erasers) in the absence or presence of small molecules or other protein complex members (see figures). Alternatively, this array can be used for comprehensive analysis of histone antibody specificity.

      For a macroarray analysis of histone antibody specificity using single modification sites and a simple western blot like detection approach, the PVDF-FL membrane—based AbSurance™ Complete macroarray (cat# 16-668) is recommended.
      Materials Required but Not DeliveredReagents
      • Histone interacting protein (e.g bromodomain, chromodomain, PHD domamin protein)
      • Primary Antibody for target protein
      • Secondary Antibody
      • Common buffers and reagents (phosphate-buffered saline , BSA, etc.)

      Equipment and supplies
      • Microarray scanner capable of minimum 25 µm resolution
      • Microscope slide dish, Coplin jar or glass array tube for washing slide
      • 24x60mm glass coverslip if not using provided hybridization gasket
      • Standard laboratory pipettes and tips
      Product Information
      Detection methodFluorescence Chemiluminescence
      PresentationEach kit includes an AbSurance Pro Histone Peptide Microarray slide, an Adhesive Two-Chamber Gasket, and 20 Adhesive Sealing Tabs.
      ApplicationHistone peptide array containing 270 unique histone peptides corresponding to unmodified and post-translationally modified sequences of all four core histone subunits (H2A, H2B, H3, H4) plus select variants. Study histone readers, writers, erasers and histone antibody specificity screening.
      Biological Information
      • Acetylation
      • Methylation
      • Phosphorylation
      • Ubiquitylation
      • Citrullination
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStore iin dry environment at 4°C and protect from light and dust. Performance is guaranteed for 3 months from date of receipt.
      Packaging Information
      Material SizeOne Array -up to two assays per array
      Transport Information
      Supplemental Information