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AX45010 | AXIS™ Axon Isolation Device, 450 µm

AX45010
2 packs (5 devices/pack)  
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      Overview

      Replacement Information

      Key Specifications Table

      Key Applications
      CULT
      Description
      Catalogue NumberAX45010
      Trade Name
      • AXIS
      DescriptionAXIS™ Axon Isolation Device, 450 µm
      OverviewAXIS™ Axon Isolation Devices are microfluidic tools that enable researchers to easily isolate, observe, and test developing neurites. They are currently being offered in four different formats to provide test flexibility during investigation.

      AX45010 consists of 10 units of AX450, an axon isolation device consisting of four wells, two channels, and a set of microgrooves. Two of the wells are interconnected by a channel on each side of the device. The microgrooves are located in the area between the two channels and their length is 450µm. Each microgroove is approximately 5µm in height by 10µm in width and are designed to allow neurite passage to traverse through while preventing cell bodies from flowing across the device. There are roughly 120 microgrooves in each AX450 device.

      The AXIS Axon Isolation Devices have been shown to effectively isolate cell bodies from differentiating neurites for a variety of cell types (Taylor et al., 2005; Park et al., 2006; Liu et al., 2008; Yang, et al., 2009; Taylor et al., 2009) and will likely work with most neuronal cell type that can be successfully grown in tissue culture. Because the AXIS devices are made from an inert and non-toxic polymer, cultures grown within AXIS devices can be maintained for weeks at a time. (Chun et al., 1992; Shibayama et al., 1991). In addition, because the polymer used to fabricate the axon isolation devices is optically clear, cells cultured within the device can be imaged with high resolution microscopy, including live cell imaging, confocal, and differential interference microscopy (Taylor et al., 2005; Park et al., 2006; Liu et al., 2008; Yang, et al., 2009; Taylor et al., 2009).

      The microfluidic design of the AXIS devices is highly conducive to generation of hydrostatic pressure, which can be selectively used to isolate a solution, as well as chemical targets of interest, on a designated side of the device (Park et al., 2006). In addition, immunocytochemistry (IHC) can also be performed within an AXIS Axon Isolation device and a protocol to do so is provided in the manual.
      Alternate Names
      • AXIS™ Axon Investigation Platform
      Background InformationThe development of the vertebrate nervous system entails the generation and differentiation of neurons, establishment of signaling circuits, and the formation of numerous synapses. In particular, the formation of axons and dendrites during differentiation has been an area of intense interest. Although tremendous progress has been made in this area, neuronal outgrowth studies have been hampered by the absence of a solid, easy to use, neurite culturing platform which physically orients the cells during investigation. Traditional outgrowth experiments, conducted either by culturing neural stem cells, primary neurons, or tissue slices, typically result in random, overlapping neurite extensions. The ability to address questions surrounding the specific roles of axons, dendrites and somas in neuron differentiation and growth requires a faster, more specialized method that allows scientists to spatially isolate and study individual neurites and their function. To this end, Millipore now offers its most advanced tool for the isolation, visualization, and characterization of neurite outgrowth, the AXon Investigation System (AXIS™). AXIS™ is a unique, easy to use, slide-mounted microfluidic platform that allows culture, regulation, and directional differentiation of neuronal cells via a system of growth chambers and interconnected channels. AXIS devices physically isolate developing neurites from each other and their respective neural cell bodies. In addition, the use of hydrostatic pressure during analysis provides researchers with an opportunity for targeted exposure of neuronal parts to growth factors, drug compounds, or other reagents of biological interest. This ability to selectively control exposure of neuronal outgrowth makes AXIS a powerful platform for any researcher interested in understanding axons, dendrites, somas, and their roles in synaptic formation, neural cell development, differentiation, regeneration, degeneration, and trauma.
      Materials Required but Not Delivered1. Tissue culture instruments/supplies (37ºC incubator with CO2, laminar flow/tissue culture hood, growth media, flasks, etc.)
      2. Sterile 1X PBS (Millipore Cat No BSS-1005-B)
      3. Sterile Milli-Q™ water (Millipore Cat No TMS-006-B)
      4. Glass slides (25 X 75 mm) and coverslips (24 X 60 mm)
      5. Human Collagen Type II (Millipore Cat No CC052), Laminin (Millipore Cat No CC095), Poly-D-Lysine(Millipore Cat No A-003-E), or some other cell attachment solution
      6. 70% ethanol
      7. 4% paraformaldehyde
      8. Sterile plastic petri dishes (100 X 15 mm or larger)
      9. Light Diagnostic Mounting Media (Millipore Cat No 5013)
      10. DAPI (Millipore Cat No S7113)
      11. Flat Jaw Forceps (like those used for holding western blot membranes)
      12. Sonicator
      References
      Product Information
      Components
      • 10 devices of AX450
      Applications
      ApplicationThe AXIS Axon Isolation Devices have been shown to effectively isolate cell bodies from differentiating neurites for a variety of cell types.
      Key Applications
      • Cell Culture
      Biological Information
      Cell Line Type
      • Neural Lineage Cells
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEach unit is visually inspected to conform with internal specifications for channel width, length, and height.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsThe AXIS devices should be stored at room temperature in a clean dry area away from any chemicals or direct light. When stored properly the devices are stable for 6 months from date of receipt. Discard any remaining reagents after the expiration date.
      Packaging Information
      Material Size2 packs (5 devices/pack)
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      References

      Reference overviewPub Med ID
      Stable kinesin and dynein assemblies drive the axonal transport of mammalian prion protein vesicles.
      Encalada, Sandra E, et al.
      Cell, 144: 551-65 (2011) 2011

      Show Abstract
      21335237 21335237
      Presynaptic regulation of astroglial excitatory neurotransmitter transporter GLT1.
      Yang, Yongjie, et al.
      Neuron, 61: 880-94 (2009) 2009

      Show Abstract
      19323997 19323997
      Axonal mRNA in uninjured and regenerating cortical mammalian axons.
      Taylor, Anne M, et al.
      J. Neurosci., 29: 4697-707 (2009) 2009

      Show Abstract
      19369540 19369540
      A microfluidic chamber for analysis of neuron-to-cell spread and axonal transport of an alpha-herpesvirus.
      Liu, Wendy W, et al.
      PLoS ONE, 3: e2382 (2008) 2008

      Show Abstract
      18560518 18560518
      Microfluidic culture platform for neuroscience research.
      Park, Jeong Won, et al.
      Nat Protoc, 1: 2128-36 (2006) 2006

      Show Abstract
      17487204 17487204
      A microfluidic culture platform for CNS axonal injury, regeneration and transport.
      Taylor, Anne M, et al.
      Nat. Methods, 2: 599-605 (2005) 2005

      Show Abstract
      16094385 16094385

      Brochure

      Title
      AXIS: Axon Investigation System

      Posters

      Title
      Investigation of Axon Growth and Development Using Two Novel Assay Formats

      FAQ

      QuestionAnswer
      The Axonal Isolation System - Frequently Asked Questions Visit our Axonal Isolation System FAQs.

      User Guides

      Title
      AXIS™: AXon Investigation System