A part of MilliporeSigma

12-305 | 3T3/A31 Cell Lysate

100 µL  
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      Replacement Information

      Key Specifications Table

      Key Applications
      Catalogue Number12-305
      Brand Family Upstate
      Trade Name
      • Upstate
      Description3T3/A31 Cell Lysate
      Product Information
      PresentationModified RIPA buffer (50mM Tris-HCl, pH 7.4, 1% NP40, 0.25% sodium deoxycholate, 150mM NaCl, 1mM EDTA, 1mM PMSF, 1mg/ml aprotinin, 1mg/ml leupeptin, 1mg/ml pepstatin, 1mM Na3VO4, 1mM NaF) and diluted with non-reducing sample buffer (31mM Tris-HCl, pH 6.8, 5% glycerol, 1% SDS, 0.002% bromphenol blue).
      Key Applications
      • Western Blotting
      Biological Information
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated as a positive control for some of Upstates antibodies.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions6 months at -20°C
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information


      Certificates of Analysis

      TitleLot Number
      3T3/A31 Cell Lysate 2465218
      3T3/A31 Cell Lysate (murine fibroblast cell line - 2136487 2136487
      3T3/A31 Cell Lysate (murine fibroblast cell line) - 12-305 12-305
      3T3/A31 Cell Lysate (murine fibroblast cell line) - 2136488 2136488
      3T3/A31 Cell Lysate (murine fibroblast cell line) - 2395730 2395730
      3T3/A31 Cell Lysate (murine fibroblast cell line) - 1992471 1992471
      3T3/A31 Cell Lysate (murine fibroblast cell line) - 2023288 2023288
      3T3/A31 Cell Lysate (murine fibroblast cell line) - 2064524 2064524
      3T3/A31 Cell Lysate (murine fibroblast cell line) - 2326370 2326370
      3T3/A31 Cell Lysate - 0608038487 0608038487
      3T3/A31 Cell Lysate - 0703054084 0703054084
      3T3/A31 Cell Lysate - 27837 27837
      3T3/A31 Cell Lysate - 29934 29934
      3T3/A31 Cell Lysate - 31715 31715
      3T3/A31 Cell Lysate - 32680 32680
      3T3/A31 Cell Lysate - Any-lot Any-lot
      3T3/A31 Cell Lysate - DAM1493358 DAM1493358
      3T3/A31 Cell Lysate - DAM1564473 DAM1564473
      3T3/A31 Cell Lysate - DAM1638623 DAM1638623
      3T3/A31 Cell Lysate - DAM1683506 DAM1683506
      3T3/A31 Cell Lysate - DAM1713308 DAM1713308
      3T3/A31 Cell Lysate - DAM1808144 DAM1808144
      3T3/A31 Cell Lysate - NG1951655 NG1951655
      3T3/A31 Cell Lysate -2510303 2510303
      3T3/A31 Cell Lysate -2605384 2605384
      3T3/A31 Cell Lysate -2664288 2664288
      3T3/A31 Cell Lysate -2700850 2700850
      3T3/A31 Cell Lysate -2723765 2723765
      3T3/A31 Cell Lysate -2768455 2768455


      Reference overviewApplicationPub Med ID
      Mechanism of ribosomal p70S6 kinase activation by granulocyte macrophage colony-stimulating factor in neutrophils: cooperation of a MEK-related, THR421/SER424 kinase and a rapamycin-sensitive, m-TOR-related THR389 kinase.
      Lehman, Jason A, et al.
      J. Biol. Chem., 278: 28130-8 (2003) 2003

      Show Abstract
      Kinase Assay12740386 12740386