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ECM675 | 3D Collagen Culture Kit

ECM675
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      Overview

      Replacement Information

      Key Specifications Table

      Key Applications
      EA
      Description
      Catalogue NumberECM675
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      Description3D Collagen Culture Kit
      OverviewCell migration is a fundamental function of normal cellular processes, including cell proliferation, and migration. Cell behavior in vitro is typically examined in a 2D environment; however, in the body, cells exist in a three-dimensional extracellular matrix environment rich in type I collagen. In fact, cells cultured in 3D matrices better reflect in vivo cell physiology when compared to traditional 2D systems.

      CHEMICON (EMD Millipore) has developed a three-dimensional cell culture system that closely simulates the collagen-rich extracellular matrix of normal tissue. This 3D system provides a simple and rapid method to analyze cell angiogenesis, migration, apoptosis, proliferation and tissue formation in a 3D-collagen matrix. Cells suspended in this 3D system are easily visualized by phase contrast or fluorescence light microscopy. Cells can be directly fixed and stained within the matrix and treated with antibodies for visualization of specific intra- and extracellular proteins. Importantly, cells suspended in our 3D system can also be treated with various reagents allowing for extensive screening of biological responses to growth factors and chemical agents.

      As an additional benefit to investigators, sterile and viable cells may be removed from the 3D Cell Culture System for further experimentation, including FACS or biochemical analysis.

      For Research Use Only; Not for use in diagnostic procedures
      Materials Required but Not Delivered· Tissue Culture Dishes

      · Pipettes and Sterile Tips

      · Microcentrifuge Tubes, Sterile

      · 37oC Incubator

      · Inverted Light Microscope

      · Bacterial Collagenase
      References
      Product Information
      Components
      • Collagen Solution - (Part No. 90135) One bottle - 10 mL
      • 5X RPMI Medium- (Part No. 90134) One bottle - 2.5 mL
      • 5X M199 Medium- (Part No. 90133) One bottle - 2.5 mL
      • 5X DMEM Medium- (Part No. 90137) One bottle - 2.5 mL
      • 5X PBS with Phenol Red- (Part No. 90136) One bottle - 2.5 mL
      • Neutralization Solution- (Part No. 90138) One vial - 0.5 mL
      PresentationCollagen solution is supplied in 6mM acetate buffer at ~ pH7.0.
      Applications
      Key Applications
      • Enzyme Assay
      Application NotesThis 3D system provides a simple and rapid method to analyze cell angiogenesis, migration, apoptosis, proliferation and tissue formation in a 3D-collagen matrix. Cells suspended in this 3D system are easily visualized by phase contrast or fluorescence light microscopy. Cells can be directly fixed and stained within the matrix and treated with antibodies for visualization of specific intra- and extracellular proteins. Importantly, cells suspended in our 3D system can also be treated with various reagents allowing for extensive screening of biological responses to growth factors and chemical agents.

      As an additional benefit to investigators, sterile and viable cells may be removed from the 3D Cell Culture System for further experimentation, including FACS or biochemical analysis.
      Biological Information
      Species Reactivity
      • Human
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStore kit materials at 4°C up to their expiration date.
      Packaging Information
      Material Size1 kit
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Protocols

      Title
      3D Collagen Cell Culture System Visual Guide

      References

      Reference overviewPub Med ID
      Synergism of Human Amnion-derived Multipotent Progenitor (AMP) Cells and a Collagen Scaffold in Promoting Brain Wound Recovery: Pre-clinical Studies in an Experimental Model of Penetrating Ballistic-like Brain Injury.
      Chen Z, Lu XC, Shear DA, Dave JR, Davis AR, Evangelista CA, Duffy D, Tortella FC
      Brain Res 2010

      Show Abstract
      20951684 20951684
      Inhibition of neurotrophin receptor p75 intramembran proteolysis by gamma-secretase inhibitor reduces medulloblastoma spinal metastasis.
      Xiaoji Wang,Min Cui,Lei Wang,XueHong Chen,Pan Xin
      Biochemical and biophysical research communications 403 2010

      Show Abstract
      21073863 21073863
      Gamma-secretase represents a therapeutic target for the treatment of invasive glioma mediated by the p75 neurotrophin receptor.
      LiMei Wang, Jennifer J Rahn, XueQing Lun, Beichen Sun, John J P Kelly, Samuel Weiss, Stephen M Robbins, Peter A Forsyth, Donna L Senger
      PLoS biology 6 e289 2008

      Show Abstract Full Text Article
      19067488 19067488

      Data Sheet

      Title
      3D Collagen Culture Kit - Data Sheet

      User Guides

      Title
      3D Collagen Culture Kit

      Related Products & Applications

      Product Families

      Categories

      Life Science Research > Cell Analysis > Cell-based Assays > Cell Migration Assays