480716 | α2-3,6,8,9-Neuraminidase, Arthrobacter ureafaciens, Recombinant, E. coli

480716
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      Overview

      Replacement Information

      Key Specifications Table

      Pricing & Availability

      Catalog NumberAvailability Packaging Qty/Pack Price Quantity
      480716-250MIU
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          Plastic ampoule 250 miu
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          Description
          OverviewRecombinant, Arthrobacter ureafaciens α2-3,6,8,9-Neuraminidase expressed in E. coli. Catalyzes the hydrolysis of all non-reducing terminal sialic acid residues from complex carbohydrates and glycoproteins. High concentrations of this sialidase and prolonged periods of incubation result in the additional cleavage of branched sialic acids. This enzyme is found as two active species of MW 60000 and 69000.
          Note: 1 mU = 1 milliunit.
          Catalogue Number480716
          Brand Family Calbiochem®
          SynonymsN-Acetylneuraminyl Hydrolase, Sialidase
          References
          ReferencesMeans, R.E. and Desrosiers, R.C. 2000. J. Virol. 74, 11181.
          Prime, S., et al. 1996. J. Chromatogr. A. 720, 263.
          Roggentin, P., et al. 1995. Biol. Chem. Hoppe Seyler 376, 569
          Dwek, R.A., et al. 1993. Annu. Rev. Biochem. 62, 65.
          Product Information
          CAS number9001-67-6
          Activity≥5 units/ml
          Unit of DefinitionOne unit is defined as the amount of enzyme that will catalyze the release of 1 µmol of methylumbelliferone from 2ʹ-(4-methylumbelliferyl)-α-D-N-acetylneuraminic acid per min at 37°C, pH 5.0. (Note: 1 mU = 1 milliunit).
          EC number3.2.1.18
          FormLiquid
          FormulationIn 20 mM Tris-HCl, 20 mM NaCl, pH 7.5.
          Applications
          Biological Information
          Specific Activity≥40 units/mg protein
          Physicochemical Information
          ContaminantsN-acetylglucosaminidase, α-fucosidase, α- and β-galactosidase, α- and β-mannosidase, proteases: none detected.
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          RTECSQQ3450000
          Safety Information
          R PhraseR: 20-36/37/38-42/43

          Harmful by inhalation.
          Irritating to eyes, respiratory system and skin.
          May cause sensitization by inhalation and skin contact.
          S PhraseS: 26-36

          In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
          Wear suitable protective clothing.
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Blue Ice Only
          Toxicity Harmful
          Storage +2°C to +8°C
          Do not freeze Yes
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          SDS

          Title

          Safety Data Sheet (SDS) 

          Certificates of Analysis

          TitleLot Number
          480716

          References

          Reference overview
          Means, R.E. and Desrosiers, R.C. 2000. J. Virol. 74, 11181.
          Prime, S., et al. 1996. J. Chromatogr. A. 720, 263.
          Roggentin, P., et al. 1995. Biol. Chem. Hoppe Seyler 376, 569
          Dwek, R.A., et al. 1993. Annu. Rev. Biochem. 62, 65.
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision17-September-2007 RFH
          SynonymsN-Acetylneuraminyl Hydrolase, Sialidase
          DescriptionRecombinant, Arthrobacter ureafaciens α2-3,6,8,9-Neuraminidase expressed in E. coli. Catalyzes the hydrolysis of all non-reducing terminal sialic acid residues from complex carbohydrates and glycoproteins. High concentrations of this sialidase and prolonged periods of incubation result in the additional cleavage of branched sialic acids. This enzyme is found as two active species of MW 60000 and 69000.
          FormLiquid
          FormulationIn 20 mM Tris-HCl, 20 mM NaCl, pH 7.5.
          Recommended reaction conditions50 mM sodium phosphate buffer, pH 6.0.
          CAS number9001-67-6
          RTECSQQ3450000
          EC number3.2.1.18
          ContaminantsN-acetylglucosaminidase, α-fucosidase, α- and β-galactosidase, α- and β-mannosidase, proteases: none detected.
          Specific activity≥40 units/mg protein
          Activity≥5 units/ml
          Unit definitionOne unit is defined as the amount of enzyme that will catalyze the release of 1 µmol of methylumbelliferone from 2ʹ-(4-methylumbelliferyl)-α-D-N-acetylneuraminic acid per min at 37°C, pH 5.0. (Note: 1 mU = 1 milliunit).
          Storage +2°C to +8°C
          Do Not Freeze Yes
          Toxicity Harmful
          ReferencesMeans, R.E. and Desrosiers, R.C. 2000. J. Virol. 74, 11181.
          Prime, S., et al. 1996. J. Chromatogr. A. 720, 263.
          Roggentin, P., et al. 1995. Biol. Chem. Hoppe Seyler 376, 569
          Dwek, R.A., et al. 1993. Annu. Rev. Biochem. 62, 65.