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SCR005 | Accutase cell detachment solution

SCR005
100 mL  
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      Overview

      Replacement Information
      Description
      Catalogue NumberSCR005
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAccutase cell detachment solution
      OverviewA cell detachment solution of proteolytic and collagenolytic enzymes. Useful for the routine detachment of cells from standard tissue culture plasticware and adhesion coated plasticware, including SmartPlastic™. ACCUTASE™ does not contain mammalian or bacterial derived products.

      Because the reagent does not contain universally proteolytic enzymes it may not release all cell/tissue types uniformly. Some optimization of cell type and passage number, as well as, incubation time and reagent strength typically needs to be performed to optimize the reagents effectiveness.

      ACCUTASE™ has been shown to be effective on a wide variety of cell types including: fibroblasts, keratinocytes, vascular endothelial cells, hepatocytes, vascular smooth muscle cells, hepatocyte progenitors, primary chick embryo neuronal cells, bone marrow stem cells, adherent CHO and BHK cells, macrophages, 293 cells, L929 cells, immortalized mouse testicular germ cells, 3T3, Vero, COS, HeLa, NT2, MG63, M24 and A375 metastatic melanoma, gliomas U251 and D54, HT1080 fibrosarcoma cells, and Sf9 insect cells.

      Each lot of ACCUTASE™ is tested for Sterility (by USP membrane filtration method), enzymatic activity (tested with synthetic chromagenic tetrapeptides) and cell detachment from tissue culture plastic.
      References
      Product Information
      Presentation100 ml, ready to use, frozen sterile liquid. 1X ACCUTASE enzymes in Dulbecco's PBS (0.2 g/L KCl, 0.2 g/L KH2PO4, 8 g/L NaCl, and 1.15 g/L Na2HPO4) containing 0.5 mM EDTA·4Na and 3 mg/L Phenol Red.
      Applications
      ApplicationA cell detachment solution of proteolytic & collagenolytic enzymes. The reagent is useful for creating single cell suspensions from clumped cell cultures for accurate cell counting, detachment of cells from primary tissue.
      Application NotesCell Detachment

      1. Thaw ACCUTASE™ at room temperature.

      2. Wash plate, flask or beads with sterile PBS.

      3. Add ACCUTASE™ to culture dish or flask using aseptic procedures at 10 ml per 75-cm2 surface area.

      4. Return culture to 37°C incubator and allow cells to detach (5-10 minutes).

      5. Count cells and passage as usual. No additional washes or enzyme inhibitors are required



      Optimal incubation times and reagent strength needs to be determined for each cell/tissue type tested by the enduser.
      Biological Information
      Media FormLiquid
      Stem Cell Type
      • Mouse Embryonic Stem Cells
      • Human Embryonic Stem Cells
      • Mesenchymal Stem Cells
      • Neural Stem Cells
      • Hematopoietic Stem Cells
      • Epithelial Cells
      • Pancreatic Stem Cells
      • Cardiac Stem Cells
      • Induced Pluripotent Stem Cells
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable when stored at -20°C. Refer to lot expiration date. Recommended storage upon receipt is -20°C. After thawing, ACCUTASE may be stored for up to 2 months at 4°C. DO NOT STORE AT ROOM TEMP
      Packaging Information
      Material Size100 mL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      ACCUTASE SOLUTION - 1970316 1970316
      ACCUTASE SOLUTION - 2000796 2000796
      ACCUTASE SOLUTION - 2022641 2022641
      ACCUTASE SOLUTION - 2058324 2058324
      ACCUTASE SOLUTION - 2092639 2092639
      ACCUTASE SOLUTION - 2138544 2138544
      ACCUTASE SOLUTION - 2197143 2197143
      ACCUTASE™ SOLUTION 2441885
      ACCUTASE™ SOLUTION 2850698
      ACCUTASE™ SOLUTION - 2464529 2464529

      References

      Reference overviewPub Med ID
      HDAC1 regulates pluripotency and lineage specific transcriptional networks in embryonic and trophoblast stem cells.
      Kidder B. L. & Palmer S.
      Nucleic Acids Res.  1-15  2011

      Show Abstract
      22156375 22156375

      Brochure

      Title
      Murine Embryonic Stem Cell Culture

      Technical Info

      Title
      Derivation of oligodendrocyte progenitor cells from a human neural stem cell line

      Data Sheet

      Title
      ACCUTASE™ SOLUTION
      Reprogramming Cell Fate and Function Novel Strategies for iPSC Generation, Characterization, and Differentiation

      FAQ

      QuestionAnswer
      What is the difference between Accuma and Accutase? Accumax (Catalog #SCR006) will dissociate clumped cells in minutes resulting in a single cell suspension. It will yield accurate reproducible cell counts, is cost effective and is ready to use. The Accutase (Catalog #SCR005) contains phenol red. It will detect adherent cells within minutes and will dissociate tissues for primary cell culture use. It also will yield accurate reproducible cell counts, is cost effective and is ready to use.

      Newsletters / Publications

      Title
      Cellutions - The newsletter for Cell Biology Researchers Volume 3: 2011

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      Product Families

      Categories

      Life Science Research > Cell Culture and Systems > Cell Dissociation Reagents