SCR004 | Alkaline Phosphatase Detection Kit

Staining with Alkaline Phosphatase Detection Kit

(A) (B) (C)

Alkaline Phosphatase staining of ES cells. High magnification revealed (A) Undifferentiated ES cells (mouse MBL.5 cell line) - cultured for five days in media containing CHEMICON's LIF/ESGRO®. A concentration of 103 Units/mL is used for inhibition of differentiation. (B) Differentiated ES cells - cultured at low-medium density for three days in media without any LIF/ESGRO®. (C) Differentiated ES cells - cultured at low-medium density for six days in media without any LIF/ESGRO®.
Staining with Alkaline Phosphatase Detection Kit

(A) (B) (C)

100 assays  
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      Replacement Information

      Key Specifications Table

      Key Applications
      Catalogue Number SCR004
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      Description Alkaline Phosphatase Detection Kit
      Overview Millipore's Alkaline Phosphatase Detection Kit (Catalog number SCR004) is a specific and sensitive tool for the phenotypic assessment of ES cell differentiation by the determination of AP activity.
      Materials Required but Not Delivered 1. Fixative (e.g. 4% Paraformaldehyde in PBS)

      2. 1 x Rinse Buffer (e.g. TBST: 20mM Tris-HCl, pH 7.4, 0.15M NaCl, 0.05% Tween-20)

      3. Hematoxylin (optional)

      4. Microscope
      Product Information
      • Fast Red Violet solution (0.8g/L stock) (Part No. 90239). Two 15mL bottles.
      • Napthol AS-BI phosphate solution (4mg/mL) in AMPD buffer (2mol/L), pH 9.5 (Part No. 90234). One 15mL bottle.
      Application This Alkaline Phosphatase Detection Kit is a specific & sensitive tool for the phenotypic assessment of Embryonic Stem (ES) cell differentiation by the determination of AP activity.
      Key Applications
      • Stem Cell Culture
      Application Notes Embryonic stem (ES) cells are totipotent cells derived from the inner cell mass (ICM) of preimplantation mammalian embryos and are capable of unlimited, undifferentiated proliferation in vitro [1,2,3]. Undifferentiated murine ES cells can be maintained in vitro for extended periods in media containing the cytokine, leukemia-inhibitory factor (LIF) or Millipore's proprietary ES cell culture reagent, ESGRO [4,5]. The undifferentiated state of ES cells can be characterized by high level of expression of Alkaline Phosphatase (AP) [6], the expression of surface markers including SSEA and TRA antigens and the transcription factor Oct-4.

      Millipore's Alkaline Phosphatase Detection Kit (Catalog number SCR004) is a specific and sensitive tool for the phenotypic assessment of ES cell differentiation by the determination of AP activity.

      Available separately from Millipore are the monoclonal antibodies TRA-2-49 (Catalog Number MAB4349) and TRA-2-54 (Catalog Number MAB4354), which permit the detection of Liver / Bone / Kidney isozyme of AP as expressed by ES cells. When used in flow cytometry, these reagents permit a quantitative assessment of AP expression by ES cells [7].

      For Research Use Only; Not for use in diagnostic procedures
      Biological Information
      Species Reactivity
      • Mammals
      Stem Cell Type
      • Mouse Embryonic Stem Cells
      • Induced Pluripotent Stem Cells
      • Cardiac Stem Cells
      • Pancreatic Stem Cells
      • Epithelial Cells
      • Hematopoietic Stem Cells
      • Neural Stem Cells
      • Mesenchymal Stem Cells
      • Human Embryonic Stem Cells
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions The Alkaline Phosphatase Detection Kit consists of two components used for determining AP activity.

      When stored at 2-8º C, the kit components are stable up to the expiration date. Do not freeze or expose to elevated temperatures. Discard any remaining reagents after the expiration date.
      Packaging Information
      Material Size 100 assays
      Transport Information
      Supplemental Information




      Safety Data Sheet (SDS)  

      References | 18 Available | See All References

      Reference overviewApplicationPub Med ID
      Establishment of LIF-dependent human iPS cells closely related to basic FGF-dependent authentic iPS cells.
      Hiroyuki Hirai,Meri Firpo,Nobuaki Kikyo
      PloS one 7 2012

      Show Abstract
      BMP4 Signaling Acts via dual-specificity phosphatase 9 to control ERK activity in mouse embryonic stem cells.
      Zhongwei Li,Teng Fei,Jianping Zhang,Gaoyang Zhu,Lu Wang,Danyu Lu,Xiaochun Chi,Yan Teng,Ning Hou,Xiao Yang,Hongquan Zhang,Jing-Dong J Han,Ye-Guang Chen
      Cell stem cell 10 2012

      Show Abstract
      Efficient iPS cell production with the MyoD transactivation domain in serum-free culture.
      Hiroyuki Hirai,Nobuko Katoku-Kikyo,Peter Karian,Meri Firpo,Nobuaki Kikyo
      PloS one 7 2012

      Show Abstract
      Radical acceleration of nuclear reprogramming by chromatin remodeling with the transactivation domain of MyoD.
      Hirai, Hiroyuki, et al.
      Stem Cells, 29: 1349-61 (2011) 2011

      Show Abstract
      Embryonic stem cells require Wnt proteins to prevent differentiation to epiblast stem cells.
      Derk ten Berge,Dorota Kurek,Tim Blauwkamp,Wouter Koole,Alex Maas,Elif Eroglu,Ronald K Siu,Roel Nusse
      Nature cell biology 13 2011

      Show Abstract
      Large scale phosphoproteome profiles comprehensive features of mouse embryonic stem cells.
      Li QR, Xing XB, Chen TT, Li RX, Dai J, Sheng QH, Xin SM, Zhu LL, Jin Y, Pei G, Kang JH, Li YX, Zeng R
      Molecular & cellular proteomics : MCP 10 M110.001750. Epub 2010 Dec 13. 2011

      Show Abstract
      Establishment of induced pluripotent stem cells from aged mice using bone marrow-derived myeloid cells.
      Zhao Cheng,Sachiko Ito,Naomi Nishio,Hengyi Xiao,Rong Zhang,Haruhiko Suzuki,Yayoi Okawa,Toyoaki Murohara,Ken-Ichi Isobe
      Journal of molecular cell biology 3 2011

      Show Abstract
      Efficient feeder-free episomal reprogramming with small molecules.
      Junying Yu,Kevin Fongching Chau,Maxim A Vodyanik,Jinlan Jiang,Yong Jiang
      PloS one 6 2011

      Show Abstract Full Text Article
      Ethanol, acetaldehyde, and estradiol affect growth and differentiation of rhesus monkey embryonic stem cells.
      Catherine A VandeVoort,Dana L Hill,Charles L Chaffin,Alan J Conley
      Alcoholism, clinical and experimental research 35 2011

      Show Abstract
      Epigenetic regulation of Nanog expression by Ezh2 in pluripotent stem cells.
      Aranzazu Villasante,Daniela Piazzolla,Han Li,Gonzalo Gomez-Lopez,Malek Djabali,Manuel Serrano
      Cell cycle (Georgetown, Tex.) 10 2011

      Show Abstract


      Human Stem Cell Systems
      Murine Embryonic Stem Cell Culture

      Technical Info

      A Comparative Analysis of Human Embryonic Stem Cells Cultured in a Variety of Media Conditions
      Accumax Dissociation Reagent for the Serial Passaging of Human Embryonic Stem Cells
      Collagenase Type I for Enzymatic Passaging of Human Embryonic Stem Cells in HEScGRO Medium
      Millipore Tools for Characterizing Induced Pluripotent Stem Cells
      OsteoMAX-XF™: A Novel Serum-free and Xeno-free Differentiation Medium for Accelerated Osteogenic Differentiation of Human Mesenchymal Stem Cells
      Single transfection of a synthetic polycistronic self-replicative RNA yields high numbers of transgene-free human iPSCs

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      Alkaline Phosphatase Detection Kit