PMEF-CF | EmbryoMax® Primary Mouse Embryo Fibroblasts, Strain CF1, Mytomycin C treated, passage 3

PMEF-CF
5 vials  5-6x106 ea
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      Overview

      Replacement Information

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      Key Specifications Table

      Key Applications
      Cult
      Description
      Catalogue NumberPMEF-CF
      Trade Name
      • Embryomax
      • Specialty Media
      DescriptionEmbryoMax® Primary Mouse Embryo Fibroblasts, Strain CF1, Mytomycin C treated, passage 3
      OverviewPrimary Mouse Embryo Fibroblasts, Strain CF1, are Mytomycin C treated and serve as a feeder layer for both mouse and human ES/IPS cells.

      Plating MEF Feeder Cells

      Procedure:

      1. Prior to thawing PMEF feeder cells, coat plates/flasks with Gelatin solution.
      2. Thaw PMEF vial(s) quickly in a 37 °C water bath and transfer to a 15 mL tube (already containing 10 mL of warm PMEF Feeder Cell Medium). Gently invert the tube to distribute, and centrifuge at 300 xg for 4–5 minutes.
      3. Remove supernatant and resuspend the cell pellet in warm PMEF Feeder Cell Medium.
      4. Remove the Gelatin solution from plates/flasks, and aliquot the PMEF feeder cell suspension at the densities recommended in Table 4.1 of the mouse ES protocol guide
      5. Incubate the PMEF Feeder cells at 37 °C with 5% CO2. Use Figures 4A, B and C in the mouse ES protocol guide as a guide for an estimate of correct PMEF density and
      appearance. Gelatinized plates may be used for 12–14 days.
      Alternate Names
      • Non drug resistant PMEFs from CF-1 mice- Mitomycin C treated, CF-1 PMEFs, CF-1 MEFs, MEFs, mouse feeder cells
      Background InformationThe EmbryoMax range of PMEF cells provides researchers with a convenient solution for ES cell culture by eliminating the need for time consuming feeder cell isolation and preparation. Many embryonic stem cell culture protocols necessitate the use of primary mouse embryo fibroblast (PMEF) cells. In these protocols, ES cells are typically cultured on a monolayer of PMEF feeder cells. Feeder cells perform two important roles in stem cell culture: they secrete several important growth factors into the medium, which help maintain pluripotency, and they provide a cellular matrix for ES cells to grow.
      References
      Product Information
      Applications
      ApplicationPrimary Mouse Embryo Fibroblasts, Strain CF1, are Mytomycin C treated and serve as a feeder layer for both mouse and human ES/IPS cells.
      Key Applications
      • Stem Cell Culture
      Biological Information
      Stem Cell Type
      • Human Embryonic Stem Cells
      • Mouse Embryonic Stem Cells
      • PMEFs
      • Induced Pluripotent Stem Cells
      Cell Line Type
      • Primary Mouse Embryo Fibroblasts
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsAfter receipt, vials should be stored at -80°C. If storing for longer than 6 months, store in the vapor phase of liquid nitrogen (make sure the cap is tight) for up to 2 years.
      Packaging Information
      Material Size5 vials
      Material Package5-6x106 ea
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Protocols

      Title
      Plating PMEF Feeder Cells Protocol

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      EmbryoMax#174; PMEF Cells Strain CF-1, Mito-C Treated - 2040624-1 2040624-1
      EmbryoMax#174; PMEF Cells Strain CF-1, Mito-C Treated - 2048176-1 2048176-1
      EmbryoMax#174; PMEF Cells Strain CF-1, Mito-C Treated - 2010171-1 2010171-1
      EmbryoMax#174; PMEF Cells Strain CF-1, Mito-C Treated - 2043018-1 2043018-1
      EmbryoMax#174; PMEF Cells Strain CF-1, Mito-C Treated - 2077214-1 2077214-1
      EmbryoMax#174; PMEF Cells Strain CF-1, Mito-C Treated - 2116040-1 2116040-1
      EmbryoMax#174; PMEF Cells Strain CF-1, Mito-C Treated - 01030-4 01030-4
      EmbryoMax#174; PMEF Cells Strain CF-1, Mito-C Treated - 10226-4 10226-4
      EmbryoMax#174; PMEF Cells Strain CF-1, Mito-C Treated - 10226-5 10226-5
      EmbryoMax#174; PMEF Cells Strain CF-1, Mito-C Treated - 10226-6 10226-6

      References

      Reference overviewPub Med ID
      Transcriptional signature and memory retention of human-induced pluripotent stem cells.
      Marchetto, Maria C N, et al.
      PLoS ONE, 4: e7076 (2009)  2009

      Show Abstract
      19763270 19763270
      Establishment of rat embryonic stem cells and making of chimera rats.
      Ueda S. et al.
      PLoS One  3(7)  e2800  2008

      Show Abstract
      18665239 18665239

      Brochure

      Title
      Murine Embryonic Stem Cell Culture

      Technical Info

      Title
      A Comparative Analysis of Human Embryonic Stem Cells Cultured in a Variety of Media Conditions
      Accumax Dissociation Reagent for the Serial Passaging of Human Embryonic Stem Cells
      Single transfection of a synthetic polycistronic self-replicative RNA yields high numbers of transgene-free human iPSCs
      The Genetically Modified Mouse: An Indispensable Tool for Disease Modeling (EMD)