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MAB4401 | Anti-Oct-4 Antibody, clone 10H11.2

MAB4401
100 µg  
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      Overview

      Replacement Information

      Key Specifications Table

      Species Reactivity Key Applications Host Format Antibody Type
      H WB, FC, ICC, ELISA M Purified Monoclonal Antibody
      Description
      Catalogue Number MAB4401
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      Description Anti-Oct-4 Antibody, clone 10H11.2
      Overview Purified monoclonal antibody for the detection of Human Oct-4.
      Alternate Names
      • Octamer-binding transcription factor 3
      • POU class 5 homeobox 1
      • POU domain class 5, transcription factor 1
      • POU domain, class 5, transcription factor 1
      • POU-type homeodomain-containing DNA-binding protein
      • octamer-binding transcription factor-3
      Background Information Octamer-4 (Oct-4), a member of the POU family of transcription factors, has been demonstrated to be vital for the formation of self-renewing pluripotent stem cells. During embryogenesis expression of Oct-4 is limited to pluripotent cells of the inner cell mass (ICM) that contribute to the formation of all fetal cell types. This relationship between Oct-4 and pluripotency has seen this transcription factor emerge as a marker of pluripotent stem cells. Undifferentiated human and murine pluripotent Embryonic Stem (ES) and Embryonic Carcinoma (EC) cells express Oct-4. Additionally, murine Embryonic Germ (EG) cells are also known to express Oct-4. Following stem cell differentiation, the level of Oct-4 expression decreases. Oct-4 expression by human EG cells is not known.
      References
      Product Information
      Format Purified
      Control
      • Human embryonic stem cell lysate.
      Presentation Purified Ascites presented as a liquid in PBS with 0.1% sodium azide.
      Applications
      Application Detect Oct-4 using this Anti-Oct-4 Antibody, clone 10H11.2 validated for use in WB, FC, IC, ELISA.
      Key Applications
      • Western Blotting
      • Flow Cytometry
      • Immunocytochemistry
      • ELISA
      Application Notes Flow Cytometry: A previous lot of this antibody was used in FC.

      ELISA: A previous lot of this antibody was used in ELISA.

      Immunocytochemistry: A previous lot of this antibody was used in IC. H9 cells in culture on a mouse embronic fibroblast feeder layer were labeled by a standard indirect IF protocol. Oct-4 labelling in green, DAPI in blue, & SSEA-3 (MAB4303 in red).

      Optimal working dilutions must be determined by the end user.
      Biological Information
      Immunogen Recombinant GST fusion protein, human Oct-4 (aa 1-134).
      Clone clone 10H11.2
      Concentration Please refer to the Certificate of Analysis for the lot-specific concentration.
      Host Mouse
      Specificity Reacts with human Oct-4 only, and detects the Oct-4A isoform. (Note: Please order Catalog No. MAB4419 for use with mouse embryonic stem cells).
      Isotype IgG1
      Species Reactivity
      • Human
      Species Reactivity Note The clone reacts strongly with specific nuclear binding to human ES cells and cross-reacts weakly with mouse ES cells.
      Antibody Type Monoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • POU5F1
      • Oct-3
      • MGC22487
      • Oct4
      • OTF4
      • OTF3
      • OCT3
      • OCT4
      • Oct-4
      Purification Method Protein A Purfied
      UniProt Number
      UniProt Summary FUNCTION: SwissProt: Q01860 # Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3'). Prime candidate for an early developmental control gene.
      SIZE: 360 amino acids; 38571 Da
      SUBCELLULAR LOCATION: Nucleus.
      TISSUE SPECIFICITY: Ubiquitous. Expressed at low levels.
      SIMILARITY: SwissProt: Q01860 ## Belongs to the POU transcription factor family. Class- 5 subfamily. & Contains 1 homeobox DNA-binding domain. & Contains 1 POU-specific domain.
      MISCELLANEOUS: Several pseudogenes of POU5F1 have been described on chromosomes 1, 3, 8, 10 and 12. 2 of them, localized in chromosomes 8 and 10, are transcribed in cancer tissues but not in normal ones and may be involved in the regulation of POU5F1 gene activity in carcinogenesis.
      Stem Cell Type
      • Human Embryonic Stem Cells
      • Induced Pluripotent Stem Cells
      Molecular Weight 44 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assurance Evaluated by western blot on mouse P1 brain lysates.

      Western Blotting: 1:500 dilution of this antibody detected Oct-4 on 10 µg of mouse P1 brain lysates
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions Stable for 1 year at from date of receipt.
      Packaging Information
      Material Size 100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-Oct-4, clone 10H11.2 2474968
      Anti-Oct-4, clone 10H11.2 - 2392284 2392284
      Anti-Oct-4, clone 10H11.2 - 1991253 1991253
      Anti-Oct-4, clone 10H11.2 - 2066310 2066310
      Anti-Oct-4, clone 10H11.2 - LV1679970 LV1679970
      Anti-Oct-4, clone 10H11.2 - LV1786447 LV1786447
      Anti-Oct-4, clone 10H11.2 - NG1865606 NG1865606
      Anti-Oct-4, clone 10H11.2 - NG1927045 NG1927045
      Anti-Oct-4, clone 10H11.2 - PSO1553824 PSO1553824
      Anti-Oct-4, clone 10H11.2 -2512486 2512486
      Anti-Oct-4, clone 10H11.2 -2580657 2580657
      Anti-Oct-4, clone 10H11.2 -2610382 2610382
      Anti-Oct-4, clone 10H11.2 -2683913 2683913
      Anti-Oct-4, clone 10H11.2 -2697574 2697574
      Anti-Oct-4, clone 10H11.2 -2727265 2727265

      References | 18 Available | See All References

      Reference overviewApplicationPub Med ID
      Transcriptional profiling of ectoderm specification to keratinocyte fate in human embryonic stem cells.
      Tadeu, AM; Lin, S; Hou, L; Chung, L; Zhong, M; Zhao, H; Horsley, V
      PloS one 10 e0122493 2015

      Show Abstract
      25849374 25849374
      Progerin expression disrupts critical adult stem cell functions involved in tissue repair.
      Pacheco, LM; Gomez, LA; Dias, J; Ziebarth, NM; Howard, GA; Schiller, PC
      Aging 6 1049-63 2014

      Show Abstract
      Western Blotting25567453 25567453
      Metabolic profiling and flux analysis of MEL-2 human embryonic stem cells during exponential growth at physiological and atmospheric oxygen concentrations.
      Turner, J; Quek, LE; Titmarsh, D; Krömer, JO; Kao, LP; Nielsen, L; Wolvetang, E; Cooper-White, J
      PloS one 9 e112757 2014

      Show Abstract
      Western Blotting25412279 25412279
      Generation of iPS Cells from Human Hair Follice Dermal Papilla Cells.
      Muchkaeva, IA; Dashinimaev, EB; Artyuhov, AS; Myagkova, EP; Vorotelyak, EA; Yegorov, YY; Vishnyakova, KS; Kravchenko, IE; Chumakov, PM; Terskikh, VV; Vasiliev, AV
      Acta naturae 6 45-53 2014

      Show Abstract
      24772326 24772326
      Characterization of embryonic stem-like cells derived from HEK293T cells through miR302/367 expression and their potentiality to differentiate into germ-like cells.
      Wang, L; Zhu, H; Wu, J; Li, N; Hua, J
      Cytotechnology 66 729-40 2014

      Show Abstract
      24091881 24091881
      Identification of rabbit annulus fibrosus-derived stem cells.
      Liu, C; Guo, Q; Li, J; Wang, S; Wang, Y; Li, B; Yang, H
      PloS one 9 e108239 2014

      Show Abstract
      25259600 25259600
      Synergistic effect of trichostatin A and scriptaid on the development of cloned rabbit embryos.
      Chen, CH; Du, F; Xu, J; Chang, WF; Liu, CC; Su, HY; Lin, TA; Ju, JC; Cheng, WT; Wu, SC; Chen, YE; Sung, LY
      Theriogenology 79 1284-93 2013

      Show Abstract
      23566670 23566670
      High-content imaging-based screening of microenvironment-induced changes to stem cells.
      Vega, SL; Liu, E; Patel, PJ; Kulesa, AB; Carlson, AL; Ma, Y; Becker, ML; Moghe, PV
      Journal of biomolecular screening 17 1151-62 2012

      Show Abstract
      22811477 22811477
      Dynamic profiles of Oct-4, Cdx-2 and acetylated H4K5 in in-vivo-derived rabbit embryos.
      Chen, CH; Xu, J; Chang, WF; Liu, CC; Su, HY; Chen, YE; Du, F; Sung, LY
      Reproductive biomedicine online 25 358-70 2012

      Show Abstract
      22877942 22877942
      Spatial and temporal distribution of Oct-4 and acetylated H4K5 in rabbit embryos.
      Chen, CH; Chang, WF; Liu, CC; Su, HY; Shyue, SK; Cheng, WT; Chen, YE; Wu, SC; Du, F; Sung, LY; Xu, J
      Reproductive biomedicine online 24 433-42 2012

      Show Abstract
      22381206 22381206

      Brochure

      Title
      Human Stem Cell Systems

      Technical Info

      Title
      A Comparative Analysis of Human Embryonic Stem Cells Cultured in a Variety of Media Conditions
      Accumax Dissociation Reagent for the Serial Passaging of Human Embryonic Stem Cells
      Single transfection of a synthetic polycistronic self-replicative RNA yields high numbers of transgene-free human iPSCs

      Data Sheet

      Title
      Anti-Oct-4, clone 10H11.2 - Data Sheet
      STEMCCA Lentivirus Reprogramming Kits

      Posters

      Title
      Efficient generation of transgene-free human and mouse iPS cells using a cell-permeant Tat-Cre protein

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      Alternative Packsize

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      MAB4401-KC Anti-Oct-4, clone 10H11.2 (1mg) KC Show Pricing & Availability

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies