Amicon Ultra-2 mL Centrifugal Filters for DNA and Protein Purification and Concentration

With their vertical membrane design, Amicon Ultra 2 mL filters deliver great performance and lower spin times

Overview

Specifications

Ordering Information

Amicon Ultra-2 mL Centrifugal Filters for Protein Purification and Concentration Clear Sorting & Filtering
Catalog Numbericon Descriptionicon Pack Sizeicon
UFC200324Amicon Ultra-2 Centrifugal Filter Unit with Ultracel-3 membrane24 Show Pricing & Availability
UFC201024Amicon Ultra-2 Centrifugal Filter Unit with Ultracel-10 membrane24 Show Pricing & Availability
UFC203024Amicon Ultra-2 Centrifugal Filter Unit with Ultracel-30 membrane24 Show Pricing & Availability
UFC205024Amicon Ultra-2 Centrifugal Filter Unit with Ultracel-50 membrane24 Show Pricing & Availability
UFC210024Amicon Ultra-2 Centrifugal Filter Unit with Ultracel-100 membrane24 Show Pricing & Availability

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    C86533With their vertical membrane design, Amicon Ultra 2 mL filters deliver great performance and lower spin times

    • Concentrates 2 mL down to 15-70 µl
    • 98% sample recovery
    • Fast processing time - 10 to 30 minute spin times
    • Engineered dead stop
    • Reverse spin capability provides consistent recoveries

    Applications

    • Concentration of biological samples containing antigens, antibodies, enzymes, nucleic acids, or microorganisms
    • Purification of macromolecular components found in tissue culture extracts or cell lysates
    • Primer removal, PCR cleanup, removal of linkers or macromolecular labels from a reaction mix, and protein removal prior to HPLC
    • Desalting, buffer exchange and protein dialysis
    • Size exclusion and protein fractionation

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    Material Supplied

    The Amicon Ultra-2 device is supplied with two microcentrifuge tubes. During operation, one tube is used to collect filtrate; the other tube is to recover the concentrated sample.

    Performance – Protein Concentration

    Spin conditions: Swinging bucket rotor, 4,000 × g, room temperature, 2 mL starting volume.<br />Protein markers used: Cytochrome c for 3K and 10K, BSA for 30K and 50K, and IgG for 100K, n=4.

    Spin conditions: Swinging bucket rotor, 4,000 × g, room temperature, 2 mL starting volume.
    Protein markers used: Cytochrome c for 3K and 10K, BSA for 30K and 50K, and IgG for 100K, n=4.

    Spin conditions: 35° fixed angle rotor, 7,500 × g, room temperature, 2 mL starting volume.<br />Protein markers used: Cytochrome c for 3K and 10K, BSA for 30K and 50K, and IgG for 100K, n=4.

    Spin conditions: 35° fixed angle rotor, 7,500 × g, room temperature, 2 mL starting volume.
    Protein markers used: Cytochrome c for 3K and 10K, BSA for 30K and 50K, and IgG for 100K, n=4.

    Performance – DNA Concentration

    The Amicon Ultra-2 30K device provides the best balance between PCR recovery and PCR primer removal for double-stranded DNA for base pairs ranging from 137 to 1159.

    Swinging Bucket Rotor 35° Fixed Angle Rotor
    Amicon Ultra
    Device
    PCR Product
    (base pairs)
    PCR Primer (bases) PCR Recovery
    (%)
    PCR Primer
    Removal (%)
    Final Volume
    (μL)
    PCR Recovery (%) PCR Primer
    Removal (%)
    Final Volume
    (μL)
    30K 137 10 92 100 37 93 100 27
    20 94 93 39 94 95 31
    48 95 71 36 93 78 31
    100K 137 10 60 100 33 56 100 16
    20 50 100 33 41 100 17
    48 50 92 35 39 91 16
    Spin conditions: Swinging bucket rotor, 4,000 x g for 40 min; fixed angle rotor, 7,500 x g for 15 min; room temperature, 100 μL PCR diluted to 2,000 μL starting volume, n=3.

    Protein Retention and Concentrate Recovery

    Typical Retention of Protein Markers
    Marker
    (Concentration)
    Molecular
    Weight
    Device
    NMWL
    % Retention
    Swinging Bucket
    Spin Time
    (min)
    % Retention
    Fixed Angle
    Spin Time
    (min)
    Cytochrome c (0.25 mg/mL) 12,400 3K > 99 60 > 99 60
    Cytochrome c (0.25 mg/mL) 12,400 10K > 98 40 > 99 20
    BSA (1 mg/mL) 67,000 30K > 99 20 > 99 15
    BSA (1 mg/mL) 67,000 50K > 96 15 > 97 10
    IgG (1 mg/mL) 156,000 100K > 98 30 > 98 20
    Spin Conditions: Swinging bucket rotor, 4,000 x g, or 35º fixed angle rotor, 7,500 x g, 2 mL starting volume, room temperature, n=4.

    Desalting or Diafiltration

    Desalting, buffer exchange or diafiltration are important methods for removing salts or solvents in solutions containing biomolecules. The removal of salts or the exchange of buffers can be accomplished in the Amicon Ultra-2 device by concentrating the sample, then reconstituting the concentrate to the original sample volume with any desired solvent. The process of "washing out" can be repeated until the concentration of the contaminating microsolute has been sufficiently reduced. See example below.

    Volumes
    Maximum initial sample volume, µL 2.0
    Typical final concentration volume, µL 15 – 70
    OPTIMAL and maximum relative centrifugal force – Swinging bucket rotor 4,000 x g for concentration spin
    1,000 x g for recovery spin
    OPTIMAL and maximum relative centrifugal force – Fixed angle rotor 7,500 x g for concentration spin
    1,000 x g for recovery spin
    Active membrane area, cm2 1
    Hold up volume, µL < 5
    Materials
    Filter device Copolymer styrene/butadiene
    Membrane Millipore Ultracell low-binding regenerated cellulose
    Collection tube Polypropylene
    The ultrafiltration membranes in Amicon Ultra-2 mL devices contain trace amounts of the following: Amicon Ultra 3K, 10K, 30K, 50K, 100K devices: glycerine
    If these materials interfere with analysis, pre-rinse the device with buffer or Milli-Q water. If interference continues, rinse with 0.1 N NaOH followed by a second spin of buffer or Milli-Q water.
    Dimensions
    Filter device, mm (in.) Diameter: 15.9 (0.63)
    Length: 70.7 (2.78)
    Tube, mm (in.) Diameter: 13.8 (0.54)
    Length: 52.9 (2.08)
    Filter device and tube, mm (in.) Length (concentration mode; device in tube): 13.7 (0.54)
    Length (reverse spin; device upside down in tube): 34.5 (1.36)