Scepter™ 2.0 Handheld Automated Cell Counter

Accurate cell counts in 30 seconds

Overview

Specifications

Ordering Information

Cell Counter Clear Sorting & Filtering
Catalog Numbericon Descriptionicon Pack Sizeicon
PHCC20040Scepter 2.0 Handheld Automated Cell Counter, w/ 50pk of 40μM Sensors1 ea Show Pricing & Availability
PHCC20060Scepter 2.0 Handheld Automated Cell Counter, w/ 50pk of 60μM Sensors1 ea Show Pricing & Availability

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Cell Counter Sensors & Accessories Clear Sorting & Filtering
Catalog Numbericon Descriptionicon Cell Size Range, µmicon Pack Sizeicon
PHCC0CLIPScepter O-ring Kit1 kit Show Pricing & Availability
PHCC40050Scepter Cell Counter Sensors, 40 µm - Qty: 504–1250/pk Show Pricing & Availability
PHCC40500Scepter Cell Counter Sensors, 40 µm - Qty: 5004–12500 (10 x 50/pk) Show Pricing & Availability
PHCC60050Scepter Cell Counter Sensors, 60 µm - Qty: 508–2550/pk Show Pricing & Availability
PHCC60500Scepter Cell Counter Sensors, 60 µm - Qty: 5008–25500 (10 x 50/pk) Show Pricing & Availability
PHCCBEADSScepter Test Bead Vial1 ea Show Pricing & Availability
PHCCCABLEScepter USB Cable1 ea Show Pricing & Availability
PHCCP0WERUniversal Power Adapter1 ea Show Pricing & Availability

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    Documentation

    Brochure

    Title
    Advanced cell culture systems for every challenge.
    Scepter™ 2.0 Cell Counter

    Technical Info

    Title
    Counting Yeast Cells for Brewing and Wine Industries Can Be Facilitated by the Scepter™ 2.0 Handheld Automated Cell Counter
    Precise and Accurate Bead Counting Using the Scepter™ 2.0 Handheld Automated Cell Counter
    Rapid Assessment of Size Changes Using the Scepter Cell Counter can be Predictive of Cell Death
    Rapid Counting of Somatic Cells in Dairy Milk Using the Scepter 2.0 Cell Counter, Following Spin-Wash Sample Preparation

    User Guides

    Title
    Scepter Handheld Automated Cell Counter Declaration of Conformity
    Scepter Handheld Automated Cell Counter Overview
    Scepter Handheld Automated Cell Counter User Guide
    Scepter Sensor Correct Orientation Insert
    Scepter Software Pro Quick Guide
    Scepter Software Pro User Guide

    C85360Accurate cell counts in 30 seconds

    For more detailed Scepter information, visit the Scepter Technology webpage.

    The Scepter 2.0 handheld automated cell counter provides a fast and convenient method for counting cells and particles and is compatible with both the 40 and 60uM sensor. The system employs the Coulter principle in a miniaturized, handheld, format enabling rapid cell counting; what used to take 10 minutes now takes less than 30 seconds. The user prepares a dilution of the cell culture of interest and uses the Scepter cell counter to aspirate a sample of this dilution into the Scepter sensor.

    The Scepter cell counter’s screen displays:

    • Cell concentration
    • Average cell size
    • Average cell volume
    • Histogram of size or volume distribution

    This system is intended for research use only and has been tested with cell types representative of those in use today. Visit www.millipore.com/scepter for sample histograms, performance data, a video demonstration, and testimonials.

    The Scepter Histogram:

    “A New Window into your Cell Culture”

    • Monitor cell health
    • Check the quality of your culture
    • Ensure sample-to-sample consistency
    • Gate your histogram to count only the population that interests you

    How the Scepter cytometer delivers cell health information

    The Scepter cell counter counts any particle that passes through the orifice within the cell diameter range and will display a histogram of size distribution. Exclude aberrantly large or small cells or debris from the count by manual or automatic gating, to obtain information on the population of interest. Shifts in the histogram may reflect changing culture conditions, and these shifts can be monitored to assess cell culture quality and consistency.

    The Scepter Software:

    • 72 histograms can be saved to the Scepter device.
    • Upload data to your computer using the USB cable provided.
    • Perform additional data manipulations by exporting data to Microsoft Excel.

    Scepter Performance:

    • The Scepter cytometer counts cells or particles between 8 and 25 μm.
    • Samples should be diluted within the Scepter operating range (10,000-500,000 cells/mL).
    • A sample size of 100 μl is recommended.

    Over its operating range, the Scepter cytometer counts cells more accurately than other automated and manual counting methods (when compared to theoretical cell concentration).

    Over its operating range, the Scepter cytometer counts cells more accurately than other automated and manual counting methods (when compared to theoretical cell concentration).

    While other automated counters consume bench space and rely on object recognition software, manual focusing, and clumsy loading chambers, the Scepter™ cell counter provides true automation without the error that accompanies vision-based systems. Using Coulter principle-based technology for the ultimate in accuracy, the Scepter™ cell counter does all the work and delivers reliable cell counts and cell size information in less than 30 seconds. The histogram output reflects heterogeneous cell populations, and you can adjust gating parameters to focus on the cells that interest you most. This system is intended for research use only and has been tested with cell types representative of those in use today.

    Features & Benefits

    • The Scepter™ cell counter’s screen displays multiple pieces of data
    • Cell concentration for estimating culture density and readiness for testing or passaging
    • Average cell size for cell health and behavior related to cell dimensions
    • Average cell volume for cell health and behavior related to cell dimensions
    • Histogram of size or volume distribution for mixed populations of cells and overall cell population health

    Applications

    Cell Health Monitoring, Checking Quality of Cultures, Ensuring Sample-to-Sample Consistency

    Product Performance

    For more information visit: www.emdmillipore.com/scepter