Receptor Binding Assays

MultiScreenHTS plates provide the benefits of filtration in a convenient, automation-friendly, 96-well format.

Overview

Specifications

Ordering Information

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96-well MultiScreenHTS Plates with Durapore Membrane (PVDF) Clear Sorting & Filtering
Catalog Numbericon Descriptionicon Pore Size (µm)icon Pack Sizeicon
MSDVN6B50MultiScreenHTS DV Filter Plate, 0.65 µm, opaque, non-sterile0.6550 Show Pricing & Availability
MSGVN2B50MultiScreenHTS GV Filter Plate, 0.22 µm, opaque, non-sterile0.2250 Show Pricing & Availability
MSHVN4B50MultiScreenHTS HV Filter Plate, 0.45 µm, opaque, non-sterile0.4550 Show Pricing & Availability

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384-well MultiScreenHTS Plates with Durapore Membrane (PVDF) Clear Sorting & Filtering
Catalog Numbericon Descriptionicon Pore Size (µm)icon Pack Sizeicon
MZHVN0W10MultiScreenHTS HV Filter Plate, 0.45 µm, white, non-sterile0.4510 Show Pricing & Availability
MZHVN0W50MultiScreenHTS HV Filter Plate, 0.45 µm, white, non-sterile0.4550 Show Pricing & Availability

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96-well MultiScreenHTS Plates with Glass Fiber Filter Clear Sorting & Filtering
Catalog Numbericon Descriptionicon Pore Size (µm)icon Pack Sizeicon
MSFBN6B10MultiScreenHTS FB Filter Plate, 1.0/0.65 µm, opaque, non-sterile0.6510 Show Pricing & Availability
MSFBN6B50MultiScreenHTS FB Filter Plate, 1.0/0.65 µm, opaque, non-sterile0.6550 Show Pricing & Availability
MSFBNXB50MultiScreenHTS+ Hi Flow FB Filter Plate1.050 Show Pricing & Availability
MSFCN6B10MultiScreenHTS FC Filter Plate, 1.2/0.65 µm, opaque, non-sterile0.6510 Show Pricing & Availability
MSFCN6B50MultiScreenHTS FC Filter Plate, 1.2/0.65 µm, opaque, non-sterile0.6550 Show Pricing & Availability
MSFCNXB50MultiScreenHTS+ FC Filter Plate, Hi Flow, opaque, non-sterile1.250 Show Pricing & Availability

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384-well MultiScreenHTS Plates with Glass Fiber Filter Clear Sorting & Filtering
Catalog Numbericon Descriptionicon Pore Size (µm)icon Pack Sizeicon
MZFBN0W10MultiScreenHTS 384 FB Filter Plate, 1.0/0.65 µm, opaque, non-sterile0.6510 Show Pricing & Availability
MZFBN0W50MultiScreenHTS 384 FB Filter Plate, 1.0/0.65 µm, opaque, non-sterile0.6550 Show Pricing & Availability
MZFCN0W10MultiScreenHTS 384 FC Filter Plate, 1.2/0.65 µm, opaque, non-sterile0.6510 Show Pricing & Availability
MZFCN0W50MultiScreenHTS 384 FC Filter Plate, 1.2/0.65 µm, opaque, non-sterile0.6550 Show Pricing & Availability

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MultiScreen Harvest Plates Clear Sorting & Filtering
Catalog Numbericon Descriptionicon Pore Size (µm)icon Pack Sizeicon
MAHFB1H60MultiScreen Harvest APFB Filter Plate, opaque, 100 µL well1.060 Show Pricing & Availability
MAHFC1H60MultiScreen Harvest APFC Filter Plate, opaque, 100 µL well1.260 Show Pricing & Availability

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Accessories Clear Sorting & Filtering
Catalog Numbericon Descriptionicon Pack Sizeicon
MATAH0P00MultiScreen Sealing Tape, opaque, non-sterile100 Show Pricing & Availability
MATAHCL00MultiScreen Sealing Tape, clear, non-sterile100 Show Pricing & Availability
MAVM0960RMultiScreen™ Vacuum Manifold 96-well1 Show Pricing & Availability
MSTPCWH50Packard Top Count Adapter for MultiScreenHTS 96-well plate50 Show Pricing & Availability
MSVMHTS00MultiScreenHTS Vacuum Manifold1 Show Pricing & Availability

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    Documentation

    Technical Info

    Title
    MultiScreen HTS 384-well Glass Fiber Filter Plates
    Optimizing Radioisotope Detection Using MultiScreen Plates with Wallac Microbeta Liquid Scintillation Counters

    Data Sheet

    Title
    MultiScreen HTS Glass Fiber Filter Plates
    MultiScreen HTS Vacuum Manifold
    MultiScreen Harvest Plates

    References | 33 Available | See All References

    Reference overviewApplication
    Receptor binding assay for nitric oxide- and heme-independent activators of soluble guanylate cyclase.
    Peter Schmidt, Matthias Schramm, Henning Schroder and Johannes-Peter Stasch
    Analytical Biochemistry 314 (1): 162-165 2003

    High-throughput receptor-binding methods for somatostatin receptor 2
    Birin ET, Rohrer SP,Anal. Biochem. 2002 Aug 1; 307 (1):159-66
    Anal. Biochem. 2002 Aug 1; 307 (1):159-66 2002

    Development of 5-hydroxytryptamine(2A) receptor binding assay for high throughput screening using 96-well microfilter plates
    Harms A, Gundisch D, Muller CE, Kovar KA
    J Biomol Screen. 2000 Aug;5(4):269-78 2000

    Quantitative and functional characterization of muscarinic receptor subtypes in insulin-secreting cell lines and rat pancreatic islets
    Iismaa, Tiina P. et al.
    Diabetes, 49, 3, 392, 2000 2000

    Cell Harvesting, Receptor/Ligand Binding Assays
    An immunoassay for assessment of receptor tyrosine kinase autophosphorylation
    Grace R. Nakayama, Zahra Parandoosh
    Journal of immunological methods 225 (1999), 67-74 1999

    Quantitative cell membrane-based radioligand binding assays for parathyroid hormone receptors.
    Sam R. J. Hoare and Ted B. Usdin
    Journal of Pharmacological and Toxicological Methods 41 (2-3): 83-90 1999

    Islet cell membrane antigens activate diabetogenic (CD4.sup. +) T-cells in BB/Wor rat
    Ellerman, Karen E. et al.
    Diabetes, 48, 5, 975, 1999 1999

    Cell Harvesting, Receptor/Ligand Binding Assays
    Synthesis and Structure-Activity Relationship of a New Series of Potent AT1 Selective Angiotensin II Receptor Antagonists: 5-(Biphenyl-4-ylmethyl) pyrazoles
    Carmen, A., Gomez, L.A., Cavalcanti, F.L., deArriba, A.F., Garcia-Rafanell, J., and Forn, J.
    J. Med. Chemical 40: 547-558 1997

    Cell Harvesting, Receptor/Ligand Binding Assays
    Mutagenesis of Acidic Residues in the Oxygenase Diomain of Inducible Nitric-Oxide Synthase Identifies a Glutamate Involved in Arginine Binding
    Gachhui, R., Ghosh, D.K., Wu, C., Parkinson, J., Crane, B.R., Stuehr, D.J.
    BioChemistry. 36:17. 5097 - 5103 1997

    Cell Harvesting, Receptor/Ligand Binding Assays
    Binding sites of nitric oxide synthases
    Lui, Q., Gross, S. S.; Methods in Enzymology, 268: 3-11-324, 1996
    Methods in Enzymology, 268: 3-11-324, 1996 1996

    Cell Harvesting, Receptor/Ligand Binding Assays

    User Guides

    Title
    MultiScreen Harvest Plates
    MultiScreen®HTS and MultiScreen®HTS+ Hi Flow Assay Systems

    C7747MultiScreenHTS plates provide the benefits of filtration in a convenient, automation-friendly, 96-well format.

    Receptor-binding assays are a critical component in lead indentification and later lead characterization processes. They are used to characterize most known drug targets and typically use
    filter-based separations technology to obtain necessary “bound vs. free” fractions for assay validation. For sensitivity and specificity, radiolabeled known drugs are used in competitive binding assays. The assay is designed as a competitive inhibition assay using the radiolabeled known drug/ligand receptor interaction to screen chemical or natural product libraries for more effective NCEs (new chemical entities).

    These quantitative binding parameter determinations indicate the minimal effective drug concentrations. MultiScreenHTS plates for quantitative binding parameters provide a more accurate and reliable alternative to homogeneous assays. They are widely used in high throughput screening campaigns and provide a reliable platform that incorporates a range of glass fiber filters to retain the receptor and “bound” ligand fraction. Operation by vacuum manifold allows for more convenient characterizations since the bound fractions are easily collected from the top of the plate.

    The improved plate design of MultiScreenHTS automation-compatible
    filter plates facilitates use with gripper arms and is compatible with microplate scintillation counters.

    MultiScreenHTS+ Hi Flow filter plates: improve washing efficiency and reduce data variation.

    MultiScreen Harvest plates are also available to withstand the numerous washes and batch pretreatment (PEI) required in cell harvest protocols.

    Highly sensitive and specific radiometric assays

    • Compatible with liquid scintillation cocktail
    • Improved automation compatibility with new MultiScreenHTS filter plates

    Protocol

    1. Add cells, membrane, or tissue to MultiScreen Plate
    2. Incubate with labeled ligand
    3. Collect receptor/ligand complex on filter membrane. Wash and count. If "free vs. bound" study, collect filtrate into 96-well plate

    Performance

    Optimized Design for High Quality Results

    384-well Displacement Binding of Human Muscarinic M1 Receptor

    96-well Displacement Binding of Human Muscarinic M1 Receptor

    Radioligand binding displacement binding assays were performed with a constant radiolabeled scopolamine concentration (0.6 nM) and serial dilutions of unlabelled pirenzipine as compared to a control binding experiment without unlabelled pirenzipine (% of Control). Left: Displacement binding done with 4.38 µg receptor preparation in 100 µL using MutliScreen<sub>HTS</sub> 384-well filter plate. Results presented are from three separate experiments each performed in triplicate wells. Right: Displacement binding done with 8.75 µg receptor preparation in 200 µL using MutliScreen 96-well filter plate. Relative affinity values (IC50) were determined by fitting displacement binding inhibition values by non-linear regression using Prism™ data software. All data points are the average of triplicate experiments. Filter plates were dried prior to the addition of Supermix™ (10 µL in 384-well plates and 50 µL in 96-well plates). Counting was done in a MicroBeta® Trilux. NOTE: All counting is done with underdrain on for both 96- and 384-well plates.

    Radioligand binding displacement binding assays were performed with a constant radiolabeled scopolamine concentration (0.6 nM) and serial dilutions of unlabelled pirenzipine as compared to a control binding experiment without unlabelled pirenzipine (% of Control). Left: Displacement binding done with 4.38 µg receptor preparation in 100 µL using MutliScreenHTS 384-well filter plate. Results presented are from three separate experiments each performed in triplicate wells. Right: Displacement binding done with 8.75 µg receptor preparation in 200 µL using MutliScreen 96-well filter plate. Relative affinity values (IC50) were determined by fitting displacement binding inhibition values by non-linear regression using Prism™ data software. All data points are the average of triplicate experiments. Filter plates were dried prior to the addition of Supermix™ (10 µL in 384-well plates and 50 µL in 96-well plates). Counting was done in a MicroBeta® Trilux. NOTE: All counting is done with underdrain on for both 96- and 384-well plates.