Amicon Ultra-15 Centrifugal Filter Units

Provide both ultra-fast spin times and ultra-high recoveries

Amicon Ultra filter units process 15 mL samples as fast as 10 15 minutes

Overview

Specifications

Ordering Information

Amicon Ultra-15 Centrifugal Filter Units Clear Sorting & Filtering
Catalog Numbericon Descriptionicon NMWL (KDa)icon Pack Sizeicon
UFC900308Amicon Ultra-15 Centrifugal Filter Unit with Ultracel-3 membrane38 Show Pricing & Availability
UFC900324Amicon Ultra-15 Centrifugal Filter Unit with Ultracel-3 membrane324 Show Pricing & Availability
UFC900396Amicon Ultra-15 Centrifugal Filter Unit with Ultracel-3 membrane396 Show Pricing & Availability
UFC901008Amicon Ultra-15 Centrifugal Filter Unit with Ultracel-10 membrane108 Show Pricing & Availability
UFC901024Amicon Ultra-15 Centrifugal Filter Unit with Ultracel-10 membrane1024 Show Pricing & Availability
UFC901096Amicon Ultra-15 Centrifugal Filter Unit with Ultracel-10 membrane1096 Show Pricing & Availability
UFC903008Amicon Ultra-15 Centrifugal Filter Unit with Ultracel-30 membrane308 Show Pricing & Availability
UFC903024Amicon Ultra-15 Centrifugal Filter Unit with Ultracel-30 membrane3024 Show Pricing & Availability
UFC903096Amicon Ultra-15 Centrifugal Filter Unit with Ultracel-30 membrane3096 Show Pricing & Availability
UFC905008Amicon Ultra-15 Centrifugal Filter Unit with Ultracel-50 membrane508 Show Pricing & Availability
UFC905024Amicon Ultra-15 Centrifugal Filter Unit with Ultracel-50 membrane5024 Show Pricing & Availability
UFC905096Amicon Ultra-15 Centrifugal Filter Unit with Ultracel-50 membrane5096 Show Pricing & Availability
UFC910008Amicon Ultra-15 Centrifugal Filter Unit with Ultracel-100 membrane1008 Show Pricing & Availability
UFC910024Amicon Ultra-15 Centrifugal Filter Unit with Ultracel-100 membrane10024 Show Pricing & Availability
UFC910096Amicon Ultra-15 Centrifugal Filter Unit with Ultracel-100 membrane10096 Show Pricing & Availability

Amicon Ultra-15 Filter Units are assembled with centrifuge tubes and caps

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    Documentation

    Brochure

    Title
    Fast and Easy Sample Dialysis
    Protein purification and preparation.

    References

    Reference overviewApplication
    Simultaneous reduction and alkylation of protein disulfides in a centrifugal ultrafiltration device prior to two-dimensional gel electrophoresis.
    Smejkal GB, Li C, Robinson MH, Lazarev AV, Lawrence NP, Chernokalskaya E.
    J Proteome Res. 2006 Apr;5(4):983-7. 2006

    Protein Concentration
    Ultrafiltration for proteomic sample preparation
    E. Chernokalskaya, S. Gutierrez, A.M. Pit, J. T. Leonard
    Electrophoresis, 25: 2461-2468 2004

    Sample Prep

    FAQ

    QuestionAnswer
    What pipette or pipette tips do you recommend to recover the retentate from the Amicon Ultra concentrators?We do not have any recommendation for pipette or pipette tip in the insert-booklet of Amicon Ultra. Certain pipettes, like Eppendorf 100-1000µl, are very wide and the tip can not reach the bottom of the device. So a smaller pipette is needed. The pipette we use for all the studies is a Gilson pipetman with regular pipette tips 1-200µl from Fisher cat# 21-197-2J.
    Amicon Ultra units are offered in what volumes?Amicon Ultra units are available in 0.5 mL, 4mL & 15ml volumes.
    What is the recommended rotor for Amicon Ultra units?Any rotor capable of accommodating 22.7 cm in diameter and 124 mm length centrifuge tubes for the Amicon Ultra-4 and 35 mm in diameter and 121 mm in length for the Amicon Ultra-15 can be used for Amicon Ultra units.
    How should I select the appropriate Molecular Weight Cutoff (MWCO) for Amicon Ultra?Select a Molecular Weight Cutoff that is 2x smaller that the Molecular Weight (MW) of the solute of interest.
    Which membranes are available in Amicon Ultra units?Amicon Ultra units are only available with Ultracel regenerated cellulose material. This high recovery/low binding membrane is offered in 3,000, 10,000, 30,000, 50,000 & 100,000 MWCO.
    Can the Amicon Ultra be spun to dryness?No, the Amicon Ultra has a built in deadstop.
    Can the Amicon Ultra be used with nucleic acids?Yes, Amicon Ultra units have been evaluated for use with Genomic and PCR fragments. The 30 kDa MWCO provides the best balance between recovery and spin time. Protocols related to Genomic and PCR fragments can be found in the user guide.
    What is the maximum sample volume for Amicon Ultra?
    Amicon Ultra-4Amicon Ultra-15
    Swinging Bucket4ml 15ml
    Fixed Angle4ml(35 & 45 degree rotor angle)12ml(35 degree rotor angle)
    3.5ml(23 degree rotor angle)
    What is the maximum centrifugal force I can spin Amicon Ultra-4 & Amicon Ultra-15?
    4ml15ml
    Swinging Bucket4000 x g4000 x g
    3500 x g for concentrated/viscous solutions
    Fixed Angle(23, 35 &45 degrees)7500 x g5000 x g
    What is the chemical compatibility of Amicon Ultra?Amicon Ultra units are intended for use with biological fluids and aqueous solutions. Please refer to the user guide, catalogue, or contact Technical Support.
    Can Amicon Ultra units be sterilized with ethanol?Amicon Ultra is compatible with 70% Ethanol, but we have not done any studies to test for sterilization.
    Do you have any centrifugal filter units that are pyrogen free?We do not have any centrifugals devices that we claim are pyrogen free. Even the sterile devices could contain pyrogens.

    C7715Provide both ultra-fast spin times and ultra-high recoveries

    • High recovery Ultracel regenerated cellulose membrane in a range of molecular weight cut-offs
    • High retentate recovery of >90%
    • Vertical membrane reduces concentration polarization for ultra-fast spin times (as fast as 10–15 minutes)
    • Heat-sealed membrane minimizes downstream extractables
    • 100% integrity tested for reliable performance
    • Convenient sample monitoring with translucent housing and volume gradations
    • Direct pipettor sample access eliminates processing step to recover concentrate
    • High concentration factors of 80–100X

    Applications

    • Concentration of biological samples containing antigens, antibodies, enzymes, nucleic acids, or microorganisms
    • Purification of macromolecular components found in tissue culture extracts or cell lysates and protein removal prior to HPLS
    • Concentration of dilute or pre-purified proteins from column eluents
    • Desalting, buffer exchange and protein dialysis
    • Size exclusion and protein fractionation

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    Typical Spin Times to Filtrate Volume

    Spin conditions: 4000 xg swinging bucket rotor at 25°C, 15 mL sample. <br />3K, 10K: Cytochrome c (0.25 mg/mL); 30K, 50K: BSA (1 mg/mL); 100K: IgG (1 mg/mL).

    Spin conditions: 4000 xg swinging bucket rotor at 25°C, 15 mL sample.
    3K, 10K: Cytochrome c (0.25 mg/mL); 30K, 50K: BSA (1 mg/mL); 100K: IgG (1 mg/mL).

    Typical Protein Recovery

    Membrane
    NMWL, kDa
    Protein Solute Protein Retentate
    Volume, mL
    Protein Retentate
    Recovery, %
    3 Cytochrome c, 12.4 kDa
    (0.25 mg/mL)
    0.294 97.5
    10 Cytochrome c, 12.4 kDa
    (0.25 mg/mL)
    0.24 93.03
    30 BSA, 67 kDa
    (1 mg/mL)
    0.30 97.94
    50 BSA, 67 kDa
    (1 mg/mL)
    0.17 93.32
    100 IgG, 156 kDa
    (1 mg/mL)
    0.28 89.03
    15 mL starting volume in swinging bucket rotor at 4000 x g at 25 °C. Spin times: 3K (40 min); 10K and 100K (20 min); 30K (10 min); 50K (15 min).
    Volumes
    Maximum Initial Sample Volume, mL* 15
    Typical Final Concentrate Volume, µL 200
    Centrifugal Force Swinging bucket rotor: 4,000 x g; Fixed angle rotor (35°): 5,000 x g
    Materials
    Membrane Ultracel regenerated cellulose
    Device Housing: Styrene/butadiene
    Tube: Polypropylene
    Cap and liner: Polyethylene
    Dimensions
    Active Membrane Area, cm2 7.6
    Diameter, mm 29.7
    Filtrate tube or vial (centrifuge tube)
    Length, mm 122
    Filter unit
    Diameter, mm 29.6
    Length, mm 72.0
    *Except for 35° fixed angle rotor: 12 mL

    Amicon Ultra-15 Centrifugal Filter Unit with Ultracel-10 membrane is CE marked.