Amicon Ultra-15 Centrifugal Filter Units

Provide both ultra-fast spin times and ultra-high recoveries

Overview

Specifications

Ordering Information

Amicon Ultra-15 Centrifugal Filter Units Clear Sorting & Filtering
Catalog Numbericon Descriptionicon NMWLicon Pack Sizeicon
UFC900308Amicon Ultra-15 Centrifugal Filter Unit with Ultracel-3 membrane 3 KDa 8 Show Pricing & Availability
UFC900324Amicon Ultra-15 Centrifugal Filter Unit with Ultracel-3 membrane 3 KDa 24 Show Pricing & Availability
UFC900396Amicon Ultra-15 Centrifugal Filter Unit with Ultracel-3 membrane 3 KDa 96 Show Pricing & Availability
UFC901008Amicon Ultra-15 Centrifugal Filter Unit with Ultracel-10 membrane 10 KDa 8 Show Pricing & Availability
UFC901024Amicon Ultra-15 Centrifugal Filter Unit with Ultracel-10 membrane 10 KDa 24 Show Pricing & Availability
UFC901096Amicon Ultra-15 Centrifugal Filter Unit with Ultracel-10 membrane 10 KDa 96 Show Pricing & Availability
UFC903008Amicon Ultra-15 Centrifugal Filter Unit with Ultracel-30 membrane 30 KDa 8 Show Pricing & Availability
UFC903024Amicon Ultra-15 Centrifugal Filter Unit with Ultracel-30 membrane 30 KDa 24 Show Pricing & Availability
UFC903096Amicon Ultra-15 Centrifugal Filter Unit with Ultracel-30 membrane 30 KDa 96 Show Pricing & Availability
UFC905008Amicon Ultra-15 Centrifugal Filter Unit with Ultracel-50 membrane 50 KDa 8 Show Pricing & Availability
UFC905024Amicon Ultra-15 Centrifugal Filter Unit with Ultracel-50 membrane 50 KDa 24 Show Pricing & Availability
UFC905096Amicon Ultra-15 Centrifugal Filter Unit with Ultracel-50 membrane 50 KDa 96 Show Pricing & Availability
UFC910008Amicon Ultra-15 Centrifugal Filter Unit with Ultracel-100 membrane 100 KDa 8 Show Pricing & Availability
UFC910024Amicon Ultra-15 Centrifugal Filter Unit with Ultracel-100 membrane 100 KDa 24 Show Pricing & Availability
UFC910096Amicon Ultra-15 Centrifugal Filter Unit with Ultracel-100 membrane 100 KDa 96 Show Pricing & Availability

Amicon Ultra-15 Filter Units are assembled with centrifuge tubes and caps

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    Documentation

    Brochure

    Title
    Protein Purification and Preparation

    Posters

    Title
    A centrifugal ultrafiltration-based method for enrichment of microvesicles

    References

    Reference overviewApplication
    Simultaneous reduction and alkylation of protein disulfides in a centrifugal ultrafiltration device prior to two-dimensional gel electrophoresis.
    Smejkal GB, Li C, Robinson MH, Lazarev AV, Lawrence NP, Chernokalskaya E.
    J Proteome Res. 2006 Apr;5(4):983-7.  2006

    Protein Concentration
    Ultrafiltration for proteomic sample preparation
    E. Chernokalskaya, S. Gutierrez, A.M. Pit, J. T. Leonard
    Electrophoresis, 25: 2461-2468  2004

    Sample Prep

    FAQ

    QuestionAnswer
    What pipette or pipette tips do you recommend to recover the retentate from the Amicon Ultra concentrators? We do not have any recommendation for pipette or pipette tip in the insert-booklet of Amicon Ultra. Certain pipettes, like Eppendorf 100-1000µl, are very wide and the tip can not reach the bottom of the device. So a smaller pipette is needed. The pipette we use for all the studies is a Gilson pipetman with regular pipette tips 1-200µl from Fisher cat# 21-197-2J.
    Amicon Ultra units are offered in what volumes? Amicon Ultra units are available in 0.5 mL, 4mL & 15ml volumes.
    What is the recommended rotor for Amicon Ultra units? Any rotor capable of accommodating 22.7 cm in diameter and 124 mm length centrifuge tubes for the Amicon Ultra-4 and 35 mm in diameter and 121 mm in length for the Amicon Ultra-15 can be used for Amicon Ultra units.
    How should I select the appropriate Molecular Weight Cutoff (MWCO) for Amicon Ultra? Select a Molecular Weight Cutoff that is 2x smaller that the Molecular Weight (MW) of the solute of interest.
    Which membranes are available in Amicon Ultra units? Amicon Ultra units are only available with Ultracel regenerated cellulose material. This high recovery/low binding membrane is offered in 3,000, 10,000, 30,000, 50,000 & 100,000 MWCO.
    Can the Amicon Ultra be spun to dryness? No, the Amicon Ultra has a built in deadstop.
    Can the Amicon Ultra be used with nucleic acids? Yes, Amicon Ultra units have been evaluated for use with Genomic and PCR fragments. The 30 kDa MWCO provides the best balance between recovery and spin time. Protocols related to Genomic and PCR fragments can be found in the user guide.
    What is the maximum sample volume for Amicon Ultra?
    Amicon Ultra-4Amicon Ultra-15
    Swinging Bucket4ml 15ml
    Fixed Angle4ml(35 & 45 degree rotor angle)12ml(35 degree rotor angle)
    3.5ml(23 degree rotor angle)
    What is the maximum centrifugal force I can spin Amicon Ultra-4 & Amicon Ultra-15?
    4ml15ml
    Swinging Bucket4000 x g4000 x g
    3500 x g for concentrated/viscous solutions
    Fixed Angle(23, 35 &45 degrees)7500 x g5000 x g
    What is the chemical compatibility of Amicon Ultra? Amicon Ultra units are intended for use with biological fluids and aqueous solutions. Please refer to the user guide, catalogue, or contact Technical Support.

    User Guides

    Title
    Amicon® Ultra-15 3K, 30K, 50K, and 100K Centrifugal Filter User Guide