SNAP i.d.® 2.0 Protein Detection System

Rapid system takes protein detection to new dimensions

Overview

Specifications

Ordering Information

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SNAP i.d. 2.0 Hardware Clear Sorting & Filtering
Catalog Numbericon Descriptionicon Pack Sizeicon
SNAP2MIDISNAP i.d. 2.0 Protein Detection System-Midi (8.5 x 13.5 cm)1/pk Show Pricing & Availability
SNAP2MINISNAP i.d. 2.0 Protein Detection System-Mini (7.5 x 8.4 cm)1/pk Show Pricing & Availability
SNAP2MMSNAP i.d. 2.0 Protein Detection System-Mini and Midi (7.5 x 8.4 cm and 8.5 x 13.5 cm)1/pk Show Pricing & Availability

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SNAP i.d. 2.0 Consumables & Hardware Clear Sorting & Filtering
Catalog Numbericon Descriptionicon Pack Sizeicon
SNAP2BHMD0100SNAP i.d.® 2.0 Midi Blot Holders (8.5 x 13.5 cm)100/pk Show Pricing & Availability
SNAP2BHMN0100SNAP i.d.® 2.0 Mini Blot Holders (7.5 x 8.4 cm)100/pk Show Pricing & Availability
SNAP2FRMD01SNAP i.d.® 2.0 Midi Blot Holding Frame (single pack)1/pk Show Pricing & Availability
SNAP2FRMD02SNAP i.d.® 2.0 Midi Blot Holding Frames (double pack)2/pk Show Pricing & Availability
SNAP2FRMN01SNAP i.d.® 2.0 Mini Blot Holding Frame (single pack)1/pk Show Pricing & Availability
SNAP2FRMN02SNAP i.d.® 2.0 Mini Blot Holding Frames (double pack)2/pk Show Pricing & Availability
SNAP2RLSNAP i.d.® 2.0 Blot Roller1/pk Show Pricing & Availability
SNAPABTRSNAP i.d.® 2.0 Antibody Collection Tray20/pk Show Pricing & Availability

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SNAP i.d. Consumables & Hardware Clear Sorting & Filtering
Catalog Numbericon Descriptionicon Pack Sizeicon
WBAVDABTRSNAP i.d. Antibody Collection Tray20 Show Pricing & Availability
WBAVDBH01SNAP i.d. Single Well Blot Holder30 Show Pricing & Availability
WBAVDBH011SNAP i.d. Single Well Blot Holder60 Show Pricing & Availability
WBAVDBH02SNAP i.d. Double Well Blot Holder30 Show Pricing & Availability
WBAVDBH022SNAP i.d. Double Well Blot Holder60 Show Pricing & Availability
WBAVDBH03SNAP i.d. Triple Well Blot Holder20 Show Pricing & Availability
WBAVDBH033SNAP i.d. Triple Well Blot Holder40 Show Pricing & Availability

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SNAP i.d. Accessory Hardware Clear Sorting & Filtering
Catalog Numbericon Descriptionicon Pack Sizeicon
WP6110060Chemical Duty Pump, 100 V/50–60 Hz1 Show Pricing & Availability
WP6111560Chemical Duty Pump, 115 V/60 Hz1 Show Pricing & Availability
WP6122050Chemical Duty Pump, 220 V/50 Hz1 Show Pricing & Availability
XX1004705Vacuum filtering flask, 1 L1 Show Pricing & Availability
XX1004708No. 8 perforated stopper, silicone5 Show Pricing & Availability
XX6200006PFilter forceps, blunt end, stainless steel3 Show Pricing & Availability

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    Documentation

    Brochure

    Title
    Bundle-up your Western blots for unbeatable savings!
    Western Blotting Tools

    Technical Info

    Title
    Antibody recovery and reuse in the SNAP i.d.® 2.0 immunodetection system
    Fluorescent Immunodetection Using the SNAP i.d. Protein Detection System
    Key Steps for Successful Immunodetection Using the SNAP i.d.™ Protein Detection System
    Triple Well Blot Holder as a Tool for 30-Minute Western Blotting Optimization with the SNAP i.d. Protein Detection System

    References | 18 Available | See All References

    Reference overviewApplication
    Heterologous expression of human membrane receptors in the yeast Saccharomyces cerevisiae
    Joubert O, Nehmé R, Bidet M, Mus-Veteau I.
    Methods Mol Biol. 601:87-103 2010

    Western Blotting
    Rab13 regulates neurite outgrowth in PC12 cells through its effector protein
    Sakane A, Honda K, Sasaki T.
    JRAB/MICAL-L2. Mol Cell Biol.; 30(4):1077-87 2010

    Translational regulation of gene expression by an anaerobically induced small non-coding RNA in Escherichia coli
    Boysen A, Moller-Jensen J, Kallipolitis BH, Valentin-Hansen P, Overgaard M.
    J Biol Chem. 2010

    Western Blotting
    Suppression of Adipocyte differentiation by aldo-keto reductase 1B3 acting as prostaglandin F2α synthase
    Fujimori K, Ueno T, Nagata N, Kashiwagi K, Aritake K, Amano F, Urade Y.
    J Biol Chem. 2010

    Western Blotting
    Impact of the DNA methyltransferases expression on the methylation status of apoptosis-associated genes in glioblastoma multiforme
    Hervouet E, Vallette FM, Cartron P-F.
    Cell Death and Disease 1:1-9 2010

    Western Blotting
    Overexpression of protein disulfide isomerases enhances secretion of recombinant human transferrin in Schizosaccharomyces pombe
    Mukaiyama H, Tohda H, Takegawa K.
    Appl Microbiol Biotechnol 2009

    Western Blotting
    Short-hairpin RNAs delivered by lentiviral vector transduction trigger RIG-I-mediated IFN activation
    Kenworthy R, Lambert D, Yang F, Wang N, Chen Z, Zhu H, Zhu F, Liu C, Li K, Tang H.
    Nucleic Acids Res. 37(19):6587-99. 2009

    Western Blotting
    Profile for Amyloid-beta and Tau Expression in Primary Cortical Cultures from 3xTg-AD Mice
    Vale C, Alonso E, Rubiolo JA, Vieytes MR, Laferla FM, Giménez-Llort L, Botana LM.
    Cell Mol Neurobiol. 2009

    Western Blotting
    Differential roles of the glycogen-binding domains of beta subunits in regulation of the Snf1 kinase complex.
    Mangat S, Chandrashekarappa D, McCartney RR, Elbing K, Schmidt MC.
    Eukaryot Cell. 2009

    Western Blotting
    Dextran sodium sulfate enhances secretion of recombinant human transferrin in Schizosaccharomyces pombe
    Mukaiyama H, Giga-Hama Y, Tohda H, Takegawa K.
    Appl Microbiol Biotechnol; 85(1):155-64 2009

    Western Blotting

    User Guides

    Title
    Antibody Collection Tray - Instructions for Use with the SNAP i.d. Protein Detection System
    Blot Holder: Instructions for use with SNAP i.d. Protein Detection System User Guide
    Quick Start Guide - SNAP i.d.® 2.0 IHC (Immunohistochemistry) System
    SNAP i.d. Protein Detection System
    SNAP i.d. Protein Detection System - Overview of Procedure
    SNAP i.d.® 2.0 Antibody Collection Tray
    SNAP i.d.® 2.0 Blot Holder and Frame
    SNAP i.d.® 2.0 Protein Detection System User Guide
    SNAP i.d.® 2.0 Protein Detection System for Immunohistochemistry User Guide
    SNAP i.d.® 2.0 Quick Start Guide

    C73105Rapid system takes protein detection to new dimensions

    Developed to meet the needs of our Western blotting customers, the SNAP i.d.® 2.0 system produces blots of a very high quality every time – in record time! Unique vacuum-driven technology and a built-in flow distributor actively drive reagents through the membrane, ensuring even distribution. Two blot holder sizes accommodates mini Mini (7.5 x 8.4 cm) or midi Midi (8.5 x 13.5 cm) (W x L) ) size gels and two blot holders can be run in parallel. Thus, you can quickly optimize conditions and greatly increase your protein detection throughput.

    Typically, researchers lack the time to optimize their blotting protocols. By shortening the time required for blocking, washing and antibody incubations to 30 minutes, the SNAP i.d.® 2.0 system allows you to optimize your immunodetection conditions for the highest quality results.

    The ordering tables on the next tab show how you can easily obtain the SNAP i.d.® 2.0 system and its components. We look forward to hearing about your research results once you have used your new tool for Western blotting!

    SNAP i.d.® 2.0 Hardware and Accessories

    SNAP i.d.® 2.0 Mini Blot Holder

    SNAP i.d.® 2.0 Mini Blot Holder

    SNAP i.d.® 2.0 Midi Blot Holder

    SNAP i.d.® 2.0 Midi Blot Holder

    SNAP i.d.® 2.0 Antibody Collection Tray

    SNAP i.d.® 2.0 Antibody Collection Tray

    SNAP i.d.® 2.0 Blot Roller

    SNAP i.d.® 2.0 Blot Roller

    Your SNAP i.d.® 2.0 system includes everything you need to get started, including blot holders, two independent blot holder frames, antibody collection tray and blot roller. In addition, we provide you with an integrated vacuum regulator to ensure consistent vacuum pressure without the need for an external regulator.

    Other useful items include: Chemical duty pumps (115 V/60 Hz) or (220 V/50 Hz), a one-liter Vacuum filtering flask, Silicone No. 8 perforated stoppers (5 to a pack) and stainless steel forceps.

    Unlike conventional Western blotting, where diffusion is the primary means of reagent transport, the SNAP i.d.® 2.0 system uses a vacuum to actively drive reagents through the membrane, enabling thorough washing and reducing the blocking, antibody incubation and wash steps to 30 minutes total. The SNAP i.d.® 2.0 system adds exciting new capabilities for Western blotting and requires no additional reagent consumption (e.g., antigen, antibody or detection reagents). The system’s unique design enables the use of small volumes for antibody incubations with either polyvinylidene difluoride (PVDF) or nitrocellulose blotting membranes.

    Features & Benefits

    • Superior: Increased antibody-antigen binding, enhanced washes, and antibody recollection
    • Flexible: Two gel sizes, mini (7.5 x 8.4 cm) and midi (8.7 x 13.5 cm)
    • Fast: 30-minute immunodetection

    Applications

    Western Blotting using Nitrocellulose or PVDF Membrane; Compatible with Radioactive, Chromogenic, Chemiluminescent, Fluorescent, and Chemifluorescent Detection

    Product Performance

    For more information visit: www.emdmillipore.com/cellculture