Stirred Cells

Pressure-based sample concentration

Overview

Specifications

Ordering Information

Stirred Cells Clear Sorting & Filtering
Catalog Numbericon Descriptionicon Pack Sizeicon
5121Stirred Cell Model 8010, 10 mL1 Show Pricing & Availability
5122Stirred Cell Model 8050, 50 mL1 Show Pricing & Availability
5123Stirred Cell Model 8200, 200 mL1 Show Pricing & Availability
5124Stirred Cell Model 8400, 400 mL1 Show Pricing & Availability
5125Stirred Cell Model 8003, 3 mL1 Show Pricing & Availability

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Replacement Part Kits Clear Sorting & Filtering
Catalog Numbericon Descriptionicon Pack Sizeicon
8000SCPKITSmall Replacement Parts for 8000 Series Stirred Cells1 kit Show Pricing & Availability
8003SCPKITLarge Replacement Parts for 8003 Series Stirred Cells1 kit Show Pricing & Availability
8010SCPKITLarge Replacement Parts for 8010 Series Stirred Cells1 kit Show Pricing & Availability
8050SCPKITLarge Replacement Parts for 8050 Series Stirred Cells1 kit Show Pricing & Availability
8200SCPKITLarge Replacement Parts for 8200 Series Stirred Cells1 kit Show Pricing & Availability
8400SCPKITLarge Replacement Parts for 8400 Series Stirred Cells1 kit Show Pricing & Availability

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    Documentation

    Data Sheet

    Title
    Series 8000 Stirred Cells and Ultrafiltration Membranes

    References | 35 Available | See All References

    Reference overviewApplication
    Continuous production of oligosaccharides from whey using a membrane reactor
    Foda MI; Lopez Leiva M (reprint)
    Process Biochemistry 2000, V35, N6, P581-587 SN- 0032-9592 2000

    Electrokinetic characterisation of ultrafiltration membranes by streaming potential, electroviscous effect, and salt retention
    Huisman IH (REPRINT) ; Pradanos P; Hernandez A
    Journal of membrane science 2000, V178, N1-2, p55-64 2000 2000

    Integrated process for the removal of emulsified oils from effluents in the steel industry
    Benito JM; Rios G; Gutierrez B; Pazos C; Coca J (REPRINT)
    Separation Science and Technology 1999, V34, N15, P3031-3043 SN- 0149-6395 1999

    Industrial QC
    Distribution of trace elements associated with dissolved compounds (<0.45 .mu.m-1 nm) in freshwater using coupled (frontal cascade) ultrafiltration and chromatographic separations
    Pham, M. K. Garnier, J.-M.
    Environmental Science and Technology, v32, pg. 440-449, 15 Feb. 1998 1998

    Performance Characteristics of Waste Oil Emulsion Treatment by Ultrafiltration
    Lin, S. H.; Lan, W. J. Yuan Ze University, Taoyuan, Taiwan
    J Environ Sci Health-Toxic Hazard Subst Environ Eng 1998,SO- vA33, n3, p385(20) 1998

    General approach for the development of high-performance liquid chromatography methods for biosurfactant analysis and purification
    Lin, S.-C. Chen, Y.-C. Lin, Y.-M.
    J. Chromatogr., A ; Journal of Chromatography, A v. 825 pg 149-159 6 Nov 1998 1998

    Filtration 15 nm: Aspects Techniques
    Chtourou S,Sang thrombose vaisseaux, 21-27, 1998
    Sang thrombose vaisseaux, 21-27, 1998 1998

    Acrylamide production in an ultrafiltration-membrane bioreactor using cells of Brevibacterium imperialis CBS 489-74
    Cantarella M (reprint) ; Spera A; Cantarella L; Alfani F ; Journal of membrane science, v147, N2, p279-290 1998
    Journal of membrane science, v147, N2, p279-290 1998 1998

    Enzyme Assay
    In vitro and in vivo grafting of xeno pig fetal liver fragments using ultrafiltration membrane
    Kanai N.; Morita N.; Munkhbat B.; Gansuvd B.; Hagihara M.; Nagamachi Y.;Tsuji K.
    Cell Transplantation SO- 7/4 (417-420) 1998 1998

    Cell Culture
    Metabolic screening using on-line ultrafiltration mass spectrometry
    Van Breemen R.B.; Nikolic D.; Bolton J.L.
    Drug Metabolism and Disposition , 26/2 (85-90) 1998 1998

    FAQ

    QuestionAnswer
    Are the Stirred Cells autoclavable?Yes, stirred cells can be autoclaved at 121 C for 30 minutes. It is important, however, to tighten the base the loosen 1/4 turn before autoclaving. The tubing and the tubing connector are not autoclavable.
    Are there cases where a stirred cell outperforms a crossflow type system?Yes, at protein levels below 10 ug/ml. Also, pressure has a greater effect on flux as protein concentrations decrease. Most laboratory tangential flow systems operate at low pressure (20-25 psi), due to peristaltic pump limitations. Stirred cells do not require a pump for operation. They are normally pressurized to 55 psi.
    Can I use compressed air instead of nitrogen to pressurize the cell?We don't recommended it because air may cause pH shifts (C02 adsorption) or oxidation of product.
    Can the membranes for the Stirred Cells be reused?Yes, the membranes can be reused several times given they are used, cleaned, and stored properly. The operating instructions for the Ultrafiltration Membranes (literature number 99101) includes a restoration protocol for the membranes specific to each type. Also, under the operating procedures section is the proper method for usage and storage.
    Can the Stirred Cell products be sanitized with Sodium Hypochlorite?Yes. We recommend 100 ppm (parts per million) of Sodium Hypochlorite for 30 min.
    Can the stirred cell be autoclaved with a membrane in place?Yes, but only with the YM membrane. Put a small amount of buffer in the device to keep the membrane wet, tighten the bottom then loosen 1/4 turn. Remove any tubing and fittings, they are not autoclavable.
    Do you sell parts for the Stirred Cells individually? What is the part number and price?Yes, the parts lists for the Model 8000 Stirred Cells can be found in the Stirred Ultrafiltration User's Guide P99228. The parts list for the Model 2000 High Output Stirred Cell can be found in the user's guide P99109. Parts for the older style Stirred Cells (Model 12, 52, 202 or 402) are no longer being replaced.
    Does tubing come with the stirred cell?Yes, the following tubing comes with the stirred cell: a. Four feet of 1/4" pressure tubing for pressurizing the stirred cell. b. Four inches of permeate tubing.
    How do I know the membrane in my stirred cells is in correctly?The glossier side of the membrane should be facing up.
    How do I remove the stirred cell after I've vented the pressure?Push the cap down, then slide the cell out from the retaining stand.
    How do I sterilize my stirred cell?The 8000 series can be sterilized using any one of the following: 70% Ethanol 70% Isopropanol 5% Formaldehyde <200 ppm NaOCl (sanitize) The Model 2000 can be sterilized using either of the following: 5% Formaldehyde <200 ppm NaOCL (sanitize)
    I bought some replacement bodies for my old style stirred cell. They did not fit properly. Has there been a product change?In March of 1990 the stirred cell bases were changed to a white plastic molded part from a black metal part. Bodies compatible with the black metal base are not compatible with the white plastic base. When ordering a replacement body you will also need to order a new base. Here are the catalogue numbers for the affected products:
    CAT #DescriptionBodyBase
    5125Model 8003153270521000
    5121Model 8010 153270121000
    5122Model 8050153270220987
    5123Model 8200153270321011
    5124Model 8400153270421003
    I don't have any flow in my stirred cell. What happened?a. Cell is not pressurized.
    b. Membrane upside down. Glossy side should face up.
    c. Plastic sheet not removed from membrane (XM-300 only),
    d. Solution may be too concentrated (viscous).
    e. Check that the membrane holder and filtrate port is not blocked.
    In what order do I assemble the parts to the tube fitting assembly?Assemble in the following order: First, the hexagonal nut slides on to the red pressure tubing. The metal grab ring with flange facing away from hex nut fits into hex nut. The white plastic spacer with countersink surface towards grab ring is next. Finally, the o-ring is placed onto the end of the assembly.
    What comes with the 8000 series stirred cells?Everything except the magnetic stirrer, pressure source and the membrane.
    What is the minimum volume that the stirred cells can concentrate to?Amicon stirred cells can concentrate down to the minimum volumes listed below:
    ModelCell Capacity (ml) Minimum Vol
    800330.05 ml
    801010 1 ml
    805050 2.5 ml
    82002005 ml
    840040010 ml
    20002000 50 ml
    Where can I order the filtrate tubing to the Stirred Cell (Cat. No. 4500430) when it is no longer offered at Millipore?Cat. #4500430, TUBING (TYGON R-3603, 5/32X3/32), can be purchased through Fisher Scientific (800-766-7000) or Cole Palmer (800-323-4340)
    Should I use the Ultracel regenerated cellulose membrane or the Biomax membrane in my TFF system?It depends on the nature of your process volume and the process speed required. The Ultracel membrane is a lower protein binding membrane that should be used in low protein loading applications(<20 g/m2). The Biomax membrane is a faster flowing membrane that should be used in high protein loading applications (>20 g/m2). Ultracel is less tolerant to extreme pH conditions than the Biomax, but the Ultracel is more compatible with organic solvents.
    What products and catalog numbers are required for the diafiltration set-up?The diafiltration set-up requires the following:

    1. Stirred Cell, for example catalogue number 5121 - Model 8010 (which uses 25mm membrane discs).
    2. Magnetic stirring table.
    3. Reservoir or dispensing pressure vessel. For example, catalogue number 6028 - RC800 Reservoir (800mL) or catalogue number XX6700P01 - 3.785 liter Dispensing Pressure Vessel (this will require relief valve, hose connectors, etc.)
    4. CDS10 Concentration/Dialysis Selector, catalogue number 6003.
    5. Pressure tubing to connect the system to a pressure source. XXPE00010 is 10 feet of 1/4" OD polyethylene tubing.
    6. Pressure source
    What products should I purchase to continuously diafilter?Pressurized Reservoir:
    Model 8003 - RC800 (product no. 6028),
    1/4" pressure tubing (product no. XXPE00010)
    Concentration/Dialysis Selector CDS10 (product no. 6003)
    What fitting do I need to connect the stirred cell to a regulator?Tubing adapter kit (product number 6022) provides two fittings that should fit any regulator.
    To what protein concentration can the solution be concentrated to using a stirred cell?Practical limit is 10% by weight at room temp and 55psi. Slightly higher if solution can be warmed.
    Which membranes should I use in my stirred cell?The following list indicates the appropriate disc size for the various stirred cells listed below:
    Model Membrane Diameter (mm)
    8003 25
    8010 25
    8050 43
    8200 62
    8400 76
    2000 150
    What pressures are Stirred Cells operated at?The maximum operating pressures for Models 8003, 8010, 8050, 8200 & 8400 is 75psi and Model 2000 is 100 psi. The recommended operating pressure for all Stirred Cells is 55 psi and <10psi when using a 100,000 MWCO membrane or greater.
    What concentration can I use with the Stirred Cells?All standard stirred cells operate within the following range:
    • Minimum initial concentration 0.05g%
    • Maximum Final Concentration 10g%
    What could be wrong if the stirrer assembly in the Stirred Cell won't spin?The stir bar won't spin unless the stirred cell is pressurized.

    User Guides

    Title
    Stirred Ultrafiltration Cell User Guide

    C3259Pressure-based sample concentration

    Amicon stirred cells provide high flow rates with solutions up to 10% macrosolute concentration. Capable of rapid concentration, or salt removal followed by concentration in the same unit. Complete product recoveries can generally be achieved using the diafiltration set-up as illustrated on this page.

    Gentle magnetic stirring minimizes concentration polarization and shear denaturation. All stirred cells can be autoclaved.

    Diafiltration set-up and operation with Amicon selector valve, stirred cell and accessory reservoir.<br />1 - Ultrafiltrate, 2 - Ultrafiltration equipment, 3 - Pressure supply, 4 - Selector valve, 5 - Inlet, 6 - Outlet, 7 - Feed liquid, 8 - Reservoir

    Diafiltration set-up and operation with Amicon selector valve, stirred cell and accessory reservoir.
    1 - Ultrafiltrate, 2 - Ultrafiltration equipment, 3 - Pressure supply, 4 - Selector valve, 5 - Inlet, 6 - Outlet, 7 - Feed liquid, 8 - Reservoir

    Need to concentrate your sample gently, without centrifugation? Amicon® stirred cells concentrate samples rapidly but gently, using magnetic stirring to minimize concentration polarization and shear stress-induced denaturation. Providing high flow rates with solutions up to 10% macrosolute concentration, Amicon® stirred cells also enable salt removal followed by concentration in the same unit. Complete product recoveries can generally be achieved using the diafiltration set-up. All stirred cells can be autoclaved. Amicon® stirred cells are available in five different sizes: 3 mL, 10 mL, 50 mL, 200 mL and 400 mL.

    Features & Benefits

    • Rapid concentration
    • Available in five different sizes
    • Simple, easy-to-use system
    • Autoclavable
    • Open platform is compatible with different membranes

    Applications

    Concentration, Buffer Exchange, Desalting

    For more information visit: www.emdmillipore.com/psp

    Materials
    Cap and tube
    fitting assembly
    Nylon
    Cylinder and membrane holder Polysulfone
    Stirring assembly Acetal,
    polysulfone
    O-rings Silicone rubber
    Pressure tube Polyethelene
    Filtrate tube Tygon® tubing
    Retaining stand Anodized
    aluminum or nylon
    Stirred Cell Model 8003
    Cat. No. 5125
    Model 8010
    Cat. No. 5121
    Model 8050
    Cat. No. 5122
    Model 8200
    Cat. No. 5123
    Model 8400
    Cat. No. 5124
    Max. Process Volume, mL 3 10 50 200 400
    Min. Process Volume, mL 0.075 1.0 2.5 5.0 10.0
    Membrane Diameter, mm 25 25 44.5 63.5 76
    Effective Membrane
    Area, cm2
    0.9 4.1 13.4 28.7 41.8
    Hold-up Volume, mL 0.07 0.2 0.5 1.2 1.5
    Height, cm 7.7 7.7 9.8 12.8 15.5
    Base, cm 6 x 6 6 x 6 7 x 7 9 x 9 11 x 11
    Weight, kg 0.1 0.1 0.2 0.4 0.6