|Series 8000 Stirred Cells and Ultrafiltration Membranes|
References | 35 Available | See All References
|Continuous production of oligosaccharides from whey using a membrane reactor |
Foda MI; Lopez Leiva M (reprint)
Process Biochemistry 2000, V35, N6, P581-587 SN- 0032-9592 2000
|Electrokinetic characterisation of ultrafiltration membranes by streaming potential, electroviscous effect, and salt retention |
Huisman IH (REPRINT) ; Pradanos P; Hernandez A
Journal of membrane science 2000, V178, N1-2, p55-64 2000 2000
|Integrated process for the removal of emulsified oils from effluents in the steel industry |
Benito JM; Rios G; Gutierrez B; Pazos C; Coca J (REPRINT)
Separation Science and Technology 1999, V34, N15, P3031-3043 SN- 0149-6395 1999
|Distribution of trace elements associated with dissolved compounds (<0.45 .mu.m-1 nm) in freshwater using coupled (frontal cascade) ultrafiltration and chromatographic separations |
Pham, M. K. Garnier, J.-M.
Environmental Science and Technology, v32, pg. 440-449, 15 Feb. 1998 1998
|Performance Characteristics of Waste Oil Emulsion Treatment by Ultrafiltration |
Lin, S. H.; Lan, W. J. Yuan Ze University, Taoyuan, Taiwan
J Environ Sci Health-Toxic Hazard Subst Environ Eng 1998,SO- vA33, n3, p385(20) 1998
|General approach for the development of high-performance liquid chromatography methods for biosurfactant analysis and purification |
Lin, S.-C. Chen, Y.-C. Lin, Y.-M.
J. Chromatogr., A ; Journal of Chromatography, A v. 825 pg 149-159 6 Nov 1998 1998
|Filtration 15 nm: Aspects Techniques |
Chtourou S,Sang thrombose vaisseaux, 21-27, 1998
Sang thrombose vaisseaux, 21-27, 1998 1998
|Acrylamide production in an ultrafiltration-membrane bioreactor using cells of Brevibacterium imperialis CBS 489-74 |
Cantarella M (reprint) ; Spera A; Cantarella L; Alfani F ; Journal of membrane science, v147, N2, p279-290 1998
Journal of membrane science, v147, N2, p279-290 1998 1998
|In vitro and in vivo grafting of xeno pig fetal liver fragments using ultrafiltration membrane |
Kanai N.; Morita N.; Munkhbat B.; Gansuvd B.; Hagihara M.; Nagamachi Y.;Tsuji K.
Cell Transplantation SO- 7/4 (417-420) 1998 1998
|Metabolic screening using on-line ultrafiltration mass spectrometry |
Van Breemen R.B.; Nikolic D.; Bolton J.L.
Drug Metabolism and Disposition , 26/2 (85-90) 1998 1998
|Fouling of microfiltration membranes by broth-free antifoam agents |
Liew MKH; Fane AG (reprint) ; Rogers PL
Biotechnology and Bioengineering 1997, v56, n1, p89-98 1997
|Fouling effects of yeast culture with antifoam agents on microfilters |
Liew M.K.H.; Fane A.G.; Rogers P.L.
Biotechnology and Bioengineering 1997 1997
|Retention of copper(II)-EDTA chelates from dilute aqueous solutions by a polyelectrolyte-enhanced ultrafiltration process |
Juang R.-S.; Chen M.-N.; Journal of Membrane Science SO- 119/1 (25-37) 1996
Journal of Membrane Science SO- 119/1 (25-37) 1996 1996
|Partial characterisation of human melanoma cell-derived factors that stimulate fibroblast glycosaminoglycan synthesis |
Godden JL; Edward M (REPRINT) ; Grant AW; MacKie RM
Pathobiology, v64, n5, p262-268 1996 1996
|Immunopotency of a viral particle peptide assembled on the carbohydrate moieties of self immunoglobulins |
Brumeanu, T.D.,Nature Biotechnology, vol 14, pp. 722-725
Nature Biotechnology, vol 14, pp. 722-725 1996
|Ultrafiltration of hematite sol |
Milonjic, S.K.; Separation Science and Technology, vol. 31, no. 10, pp. 1515-1521
Separation Science and Technology, vol. 31, no. 10, pp. 1515-1521 1996
|Hydraulic resistance and fouling of microfilters by Candida utilis in fermentation broth |
Liew M.K.H.; Fane A.G.; Rogers P.L.
Biotechnology and Bioengineering ,48/2 (108-117) ,1995, 1995
|Modification of an in-vitro method for estimating the bioavailability of zinc and calcium from foods |
Li-Hua Shen et al.
Zeitschrift fuer Lebensmittel-Untersuchung und -Forschung 1994 v199 no 6 pg 442-445 1994
|A comparative study of techniques used for porous membrane characterization: Pore characterization |
Kim K.J.; Fane A.G.; Ben Aim R.; Liu M.G.; Jonsson G.; Tessaro I.C.; Broek A.P.; Bargeman D. ; Journal of Membrane Science (35-46)1994
Journal of Membrane Science (35-46)1994 1994
|A pre-formed pyrogenic factor released by lipopolysaccharide stimulated macrophages |
Zampronio, A. R.,Mediators of Inflammation, vol.3, no. 5, pp. 365-373
Mediators of Inflammation, vol.3, no. 5, pp. 365-373 1994
|Comparative study of techniques used for porous membrane characterization: Pore characterization |
Kim, K.J.,Journal of Membrane Science, vol. 87, no. 1-2, pp. 35-46
Journal of Membrane Science, vol. 87, no. 1-2, pp. 35-46 1994
|Inhibition of Staphylococcus adherence to biomaterial by extracellular slime of S. epidermidis RP12 |
Giridhar, G.,Journal of Biomedical Material Research, vol. 28, no. 11, pp. 1289-1294
Journal of Biomedical Material Research, vol. 28, no. 11, pp. 1289-1294 1994
|Separation and size distribution of red blood cells of diverse size, shape and origin by flow/hyperlayer field-flow fractionation |
Barman, B. N. Ashwood, E. R. Giddings, J. C.
Anal. Biochem. v 212 IS- 1 pg 35-42 Jul 1993 1993
|Repetitive Batch as an Efficient Method for preparative Scale Enzymic Synthesis of 5-Azido-Neuraminic acid and 15N-L-Glutamic Acid |
Kragl, U.,Tetrahedron:Asymmetry; vol. 4, No. 6, 1193-1202
Tetrahedron:Asymmetry; vol. 4, No. 6, 1193-1202 1993
|Characterization and partial purification of carboxymethyl cellulase from Trichoderma reesei |
Yu, B.,Journal of Chinese Agriculural Chemical Society, vol.31, no. 5, pp. 614-622
Journal of Chinese Agriculural Chemical Society, vol.31, no. 5, pp. 614-622 1993
|Stimulator of proliferation of spleen colony-forming cells in acute sterile inflammation |
Milenkovic, P. et al.
Cell Proliferation, vol.26, no. 6, pp. 503-510 1993
|Purification and Characterization of a pullulan-hydrolyzing glucoamylase from Scletotium rolfsii |
Kelkar, H.S.; Starch, vol. 45, no. 10, pp. 361-368
Starch, vol. 45, no. 10, pp. 361-368 1993
|Purification and characterization of an endochitnase from Myrothecium verrucaria |
Vyas, P.,Journal of General Applied Microbiology, vol 39, pp. 91-99
Journal of General Applied Microbiology, vol 39, pp. 91-99 1993
|A partial characterization of a Sertoli cell-secreted protein stimulating Leydig cell testosterone production |
Murai T.; Noguchi K.; Nagamoto A.; Hosaka M. ; Endocrinologia Japonica 39/2 (209-215) 1992
Endocrinologia Japonica 39/2 (209-215) 1992 1992
|The use of anionic surfactants for reducing fouling of ultrafiltration membranes : their effects and optimization |
Chen et al. ,Journal of membrane science 1992 Vo- 67 Pg- 249-261
Journal of membrane science 1992 Vo- 67 Pg- 249-261 1992
|Effect of Transmembrane pressure pulsing on concentration polarization |
Rodgers, V.G.,Journal of Membrane Science, vol. 68, no. 1-2, pp. 149-168
Journal of Membrane Science, vol. 68, no. 1-2, pp. 149-168 1992
|Influence of temperature on the Ultrafiltration of silica sol in a stirred cell |
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|Removal of organic matter in BAC filters |
Merlet, N.,Journal of Water Science, vol.5, pp. 143-164
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|Antibody-affinity purification of novel a-L-fucosidase from mouse liver |
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|Are the Stirred Cells autoclavable?||Yes, stirred cells can be autoclaved at 121 C for 30 minutes. It is important, however, to tighten the base the loosen 1/4 turn before autoclaving. The tubing and the tubing connector are not autoclavable.|
|Are there cases where a stirred cell outperforms a crossflow type system?||Yes, at protein levels below 10 ug/ml. Also, pressure has a greater effect on flux as protein concentrations decrease. Most laboratory tangential flow systems operate at low pressure (20-25 psi), due to peristaltic pump limitations. Stirred cells do not require a pump for operation. They are normally pressurized to 55 psi.|
|Can I use compressed air instead of nitrogen to pressurize the cell?||We don't recommended it because air may cause pH shifts (C02 adsorption) or oxidation of product.|
|Can the membranes for the Stirred Cells be reused?||Yes, the membranes can be reused several times given they are used, cleaned, and stored properly. The operating instructions for the Ultrafiltration Membranes (literature number 99101) includes a restoration protocol for the membranes specific to each type. Also, under the operating procedures section is the proper method for usage and storage.|
|Can the Stirred Cell products be sanitized with Sodium Hypochlorite?||Yes. We recommend 100 ppm (parts per million) of Sodium Hypochlorite for 30 min.|
|Can the stirred cell be autoclaved with a membrane in place?||Yes, but only with the YM membrane. Put a small amount of buffer in the device to keep the membrane wet, tighten the bottom then loosen 1/4 turn. Remove any tubing and fittings, they are not autoclavable.|
|Do you sell parts for the Stirred Cells individually? What is the part number and price?||Yes, the parts lists for the Model 8000 Stirred Cells can be found in the Stirred Ultrafiltration User's Guide P99228. The parts list for the Model 2000 High Output Stirred Cell can be found in the user's guide P99109. Parts for the older style Stirred Cells (Model 12, 52, 202 or 402) are no longer being replaced.|
|Does tubing come with the stirred cell?||Yes, the following tubing comes with the stirred cell: a. Four feet of 1/4" pressure tubing for pressurizing the stirred cell. b. Four inches of permeate tubing.|
|How do I know the membrane in my stirred cells is in correctly?||The glossier side of the membrane should be facing up.|
|How do I remove the stirred cell after I've vented the pressure?||Push the cap down, then slide the cell out from the retaining stand.|
|How do I sterilize my stirred cell?||The 8000 series can be sterilized using any one of the following: 70% Ethanol 70% Isopropanol 5% Formaldehyde <200 ppm NaOCl (sanitize) The Model 2000 can be sterilized using either of the following: 5% Formaldehyde <200 ppm NaOCL (sanitize)|
|I bought some replacement bodies for my old style stirred cell. They did not fit properly. Has there been a product change?||In March of 1990 the stirred cell bases were changed to a white plastic molded part from a black metal part. Bodies compatible with the black metal base are not compatible with the white plastic base. When ordering a replacement body you will also need to order a new base. Here are the catalogue numbers for the affected products:
|I don't have any flow in my stirred cell. What happened?||a. Cell is not pressurized.
b. Membrane upside down. Glossy side should face up.
c. Plastic sheet not removed from membrane (XM-300 only),
d. Solution may be too concentrated (viscous).
e. Check that the membrane holder and filtrate port is not blocked.
|In what order do I assemble the parts to the tube fitting assembly?||Assemble in the following order: First, the hexagonal nut slides on to the red pressure tubing. The metal grab ring with flange facing away from hex nut fits into hex nut. The white plastic spacer with countersink surface towards grab ring is next. Finally, the o-ring is placed onto the end of the assembly.|
|What comes with the 8000 series stirred cells?||Everything except the magnetic stirrer, pressure source and the membrane.|
|What is the minimum volume that the stirred cells can concentrate to?||Amicon stirred cells can concentrate down to the minimum volumes listed below:
|Where can I order the filtrate tubing to the Stirred Cell (Cat. No. 4500430) when it is no longer offered at Millipore?||Cat. #4500430, TUBING (TYGON R-3603, 5/32X3/32), can be purchased through Fisher Scientific (800-766-7000) or Cole Palmer (800-323-4340)|
|Should I use the Ultracel regenerated cellulose membrane or the Biomax membrane in my TFF system?||It depends on the nature of your process volume and the process speed required. The Ultracel membrane is a lower protein binding membrane that should be used in low protein loading applications(<20 g/m2). The Biomax membrane is a faster flowing membrane that should be used in high protein loading applications (>20 g/m2). Ultracel is less tolerant to extreme pH conditions than the Biomax, but the Ultracel is more compatible with organic solvents.|
|What products and catalog numbers are required for the diafiltration set-up?||The diafiltration set-up requires the following:
1. Stirred Cell, for example catalogue number 5121 - Model 8010 (which uses 25mm membrane discs).
2. Magnetic stirring table.
3. Reservoir or dispensing pressure vessel. For example, catalogue number 6028 - RC800 Reservoir (800mL) or catalogue number XX6700P01 - 3.785 liter Dispensing Pressure Vessel (this will require relief valve, hose connectors, etc.)
4. CDS10 Concentration/Dialysis Selector, catalogue number 6003.
5. Pressure tubing to connect the system to a pressure source. XXPE00010 is 10 feet of 1/4" OD polyethylene tubing.
6. Pressure source
|What products should I purchase to continuously diafilter?||Pressurized Reservoir:
Model 8003 - RC800 (product no. 6028),
1/4" pressure tubing (product no. XXPE00010)
Concentration/Dialysis Selector CDS10 (product no. 6003)
|To what protein concentration can the solution be concentrated to using a stirred cell?||Practical limit is 10% by weight at room temp and 55psi. Slightly higher if solution can be warmed.|
|Which membranes should I use in my stirred cell?||The following list indicates the appropriate disc size for the various stirred cells listed below:
|What pressures are Stirred Cells operated at?||The maximum operating pressures for Models 8003, 8010, 8050, 8200 & 8400 is 75psi and Model 2000 is 100 psi. The recommended operating pressure for all Stirred Cells is 55 psi and <10psi when using a 100,000 MWCO membrane or greater.|
|What concentration can I use with the Stirred Cells?||All standard stirred cells operate within the following range:
|What could be wrong if the stirrer assembly in the Stirred Cell won't spin?||The stir bar won't spin unless the stirred cell is pressurized.|
|Stirred Ultrafiltration Cell User Guide|