Isopore™ Membrane Filters

Polycarbonate membrane for microscopy and visual analysis

Overview

Specifications

Ordering Information

White Membrane Filters · Catalogue No. Clear Sorting & Filtering
Catalog Numbericon Pore Size (µm)icon Filter Diameter (mm)icon Pack Sizeicon
ATTP013000.813100 Show Pricing & Availability
ATTP025000.825100 Show Pricing & Availability
ATTP037000.837100 Show Pricing & Availability
ATTP047000.847100 Show Pricing & Availability
ATTP142500.814250 Show Pricing & Availability
DTTP013000.613100 Show Pricing & Availability
DTTP025000.625100 Show Pricing & Availability
DTTP047000.647100 Show Pricing & Availability
DTTP142500.614250 Show Pricing & Availability
GTTP013000.213100 Show Pricing & Availability
GTTP025000.225100 Show Pricing & Availability
GTTP037000.237100 Show Pricing & Availability
GTTP047000.247100 Show Pricing & Availability
GTTP090300.29030 Show Pricing & Availability
GTTP142500.214250 Show Pricing & Availability
HTTP013000.413100 Show Pricing & Availability
HTTP025000.425100 Show Pricing & Availability
HTTP037000.437100 Show Pricing & Availability
HTTP047000.447100 Show Pricing & Availability
HTTP090300.49030 Show Pricing & Availability
HTTP142500.414250 Show Pricing & Availability
RTTP013001.213100 Show Pricing & Availability
RTTP025001.225100 Show Pricing & Availability
RTTP047001.247100 Show Pricing & Availability
RTTP142501.214250 Show Pricing & Availability
TCTP0130010.013100 Show Pricing & Availability
TCTP0250010.025100 Show Pricing & Availability
TCTP0470010.047100 Show Pricing & Availability
TCTP1425010.014250 Show Pricing & Availability
TETP013008.013100 Show Pricing & Availability
TETP025008.025100 Show Pricing & Availability
TETP047008.047100 Show Pricing & Availability
TETP090308.09030 Show Pricing & Availability
TETP142508.014250 Show Pricing & Availability
TMTP013005.013100 Show Pricing & Availability
TMTP025005.025100 Show Pricing & Availability
TMTP047005.047100 Show Pricing & Availability
TMTP090305.09030 Show Pricing & Availability
TMTP142505.014250 Show Pricing & Availability
TSTP013003.013100 Show Pricing & Availability
TSTP025003.025100 Show Pricing & Availability
TSTP047003.047100 Show Pricing & Availability
TSTP142503.014250 Show Pricing & Availability
TTTP025002.025100 Show Pricing & Availability
TTTP047002.047100 Show Pricing & Availability
VCTP013000.113100 Show Pricing & Availability
VCTP025000.125100 Show Pricing & Availability
VCTP047000.147100 Show Pricing & Availability
VCTP142500.114250 Show Pricing & Availability

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Brown Membrane Filters Clear Sorting & Filtering
Catalog Numbericon Pore Size (µm)icon Filter Diameter (mm)icon Pack Sizeicon
GTBP013000.213100 Show Pricing & Availability
GTBP025000.225100 Show Pricing & Availability
GTBP047000.247100 Show Pricing & Availability
HTBP013000.413100 Show Pricing & Availability
HTBP025000.425100 Show Pricing & Availability
HTBP047000.447100 Show Pricing & Availability

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    Documentation

    References

    Reference overview
    Urea transport in kidney brush-border membrane vesicles from an elasmobranch, Raja erinacea
    Robyn L. et al,J. experimental biology, 206, 3293-3302, 2003; internal ref BB04
    J. experimental biology, 206, 3293-3302, 2003; internal ref BB04 2003

    Comparison of microporous membrane morphologies using confocal scanning laser microscopy
    Charcosset C (reprint), et al.
    Journal of membrane science. 2000. v168, n2, p53-62 2000

    FAQ

    QuestionAnswer
    How do I clear my Millipore filters? It is possible to clear Millipore filters by choosing a clearing agent with the same refractive index as the membrane. This is called the PORE FILLING TECHNIQUE. Filters appear opaque because of the diffraction of light through the tortuous pores. Filling the pores with a liquid that has the same refractive index as the membrane (ex. xylene for MF filters) allows light to pass through the filter at a uniform speed thus rendering the filter transparent. This technique can be used for most membrane filters with a single refractive index. The refractive index for Durapore membrane is 1.42 and for MF membrane its 1.50.

    Please note that this does not work for polycarbonate membranes because these membranes have more than one refractive index (1.62 and 1.58). If desired, the porous structure can be dissolved from Isopore polycarbonates by dissolving the filter in chloroform, methylene chloride or 1-methyl-2 pyrrolidone.
    What is the blue separation paper between my filters? It is a parchmentalized paper specially formulated for Millipore Corporation.
    How do I tell the membrane from the separator? The blue paper is the separator.
    Which side of the membrane should I use, the shiny or dull side? Most researchers may not even notice that there is a "sidedness" to filters, and, for most applications, orientation will not affect filter performance. However, membranes do have a slightly asymmetric pore structure: the shiny side of the membrane is the "tighter" side. In some applications, you can take advantage of this difference by selecting a specific filter orientation. ultrafiltration membranes should always be used shiny side up, regardless of application for drop dialysis ( a buffer exchange technique in which a few drops of DNA or protein are placed in a 0.05 or 0.025 um filter and floated on a buffer solution), apply the sample to the shiny side of the filter and float the filter dull side to the buffer. This measure will enhance buffer exchange and discourage sample loss. The Millipore Express and Express Plus membranees are also sided - these membranes should be used shiny side facing down.
    What is the difference between pore size and pore size distribution? Whereas pore size is a measure of the diameter of the largest pore, pore size distribution is a measure of the range of pore sizes. The range of pore sizes can be normally distributed, and the spread can be quite narrow (e.g. the ratio of largest to smallest may be less than 2). On the other hand, pore size distribution can be very heterogeneous. In the case of large spreads and heterogeneity, the pore size will be far less predictive of flow rate (either filtration or capillary) than it will be for a membrane with a narrow pore size distribution. It is important to note that the pore size corresponding to the bubble point is not at the middle of the distribution, but is the largest pore.
    Is Polyvinylpyrolidone (PVP) toxic to cell growth on our Isopore membrane? PVP coated membranes are not suitable and will not support the attachment of cells to the membrane. PVP in Millicell-PC membranes has been validated for hybridoma cell growth and subsequent antibody production and found to be non-toxic. PVP is generally considered non-toxic and for a while was used in vivo as a plasma expander.

    C153Polycarbonate membrane for microscopy and visual analysis

    The Isopore membrane is a polycarbonate, track-etched screen filter recommended for all analyses in which the sample is viewed on the surface of the membrane. Isopore membrane offers distinct advantages for the analysis of airborne contaminants and other particles using optical or electron microscopy. The Isopore membrane is composed of polycarbonate film, which has a smooth, glass-like surface for clearer sample observation. The unique manufacturing process of the membrane ensures a precise pore diameter and a consistent pore size for accurate separation of samples by size. Isopore membranes do not stain resulting in low background interference. Clearing is not necessary for most transmitted light microscopy. Matched-weight filters are not usually required because of low, constant tar and ash weights. Isopore membranes are non-hygroscopic, which permits rapid drying and reduced sample analysis time.

    The Isopore™ membrane is a polycarbonate, track-etched screen filter recommended for all analyses in which the sample is viewed on the surface of the membrane. Isopore™ membrane offers distinct advantages for the analysis of airborne contaminants and other particles using optical or electron microscopy. The Isopore™ membrane is composed of polycarbonate film, which has a smooth, glass-like surface for clearer sample observation. The unique manufacturing process of the membrane ensures a precise pore diameter and a consistent pore size for accurate separation of samples by size. Matched-weight filters are not usually required because of low, constant tar and ash weights.

    Features & Benefits

    • Membrane structure retains particles on the surface, simplifying counting and analysis
    • Isopore™ membranes do not stain, resulting in low background interference
    • Non-hygroscopic, allowing for rapid drying and reduced sample analysis time
    • Translucent material does not require clearing for transmitted light microscopy; also available in brown variety

    Applications

    Air Monitoring, Epifluorescent Microscopy, Chemotaxis Assays

    Product Performance

    For more information visit: www.emdmillipore/filterdiscs

    General Specifications

    Color: white or brown**
    Surface: plain
    Wettability: hydrophilic
    Thickness: 7–22 µm
    Porosity: 5–20%
    Sterilization: by autoclave (121 °C at 1 bar), EO or gamma*
    Gravimetric extractables: < 1.0%

    * Brown color may fade if autoclaved.
    ** Brown membranes are compatible with immersion oils, including Type B and Type F.

    Detailed Specifications
    Applications Filter Code* Color Pore Size
    (µm)
    Bubble Point
    (bar)
    Water Flow Rate
    (mL/min/cm2)
    Air Flow Rate
    (L/min/cm2)
    Chemotaxis, bioassays, cytology, air monitoring VMTP White 0.05 7.1 0.35
    VCTP White 0.1 7.1 0.5 1.5
    Chemotaxis, bioassays, cytology, air monitoring, SEM analysis, sterility testing GTTP White 0.2 3.5 6 1
    Epifluorescent microscopy, particle monitoring, air monitoring GTBP Brown 0.2 3.5 6
    Adsorbable organic halides (AOX), air monitoring, particle monitoring HTTP White 0.4 1.5 50 7
    Epifluorescence microscopy, particle monitoring, air monitoring HTBP Brown 0.4 2 18
    Reflective light microscopy, SEM analysis, gravimetric analysis, air monitoring DTTP White 0.6 0.6 25 10
    Reflective light microscopy, SEM analysis, gravimetric analysis, air monitoring, asbestos monitoring ATTP White 0.8 0.6 40 10
    Chemotaxis, bioassays, cytology, air monitoring RTTP White 1.2 0.6 110 20
    TTTP White 2 0.3 90 22
    TSTP White 3 0.05 180 50
    Parasitology, chemotaxis, bioassays, cytology, air monitoring TMTP White 5 250 50
    Chemotaxis, bioassays, cytology, air monitoring TETP White 8 250 60
    TCTP White 10 250 69
    TKTP White 12 250 127
    *Corresponds to first 4 digits of catalogue number