Millicell® 24 & 96-Well Cell Culture Insert Plates

Membrane-based culture for optimum growth and differentiation

Overview

Specifications

Ordering Information

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Millicell-24 well plates & accessories Clear Sorting & Filtering
Catalog Numbericon Descriptionicon Componentsicon Qty/Pkicon
PSET010R1Millicell-24 Cell Culture Insert Plate, polycarbonate, 8.0 µm24-well cell culture plate, single-well feeder tray, 24-well receiver tray, lid1 Show Pricing & Availability
PSET010R5Millicell-24 Cell Culture Insert Plate, polycarbonate, 8.0 µm24-well filter plate, 24-well receiver plate, lid5 Show Pricing & Availability
PSHT010R1Millicell-24 Cell Culture Insert Plate, polycarbonate, 0.4 µm24-well cell culture plate, single-well feeder tray, 24-well receiver tray, lid1 Show Pricing & Availability
PSHT010R5Millicell-24 Cell Culture Insert Plate, polycarbonate, 0.4 µm24-well cell culture plate, single-well feeder tray, lid5 Show Pricing & Availability
PSMT010R1Millicell-24 Cell Culture Insert Plate, polycarbonate, 5.0 µm24-well cell culture plate, single-well feeder tray, 24-well receiver tray, lid1 Show Pricing & Availability
PSMT010R5Millicell-24 Cell Culture Insert Plate, polycarbonate, 5.0 µm24-well filter plate, 24-well receiver plate, lid5 Show Pricing & Availability
PSMW010R524-well Receiver Tray with Lid5 Show Pricing & Availability
PSRP010R1Millicell-24 Cell Culture Insert Plate, polyethylene terephthalate, 1.0 µm24-well cell culture plate, single-well feeder tray, 24-well receiver tray, lid1 Show Pricing & Availability
PSRP010R5Millicell-24 Cell Culture Insert Plate, polyethylene terephthalate, 1.0 µm24-well cell culture plate, single-well feeder tray, lid5 Show Pricing & Availability
PSST010R1Millicell-24 Cell Culture Insert Plate, polycarbonate, 3.0 µm24-well cell culture plate, single-well feeder tray, 24-well receiver tray, lid1 Show Pricing & Availability
PSST010R5Millicell-24 Cell Culture Insert Plate, polycarbonate, 3.0 µm24-well filter plate, 24-well receiver plate, lid5 Show Pricing & Availability
PSSW010R5Single-well Receiver Tray with Lid5 Show Pricing & Availability

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Millicell-96 well plates & accessories Clear Sorting & Filtering
Catalog Numbericon Descriptionicon Componentsicon Qty/Pkicon
MACAC0RS596-Well Feeder/Transport Tray with Lid96-well transport tray(5), lid(5)5 Show Pricing & Availability
PSHT004R1Millicell-96 Cell Culture Insert Plate, polycarbonate, 0.4 µm96-well cell culture plate(1), Single-well feeder tray(1), 96-well receiver tray(1), lid(1)1 Show Pricing & Availability
PSHT004R5Millicell-96 Cell Culture Insert Plate, polycarbonate, 0.4 µm96-well cell culture plate(5), single-well feeder tray(5), lid(5)5 Show Pricing & Availability
PSHT004S5Millicell-96 Cell Culture Insert Plate, polycarbonate, 0.4 µm96-well cell culture plate(5), 96-well receiver tray(10), lid(5)5 Show Pricing & Availability
PSRP004R1Millicell-96 Cell Culture Insert Plate, polyethylene terephthalate, 1.0 µm96-well cell culture plate, single-well feeder tray, 96-well receiver tray, lid1 Show Pricing & Availability
PSRP004R5Millicell-96 Cell Culture Insert Plate, polyethylene terephthalate, 1.0 µm96-well cell culture plate, single-well feeder tray, lid5 Show Pricing & Availability

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Common accessories Clear Sorting & Filtering
Catalog Numbericon Descriptionicon Qty/Pkicon
MERS00002Millicell ERS-2 Voltohmmeter1 Show Pricing & Availability
MERSSTX01Replacement Electrodes, 1 pair1 Show Pricing & Availability

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    Documentation

    Brochure

    Title
    Advanced cell culture systems for every challenge.
    Multiwell solutions for discovery research and sample prep
    Sterile filtration you can trust.(EMD)

    Data Sheet

    Title
    Millicell Cell Culture Plate Inserts: For attachment-dependent, suspension cell or organotypic growth with apical and basolateral access

    References

    Reference overviewApplication
    Propagation of human embryonic stem cells in a microporous membrane-based indirect co-culture system
    Kelsey Albert, Steven Sheridan, Louise Laurent, Igor Ulitsky, Ron Shamir, Jeanne Loring, & Raj R. Rao
    Biochemical and Biophysical Research Communications 2010

    Effects of cimetidine on the biological behaviors of human gastric cancer cells
    Jiang CG, Liu FR, Xu HM, Wu T, Gao J.
    Zhonghua Yi Xue Za Zhi. 2006 Jul 11;86(26):1813-6 2006

    Overexpression of TIMP-1 mediated by recombinant adenovirus in hepatocellular carcinoma cells inhibits proliferation and invasion in vitro
    Xia D, Yan LN, Xie JG, Tong Y, Yan ML, Wang XP, Zhang MM, Zhao LY.
    Hepatobiliary Pancreat Dis Int. 2006 Aug;5(3):409-15. 2006

    Glucose utilization by the retinal pigment epithelium: evidence for rapid uptake and storage in glycogen, followed by glycogen utilization.
    Senanayake P, Calabro A, Hu JG, Bonilha VL, Darr A, Bok D, Hollyfield JG.
    Exp Eye Res. 2006 Aug;83(2):235-46. Epub 2006 May 11. 2006

    Cell Culture
    A comparison of polarized and non-polarized human endometrial monolayer culture systems on murine embryo development.
    Baghaban Eslami Nejad MR, Rezazadeh Valojerdi M, Kazemi Ashtiani S.
    J Exp Clin Assist Reprod. 2005 Apr 19;2(1):7. 2005

    Cell Culture

    User Guides

    Title
    Millicell 24 and Millicell 96
    Millicell Culture Plate Inserts

    C10584Membrane-based culture for optimum growth and differentiation

    Millicell cell culture insert plates are a patented device designed to support cell growth and differentiation of endothelial and epithelial cell lines, including CaCo-2.

    The Millicell device is available with a 0.4 µm PCF and a variety of PET membrane pore sizes. The plate is designed for maximum user convenience and incorporates features such as apical assist for easier pipetting and basolateral access. To reduce the risk of monolayer contamination, the membrane plates have feet to elevate the plate above the work surface when disassembled from the feeder tray. Tear drop-shaped receiver wells eliminate air bubbles as plates are assembled.

    Millicell plate are optimized to grow and sustain high integrity cell monolayers. Cells grown on Millicell plate membranes grow better than on plastic because the cells are nourished from both the apical and basolateral sides. Cell growth and function more closely mimic in vivo. Millicell plates are designed for analysis as well as cell growth and can be used manually or with automated cell seeding, feeding and washing systems

    With this complete system, you can grow, feed, and analyze cells in one membrane-bottom plate. The plate can be used with either a single-well or a multi-well feeding tray. At the time of transport analysis, the plate is simply transferred to a multi-well transport tray for analysis.

    This streamlined design enhances functionality with:

    • Seed-and-feed systems
    • Automated liquid handling systems and span pipettes
    • Basolateral and apical access to cells
    • Transepithelial electrical resistance (TEER) measurement systems

    Design improves cell culture assays and analysis

    Design improves cell culture assays and analysis

    In-plate differentiation of embryonic stem cells and phenotype analysis

    Neuron differentiation of embryonic stem cells in Millicell 24 1 µm PET filter plates. Murine embryonic stem cells were formed into suspended embryoid bodies (EBs) then transferred to 1 µm PET Millicell 24 for attachment and differentiation. Neural differentiation after retinoic acid treatment of attached EBs was confirmed by anti-neurofilament immunofluoresence. (Insert: Inverted phase contrast imaging through membrane of live EBs in media.)

    Neuron differentiation of embryonic stem cells in Millicell 24 1 µm PET filter plates. Murine embryonic stem cells were formed into suspended embryoid bodies (EBs) then transferred to 1 µm PET Millicell 24 for attachment and differentiation. Neural differentiation after retinoic acid treatment of attached EBs was confirmed by anti-neurofilament immunofluoresence. (Insert: Inverted phase contrast imaging through membrane of live EBs in media.)

    Demonstrated transport and efflux studies with integral differentiated Caco-2 monolayers

    Differentiation of 21-day Caco-2 monolayer by efflux pump expression. Anti-MDR1 immunohistochemistry confirms p-glycoprotein (MDR1) expression of Caco-2 monolayer grown in Millicell 24 with 1 µm PET membrane.

    Differentiation of 21-day Caco-2 monolayer by efflux pump expression. Anti-MDR1 immunohistochemistry confirms p-glycoprotein (MDR1) expression of Caco-2 monolayer grown in Millicell 24 with 1 µm PET membrane.

    Feature Benefit
    High pore density 0.4µm PCF membrane Grows well differentiated Caco-2 cells with no grow through
    1.0µm Transparent PET membrane Allows for visualization of live cells using microscopy
    Apical and basolateral access holes Ease of sample access when performing sequence time transport analysis
    Apical assist channel Prevent monolayer disruption and membrane damage while performing manual assays
    HTS Format
    Filter plate has feet Do not compromise sterility if set on bench

    Choice of membranes lets you optimize for your application

    Millicell cell culture plates can be used for a wide variety of applications from cell surface receptor assays, to in vitro toxicology. The device can also be used to measure drug transport and other cell based assays. See the applications guide for a complete listing.

    The plates are available with thin-film polycarbonate (PCF) membrane or polyester (PET), which is a microscopically-transparent membrane.

    Millicell® plates are optimized to grow and sustain high integrity cell monolayers. Cells grown on Millicell® plate membranes grow better than on plastic because the cells are nourished from both the apical and basolateral sides. Cell growth and function more closely mimic what occurs in vivo. Millicell® plates are designed for analysis as well as cell growth and can be used manually or with automated cell seeding, feeding and washing systems.

    Features & Benefits

    • Compatible with seed-and-feed systems
    • Works with automated liquid handling systems and span pipettes
    • Basolateral and apical access to cells
    • Transepithelial electrical resistance (TEER) measurement systems available

    Applications

    Cell Growth, Feeding, Cell Analysis

    For more information visit: www.emdmillipore.com/cellculture