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Technical Info and Required Licenses
|Millicell Organotypic Cell Culture Procedure|
|Millicell Cell Culture Plate Inserts: For attachment-dependent, suspension cell or organotypic growth with apical and basolateral access|
|Millicell Inserts and Plates|
References | 55 Available | See All References
|Propagation of human embryonic stem cells in a microporous membrane-based indirect co-culture system |
Kelsey Albert, Steven Sheridan, Louise Laurent, Igor Ulitsky, Ron Shamir, Jeanne Loring, & Raj R. Rao
Biochemical and Biophysical Research Communications 2010
|Astrocyte growth effects of vascular endothelial growth factor (VEGF) application to perinatal neocortical explants: Receptor mediation and signal transduction pathways |
Nina Mani, Alfia Khaibullina, Janette Krum and Jeffrey Rosenstein
Experimental Neurology 192 (2005); 394-406 2005
|Subcellular localisation of recombinant a and g-synuclein |
Christian Specht, Cezar Tigaret, George Rast, Agnea Thalhammer, York Rudhard and Ralf Schoepfer
Mol. Cell. Neurosci., 28 (2005); 326-334 2005
|Establishment of the organotypic model of amyotrophic lateral sclerosis from the SD rats' spinal cord |
Diao ZY, et. al,Beijing Da Xue Xue Bao. 2005 Apr 18;37(2):134-8. Chinese.
Beijing Da Xue Xue Bao. 2005 Apr 18;37(2):134-8. Chinese. 2005
|Neural stem cells protect against glutamate-induced excitotoxicitiy and promote survival of injured motor neurons through the secretion of neurotrophic factors |
Jeronia Llado, Christine Haenggeli, Nicholas Maragakis, Evan Snyder and Jeffrey Rothstein
Mol. Cell. Neurosci. , 27 (2004); 322-331 2004
|Development of an in vitro blood-brain barrier model-cytotoxicity of mercury and aluminum. |
Toimela, T et. al.,Toxicol Appl Pharmacol. 2004 Feb 15;195(1):73-82.
Toxicol Appl Pharmacol. 2004 Feb 15;195(1):73-82. 2004
|Morphological differentiation of bone marrow stromal cells into neuron-like cells after co-culture with hippocampal slice |
Abouelfetouh, Ayman, et al
Brain Research (2004), Volume 1029, Issue 1 pp 114-119 2004
|Effect of sodium bicarbonate on extracellular pH, matrix accumulation, and morphology of cultured articular chondrocytes. |
Waldman SD, Couto DC, Omelon SJ, Kandel RA
Tissue Engineering (2004), Nov-Dec;10(11-12):1633-40 2004
|FGF-10 plays an essential role in the growth of the fetal prostate. |
Annemarie A. Donjacour, Axel A. Thomson and Gerald R. Cunha
Developmental Biology 261 (1): 39-54 2003
|Changes in lymphokine receptor expression and fatty acid composition of phospholipids and triacylglycerols in rat adipocytes associated with lymph nodes following a transient immune challenge. |
J. D. Priddle, C. A. Mattacks, D. A. Sadler, H. A. MacQueen and C. M. Pond
Cell Biology International 27 (1): 23-29 2003
|Neuroprotective effects of anticonvulsants in rat hippocampal slice cultures exposed to oxygen/glucose deprivation. |
Jens C. Rekling,Neuroscience Letters 335 (3): 167-170
Neuroscience Letters 335 (3): 167-170 2003
|Identification of the laminar-inducing factor: Wnt-signal from the anterior rim induces correct laminar formation of the neural retina in vitro. |
Shinichi Nakagawa, Shinji Takada, Ritsuko Takada and Masatoshi Takeichi
Developmental Biology 260 (2): 414-425 2003
|Cell Based Assays|
|Signals from the cerebellum guide the pathfinding of inferior olivary axons. |
Yan Zhu, Khurram Khan and Sarah Guthrie; Developmental Biology 257 (2): 233-248
Developmental Biology 257 (2): 233-248 2003
|Adenovirus-mediated G[alpha]q-protein antisense transfer in neurons replicates G[alpha]q gene knockout strategies. |
F. C. Abogadie, R. Bron, S. J. Marsh, L. J. Drew, J. E. Haley, N. J. Buckley, D. A. Brown and P. Delmas; Neuropharmacology 42 (7): 950-957
Neuropharmacology 42 (7): 950-957 2002
|Fibroblasts and ascorbate regulate epidermalization in reconstructed human epidermis. |
Suk Wha Kim, Il-Whan Lee, Hyun-Joo Cho, Kwang-Hyun Cho, Kyu Han Kim, Jin-Ho Chung, Peter I. Song and Kyoung Chan Park
Journal of Dermatological Science 30 (3): 215-223 2002
|Preparation and characterization of polyalkene membranes modified with four different ion-exchange groups by radiation-induced graft polymerization. |
Kwang-Pill Lee, Seong-Ho Choi and Hee-Dong Kang
Journal of Chromatography A 948 (1-2): 129-138 2002
|Ultrafilter co-culture, a new metehod for estimating the molecular mass of bioactive substances,indicates a small molecule neurotrophic substance is released from cultured cerebellar granule neurons of the BALB/c mouse |
Naoto Fujikawa ,Brain Research, 947, 243-251, 2002, internal ref Cult-01
Brain Research, 947, 243-251, 2002, internal ref Cult-01 2002
|Origin of GABAergic neurons in the human neocortex |
K. Letinic, R. Zoncu and P. Rakic
Nature 417 (2002), pp. 645–649 2002
|Organotypic rat cerebellar slice culture as a model to analyze the molecular pharmacology of GABAA receptors. |
Eugen Davids, Wulf Hevers, Kerstin Damgen, Kehong Zhang, Frank I. Tarazi and Hartmut Luddens
European Neuropsychopharmacology 12 (3): 201-208 2002
|Morphological study of fully and partially isolated early human follicles. |
Ronit Abir, Benjamin Fisch, Shmuel Nitke, Elimelech Okon, Ahud Raz and Zion Ben Rafael
Fertility and Sterility 75 (1): 141-146 2001
|Characterization of nucleus pulposus-like tissue formed in vitro. |
Yongliang Sun, Mark Hurtig, Robert M. Pilliar, Marc Grynpas and Rita A. Kandel
Journal of Orthopaedic Research 19 (6): 1078-1084 2001
|A cell culture medium that supports the differentiation of human retinal pigment epithelium into functionally polarized monolayers. |
Hu, J and Bok, D
Mol. Vis., 7: 14-9 (2001) 2001
PURPOSE: The retinal pigment epithelium (RPE) in vivo is known to have polarized membrane domains that are essential for its normal function. Unless the proper cell culture conditions are used, these polarized features are often lost. In the past, the use of Chee's Essential Medium (CEM) in our RPE cultures has produced functional polarity of the cell monolayers. Unfortunately, except by custom formulation, which is costly, this product is no longer commercially available. We therefore sought to develop a replacement culture medium that would support morphological and functional polarity of RPE membrane domains when the cells are removed from the in vivo milieu. METHODS: To test the performance of this CEM replacement medium in comparison with three other culture media, we grew fetal human RPE to confluence on Millipore Millicell culture wells. We then used Na,K ATPase as a membrane domain marker by displaying it with polyclonal antibodies. This marker was chosen because it is not always properly polarized in culture. Immunofluorescence was imaged by laser confocal microscopy of whole mounted intact monolayers on their Millicell supports. We also used transepithelial resistance (TER) as a measurement of functional polarity as well as bestrophin protein expression as an index of cell differentiation. The expression of Na,K ATPase and bestrophin was confirmed by Western blot analysis of whole RPE cell extracts. RESULTS: Immunofluorescence labeling of cultured RPE Na,K ATPase was observed exclusively on the apical membrane when the CEM replacement or DMEM with high glucose was used. However Na,K ATPase was not completely polarized in DMEM/F12 medium and the cells did not express detectable Na,K ATPase in DMEM with low glucose. Western blots showed that Na,K ATPase was expressed at similar levels in CEM replacement, DMEM with high glucose and DMEM/F12 as indicated by the intensity of an approximately 100 kDa band representing the a subunit. The CEM replacement gave superior TERs as well, ranging from about 2 to 5.6 fold higher than the other media. Bestrophin protein was readily detectable by Western blot in CEM replacement medium whereas it was barely detectable in DMEM/F12 and undetectable in DMEM with high and low glucose. CONCLUSIONS: We have provided immunocytochemical evidence that the CEM replacement medium supports the appropriate membrane domain expression of Na,K ATPase when the cells are grown on Millicell chambers. Excellent TERs and robust expression of bestrophin are also observed. This combination of features was not observed when other, standard culture media were used. The results suggest that, under these conditions, cultured human RPE develops a highly differentiated and functional polarity appropriate for the in vitro modeling of RPE in vivo function.
|Basic fibroblast growth factor induces primordial follicle development and initiates folliculogenesis. |
Eric Nilsson, Jeff A. Parrott and Michael K. Skinner
Molecular and Cellular Endocrinology 175 (1-2): 123-130 2001
|Biodegradable three-dimensional networks of poly(dimethylamino ethyl methacrylate: Synthesis, characterization and in vitro studies of structural degradation and cytotoxicity. |
Monique J. Bruining, Harriet G.T. Blaauwgeers, Roel Kuijer, Elisabeth Pels, Rudy M. M. A. Nuijts and Leo H. Koole
Biomaterials 21 (6): 595-604 2000
|Composition and immunofluorescence studies of biliary sludge in patients with cholesterol or mixed gallstones. |
Paulette Lechene de la Porte, Huguette Lafont, Nicole Domingo, Gunther Meyer, Iris Muller, Benedikta Zundt and Dieter Jungst; Journal of Hepatology 33 (3): 352-360
Journal of Hepatology 33 (3): 352-360 2000
|Long-term maintenance of mature hippocampal slices in vitro. |
Zhongmin Xiang, Sabina Hrabetova, Shaye I. Moskowitz, Patrizia Casaccia-Bonnefil, Steven R. Young, Volker C. Nimmrich, Henri Tiedge, Stephen Einheber, Sergei Karnup, Riccardo Bianchi and Peter J. Bergold
Journal of Neuroscience Methods 98 (2): 145-154 2000
|A new skin equivalent model: dermal substrate that combines de-epidermized dermis with fibroblast-populated collagen matrix. |
Dong-Youn Lee, Hi-Tae Ahn and Kwang-Hyun Cho
Journal of Dermatological Science 23 (2): 132-137 2000
|The Suprchiasmatic Nucleus in Stationary Organotypic Culture. |
M. Belenky, S. Wagner, et al
Neuroscience 70 (1): 127-143 1996
|In vitro measurement of drug transport using a new diffusion chamber compatible with Millicell culture supports: performance with Caco-2 monolayers. |
Kuhfeld MT, Stratford, RE
International Journal of Pharmaceutics 133 (1996) 47-58 1996
|A strategy for the analysis gene expression during neural development. |
Donald Arnold, Lei Feng, Jae Kim, and Nathaniel Heintz
Neurobiology 92 9970-9974 1994
|Structural modifications associated with synaptic development in area CA1 of rat hippocampal organotypic cultures. |
L. Stoppini and D. Muller P.-A. Buchs
Developmental Brain Research 71 81-91 1993
|Measurement of free desipramine in serum by ultrafiltration with immunoassay |
Hursting, M.J. et al.
Clinical Chemistry, 1992, V38, N12, pg. 2468-2471 1992
|Characterization of corneal endothelium cell cultured on microporous membrane filters. |
Geroski, DH and Hadley, A.
Curr. Eye Res. (1992) Jan:11(1):61-72 1992
|Editing of apolipoprotein B messenger RNA in differentiated Caco-2 cells |
Jiao S, Moberly JB, Schonfeld G.
J Lipid Res. 1990 Apr;31(4):695-700 1990
|Growth and Characterization of Polarized Monolayers Epididymal Epithelial Cells and Sertoli Cells in Dual Environment Culture Chambers |
Byers, S.W., Hadley, M.A., Djakiew, D., and Dym, M.
Journal of Andrology, Vol, 7, pp. 59-68 1986
|Epithelial-Mesenchymal Interactions: Effects of a Dental Biomatrix on Odontoblasts |
Cam, Y., Meyer, J.M., Staubli, A., and Ruch, J.V
Arch. Anat. Micr. Morphol, Exper., vol, 75, pp, 75-89 1986
|Kinin Effects on Electrogenic Ion Transport in Primary Culture of Pig Renal Papillary Collecting Tubule Cells |
Cuthbert, A.W., Geroge, A.M., and MacVinish, L; Am. J. Physio., Vol 249, pp. F439-F447
Am. J. Physio., Vol 249, pp. F439-F447 1985
|A Study of the Adhesive, Locomotory and and Invasive Behaviour of Walker 256 Carcinosarcoma Cells |
Elvin, P., Wong, V., and Evans, C.W.
Expl. Cell. Bio, Vol. 53, pp. 9-18 1985
|Difference in the Arginine Vasopressin Responsive cAMP Production Between Apical and Basolateral Membrane Cultured Renal Epithelial Cells (MDSK) |
Kinoshita, Y., Fukase, M., Takenaka, M., Nakada, M., and Fujita, T.; Endrocrinol. Japon., Vol 32 (3), pp. 413-420
Endrocrinol. Japon., Vol 32 (3), pp. 413-420 1985
|Localization of Collagen Types IV and V, Laminin, and Heparan Sulfate Proteoglycan to the Basal Lamina of Kidney Epithelial Cells in Transfilter Metanephric Culture |
Bonadio, J.F., Sage, H., Cheng, F. Bernstein, J. and Striker, G.E.
Am. J. Pathology, Vol. 116, pp. 289-296 1984
|Vesicular Stomatitis Virus Infects and Matures Only Through the Basolateral Surface of the Polarized Epithelial Cell Line, MDCK |
Fuller, S., von Bonsdorff, C.-H., and Simons, K.
Cell, Vol. 38, PP. 65-77 1984
|Apical-Basolateral Membrane Asymmetry in Canine Cortical Collecting Tubule Cell |
Garcia-Perez, A. and Smith, W.L.
J. Clin. Invest., Vol. 74, pp. 63-74 1984
|Epithelial Properties of Human Colonic Carcinoma Cell Line Caco-2: Electrical Parameters |
Grasset, E., Pinto, M. Dussaulx, E., Zweibaum, A., and Desjeux, J.-F.
American Journal of Physiology, Vol. 247, pp. C260-C267 1984
|Factor Affecting the Differentiation of Epithelial Transport and Responsiveness to Hormones |
Handler, J., Preston, A.S., and Steel, R.E.
Federations Proceedings, Vol. 43, pp. 2221-2224 1984
|Sorting of Apical Plasma Membrane Glycoprotein Occurs Before It Reaches the Cell Surface in Cultured Epithelial Cells |
Matlin, K.S., and Simons, K.
J. Cell Biol., Vol 99, pp. 2131-2139 1984
|Biogenesis of Epithelial Cell Polarity: Intracellular Sorting and Vectorial Exocytosis of an Apical Plasma Membrane Glycoprotein |
Misek, D.E., Bard, E., and Rodriguez-Boulan, E.
Cell, Vol 39., pp. 537-546 1984
|Analysis of Epithelial Cell Surface Polarity with Monoclonal Antibodies |
Ojakian, G.K., and Herzlinger, D.A.
Federation Proceedings, Vol 43(8), pp. 2208-2216 1984
|The Action of Epinephrine on Madin-Darby Canine Kidney Cells |
Simmons, N.L., Brown, C.D.A., and Rugg, E.L.
Federation Proceedings, Vol. 48(8), pp. 2225-2229 1984
|Use of Cultured Epithelia to Study Transport and Its Regulation |
Handler, J.S.; J. Exp. Biol., Vol 106, pp. 55-69
J. Exp. Biol., Vol 106, pp. 55-69 1983
|Epithelial Permeability and the Transepithelial Migration of Human Neutrophils |
Milks, L.C., Brontoli, M.J. and Cramer, E.B.
J. Cell Biol., Vol. 96, pp. 1241-1247 1983
|Transepithelial Transport by Pulmonary Alveolar Type II Cells in Primary Culture |
Mason, R.J., Williams, J.H., Widdicombe, M.J., Sanders, D.S. and Berry, L.C.
Proc. Nat'l Acad. Sci. USA, Vol 79, pp. 6033-6037 1982
|Transepithelial Transport in Cell Culture: D-Glucose Transport by a Pig Kidney Cell Line (LLC-PK1) |
Misfledt, D.S., and Saunders, M.C.
Journal of Membrane Biology, Vol 39, pp. 13-18 1981
|Tumor Promoter-Induced Changes in the Permeability of Epithelial Cell Tight Junctions |
Ojakian, G.K.,Cell, Vol. 23, pp. 95-103
Cell, Vol. 23, pp. 95-103 1981
|Transepithelial Migration of Human Neutrophils: An In Vitro Model System |
Cramer, E.B., Milks, L.C. and Ojakian, G.K.
Proc. Nat'l Aca. Sci. USA, Vol. 77(7), pp. 4069-4073 1980
|Extended Expression of Differentiated Function in Primary Cultures of Adult Liver Parenchymal Cells Maintained on Nitrocellulose Filters |
Savage, C.R. and Bonney, R.J.
Exp. Cell Res., Vol 114, pp. 307-315 1978
|Are the Millicell PCF inserts tissue culture treated on both sides of the membrane?||Yes, they are tissue culture treated on both sides of the membrane. This allows for attachment- dependent cell culture on both sides of the membrane.|
|Are the Millicell units sterile and, if so, how are they sterilized?||The Millicell units are sold sterile in individual blister packs. The HA and CM units are ETO sterilized and the PC and PCF are Gamma Irradiated.|
|Can cells grown on Isopore (polycarbonate) membrane be viewed under a microscope?||Yes, we can observe the cells seeding on the membrane of pore size 1 um.|
|Can I make membrane potential measurements with the Millicell-ERS?||Yes, membrane potential measurements can be made with the ERS. Short circuit potential, however, cannot be done.|
|How can I be sure that I have a confluent monolayer in my Millicell ?||There are several answers to this question based on the type of membrane you are using and the equipment you have. It is possible to view, via phase contrast microscopy, actual cell layers if you are using Millicell HA, PC and PCF.
With Millicell CM you can use bright field microscopy as well to check for confluency. Also, one way of assuring confluency is to seed your cells at optimal levels. Most cell lines do best at a density of 5x10^5 to 1x10^6 cells/ cm2. So long as you are working with a cell type (i.e. epithelial cells) that exhibits resistance due to membrane potential you can also use the Millicell ERS to confirm confluency.
|How long do the electrodes last?||The electrodes will last at least 6 months with normal use and care, barring any physical damage. To prolong life of the electrodes, clean after every use and store dry.|
|How should I store electrodes when not in use?||When storing electrodes for long periods of time, wash the electrodes with Milli-Q water or equivalent to remove salts and proteins, then store dry. For short term storage electrodes can be stored in a buffered solution. Make sure the electrode cable plug is connected to the electrode port on the Millicell ERS meter so that the system is internally short-circuited and electrode symmetry is maintained.|
|I am coating the Millicell CM with Rat Tail collagen according to your instructions and sometimes I am seeing an empty ring at the periphery of the insert where the collagen has not filled in and an empty "hole" at the center where there is no coating either. How can I avoid this from happening in the future?||If you are using Type 1 collagen, it should be filter sterilized in non-denaturing alcohol prior to use in the Millicell. Also, try looking at the insert while you are coating it and tap/rotate it to enhance uniform liquid coating. The entire membrane should go clear which indicates uniform wetting/coverage of the Millicell. Also, be sure to use at least minimum volumes of 50ul for the 12mm and 350 ul per 30 mm.|
|I am concerned about the possibility of introducing surfactants in cell cultures using the Millicell PCF product. Is the Polycarbonate membrane in Millicell-PCF PPVP (surfactant) free?||Yes, the Polycarbonate membrane in Millicell-PCF is PPVP (surfactant) free.|
|I am culturing tissue slices on the Millicell Polycarbonate unit (PIHP01250) and it appears that the media is not being retained by the unit. What is going on?||The 0.4 um PCF polycarbonate membrane is tissue culture treated and, therefore, hydrophilic. In long-term culture ( >than a few to 24 hours) they will NOT retain fluid since it will slowly pass through the pores. A monolayer of epithelial cells on the membrane serves as a secondary barrier which will selectively prevent/or allow molecules to pass.
If you are referring to an overnight culture with no media outside the Millicell this result is to be expected. If there is equal fluid heights both inside and outside the Millicell ( ~ 400 ul inside and 600 ul outside) then the fluid will be retained. Since they are doing organotypic culture you could easily adjust the inside and outside volumes to the same desire height to make sure the culture remains bathed in media.
|I am testing my Millicell ERS and cannot get a value when I take a reading. What am I doing wrong ?||When testing the unit, it is very important to hold down the test button until the reading is taken, otherwise you will not get a real value. If you only push the button down and release no reading will be obtained.|
|I have seeded cells on the Millicell and they are detaching from the membrane, what do I do to correct this?||Make sure that the medium on the outside of the insert is lower than the level of medium on the inside of the insert. Also, if it is important to remember that certain cells lines, i.e. attachment dependent cells, often require pretreatment of the Millicell membrane in order to enhance attachment. This is usually accomplished through ECM coating...especially in the case of Millicell CM where coating is always required. Too much feeding of unconditioned media can also cause detachment in some cell types, e.g. caco-2.|
|Is it necessary to treat the Millicell CM with an ECM coating if I am doing organotypic culture ?||In general, it is not required that you treat the Millicell CM with an ECM coating when doing organotypic culture but this is dependent on the type of tissue being cultured and the procedure being followed. In Tech Note TN062 , we outline a procedure for organotypic culture that uses a pretreatment of the Millicell CM with poly-ornithine ( an attachment factor). Other references cited in this paper do not use this precoating procedure. When evaluating whether or not to use an attachment factor in your experiment, it is best to use as many resources as possible and decide based upon the specific experimental parameters in your situation.|
|Is there a protocol to cut out the membranes from Millicell inserts?||The membrane can be removed from the insert insert. This is most easy accomplished using a Diamond knife over a piece of cork.|
|What are the dimensions of the Millicell units?||For the 12 mm units, the height is 10.5 mm. The diameter (0D) of the base is 13mm & the top is 12 mm. The ID is 10 mm. The membrane diameter is 12mm and effective membrane area is 0.6 cm2. For the 30 mm unit, the dimensions are 13 mm height. 32mmOD base, 30 mmOD top and 27mmID. The membrane diameter is 30 mm and the effective area is 4.2 cm2.|
|What cell types can be used with the Millicell ERS?||The MERS will only give meaningful readings on a cell line that is know to form tight junctions e.g. epithelial cells. We strongly recommend using the ERS for those cell lines where published values exist for the expected resistance. Many cell types do not have a large enough resistance to use this technique.|
|What is an ECM and why should it be used?||ECM is extracellular matrix material and is used to coat certain Millicells ( e.g. Millicell CM) to enhance cellular attachment. Normally, attachment dependent cells cannot readily bind to the Biopore membrane so coating the Millicell with collagen or other ECM material will render the filter surface suitable for cell attachment. Please note: the Millicell PCF is tissue culture treated and should not require additional coating. In general, the Millicell HA does not require coating with an ECM and will support direct cell attachment.|
|Why do the electrodes need to be replaced every six months?||In general, electrodes have a 6 month lifetime if they are used continuously. The electrodes have a silver-silver chloride pellete that is depleted everytime it is used. It is the silver silver chloride pellete that creates the potential.|
|What voltage value can I expect for my cell type?||We DO NOT recommend using this function on the instrument. The Millicell-ERS will make voltage readings on only a few kinds of high-voltage generating cells (for example, it will not work on MDCK cells). We do have information on epithelial cells which typically have voltage values of 0-30mV.|
|What resistance value can I expect for my cell types?||Some resistance values have been published for various cell types. Resistance measurements will also depend on the type of cell culture (i.e. cell line, tissue or native cells). In general, the resistance range for epithelial cells is 10-20,000 ohms/cm2. An example of an established epithelial cell line with well defined electrical resistance is MDCK cells. MDCK cells displaying tight junctions show resistance of 5000 ohms/cm2.|
|What is the porosity of the Millicell Culture Plate Inserts?||The porosity of the Millicell Insert membranes are as follows: HATF - approx 80% CM - approx 80% PC and PCF - approx 10%|
|What is the length of the legs on the Millicell Insert unit?||The legs on the Millicell unit are approximately 1-2mm.|
|What is the thickness of the Millicell-PC and -PCF membranes?||The thickness of the PC and PCF membranes are 10 um.|
|What is the thickness of the Millicell-CM membrane?||The thickness of the Millicell-CM memnbrane is approximately 50 um.|
|What is the overall height of the Millicell-CM Organotypic insert?||The overall wall height including the feet is 5 mm.|
|What Volume of media should be used on the inside and outside of the 12 mm and 30mm Millicell cell culture insert devices?||Inside and outside liquid heights are critical and must be adjusted until equal. The suggested volumes below can vary due to plate well volume variations allowed by different cell culture plate manufacturers.
12 mm inserts:
Inside: 0.4 mL
Outside: 0.6 mL
|Which ECM coating do we recommend for endothelial cells?||We recommend the Type 1 Rat Tail Collagen for most endothelial cells. It is recommended to be done with ethanol wetting and is a very cost efficient way to do ECM and seems to be well tolerated by most endothelial cells.|
|What is the difference between Millicell PC and PCF?||Both Millicells have polycarbonate membranes and are frequently used in chemotaxis and transport studies. PC's are recommended ONLY for suspension cell culture. PCF's are tissue culture treated and therefore recommended for attachment dependent cell culture.|
|What is the advantage of using Millicell for cell culture applications ?||Studies have shown that cells actually grow better on microporous membranes than on the artificially smooth surfaces of standard cell culture plasticware. We have found that cells grown on Millicell Cell Culture Plate Inserts produce higher cell densities and greater differentiation than cells grown in standard setups. Cells can be constantly bathed and fed on both their apical and basolateral surfaces and ions and molecules can pass freely across the membrane. Millicells also offer the researcher a more humane approach to animal testing since cell culturing with the units can offer alternatives to live animal studies for many toxicological studies.|
|What size tissue culture plate do I use with the Millicell devices?||The 12mm Millicell insert can be used with a standard 24 well plate, and the 30mm insert is used with a 6 well plate.|
|What is the height of the standard Millicell feet ? ( i.e. how high does the Millicell sit off the floor of the plate?)||The Millicell feet are 1-1.5 mm high.|
|Millicell 24 and Millicell 96|
|Millicell Culture Plate Inserts|
|Millicell Hanging Cell Culture Inserts|
|Millicell-CM Low Height Culture Plate Inserts User Guide|
|Millicell-ERS User Guide|