ABC124 | Anti-phospho-ULK1 (Ser555) Antibody

ABC124
100 µg  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, R, B, Xn WB, PIA Rb Affinity Purified Polyclonal Antibody
      Description
      Catalogue NumberABC124
      DescriptionAnti-phospho-ULK1 (Ser555) Antibody
      Alternate Names
      • Serine/threonine-protein kinase ULK1
      • Autophagy-related protein 1 homolog
      • ATG1
      • hATG1
      • Unc-51-like kinase 1
      Background InformationULK1 or autophagy-related protein 1 homolog (ATG1) is a serine/threonine protein kinase. It is a ubiquitously expressed protein that is highly abundant in the brain, skeletal muscle, and heart. It is involved in the signaling pathway of mTOR receptors leading to the regulation of autophagy in nutrient-deprived cells. mTOR may interact with ULK1 as a complex of ULK1/FIP200/Atg13. ULK1 may play an important role in neuronal development: Elimination of ULK1's enzymatic activity by targeted disruption of its kinase domain has blocked the outgrowth of neurons in the developing mouse brain, possibly by blocking the ULK1-mediated trafficking of neurotrophins.
      References
      Product Information
      FormatAffinity Purified
      Control
      • Non-serum starved and serum starved MCF7 cell lysates
      PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Applications
      ApplicationThis Anti-phospho-ULK1 (Ser555) Antibody is validated for use in Western Blotting, Peptide Inhibition Assay for the detection of phospho-ULK1 (Ser555).
      Key Applications
      • Western Blotting
      • Peptide Inhibition Assay
      Application NotesPeptide Inhibition Analysis: A representative lot of this antibody blocked ULK1 in PIA involving peptide 3123-C vs. peptide 3123 on serum starved MCF7 cell lysates.
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to human ULK1 phosphorylated at Ser555.
      EpitopePhosphorylated Ser555
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityThis antibody recognizes ULK1 phosphorylated at Ser555.
      Species Reactivity
      • Human
      • Mouse
      • Rat
      • Bovine
      • Xenopus
      Species Reactivity NoteDemonstrated to react with Human. Predicted to react with Mouse, Rat, Bovine, and Xenopus based on 100% sequence homology.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • ULK1
      Modifications
      • Phosphorylation
      Purification MethodAffinity Purfied
      UniProt Number
      UniProt SummaryFUNCTION: Serine/threonine-protein kinase involved in autophagy in response to starvation. Acts upstream of phosphatidylinositol 3-kinase PIK3C3 to regulate the formation of autophagophores, the precursors of autophagosomes. Part of regulatory feedback loops in autophagy: acts both as a downstream effector and negative regulator of mammalian target of rapamycin complex 1 (mTORC1) via interaction with RPTOR. Activated via phosphorylation by AMPK and also acts as a regulator of AMPK by mediating phosphorylation of AMPK subunits PRKAA1, PRKAB2 and PRKAG1, leading to negatively regulate AMPK activity. May phosphorylate ATG13/KIAA0652 and RPTOR; however such data need additional evidences. Plays a role early in neuronal differentiation and is required for granule cell axon formation.

      CATALYTIC ACTIVITY: ATP + a protein = ADP + a phosphoprotein.

      SUBUNIT STRUCTURE: ATP + a protein = ADP + a phosphoprotein.

      SUBCELLULAR LOCATION: Cytoplasm > cytosol. Preautophagosomal structure. Note: Under starvation conditions, is localized to puncate structures primarily representing the isolation membrane that sequesters a portion of the cytoplasm resulting in the formation of an autophagosome.

      TISSUE SPECIFICITY: Ubiquitously expressed. Detected in the following adult tissues: skeletal muscle, heart, pancreas, brain, placenta, liver, kidney, and lung.

      POST-TRANSLATIONAL MODIFICATION: Autophosphorylated. Phosphorylated under nutrient-rich conditions; dephosphorylated during starvation or following treatment with rapamycin. Under nutrient sufficiency, phosphorylated by MTOR/mTOR, disrupting the interaction with AMPK and preventing activation of ULK1. In response to nutrient limitation, phosphorylated and activated by AMPK, leading to activate autophagy.

      SEQUENCE SIMILARITIES: Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. APG1/unc-51/ULK1 subfamily. Contains 1 protein kinase domain.

      SEQUENCE CAUTION: The sequence BAA34442.2 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.
      Molecular Weight~120 kDa observed. Uniprot describes a molecular weight at 112 kDa. This protein has been observed at greater than ~125 kDa due to multiple phosphorylation-dephosphorylation processes. (Ganley, I. G., et al. (2009). J Biol Chem. 284(18):12297-12305.)
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in non-serum starved and serum starved MCF7 cell lysates.

      Western Blot Analysis: 0.2 µg/mL of this antibody detected phospho-ULK1 (Ser555) in 10 µg of non-serum starved and serum starved MCF7 cell lysates.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-phospho-ULK1 (Ser555) - 2449230 2449230
      Anti-phospho-ULK1 (Ser555) - 2041599 2041599
      Anti-phospho-ULK1 (Ser555) - Q1985262 Q1985262
      Anti-phospho-ULK1 (Ser555) -2505102 2505102
      Anti-phospho-ULK1 (Ser555) -2647556 2647556
      Anti-phospho-ULK1 (Ser555) -2791202 2791202
      Anti-phospho-ULK1 (Ser555) -2892519 2892519

      References

      Reference overviewPub Med ID
      PHY906(KD018), an adjuvant based on a 1800-year-old Chinese medicine, enhanced the anti-tumor activity of Sorafenib by changing the tumor microenvironment.
      Lam, W; Jiang, Z; Guan, F; Huang, X; Hu, R; Wang, J; Bussom, S; Liu, SH; Zhao, H; Yen, Y; Cheng, YC
      Scientific reports  5  9384  2015

      Show Abstract
      25819872 25819872

      Brochure

      Title
      Autophagy Solutions - Novel antibodies, inhibitors and innovative assays

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies