AB10615 | Anti-PDEF Antibody

AB10615
100 µg  
Retrieving price...
Price could not be retrieved
Minimum Quantity needs to be mulitiple of
Upon Order Completion More Information
You Saved ()
 
Request Pricing
Limited AvailabilityLimited Availability
Stocked 
Discontinued
Limited Quantities Available
Available
    Remaining : Will advise
      Remaining : Will advise
      Will advise
      Contact Customer Service

      Special Offers

       

      Contact Customer Service

      Click To Print This Page

      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H ELISA, WB Rb Affinity Purified Polyclonal Antibody
      Description
      Catalogue NumberAB10615
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-PDEF Antibody
      Alternate Names
      • Prostate-derived Ets Factor
      Background InformationPDEF (prostate-derived Ets factor) is a 37.5-kDa protein that acts as an androgen-independent transcriptional activator for the PSA (prostate specific antigen) promoter. It also interacts directly with the DNA binding domain of androgen receptor and enhances androgen-mediated activation of the PSA promoter. PDEF was shown to have transcript levels 192-fold higher in the peripheral blood of some breast cancer patients in comparison with normal individuals. PDEF mRNA is also frequently over-expressed in human breast tumors as well as in human ovarian tumors. In ovarian tumors, PDEF expression seems to down-modulate malignant potential, and thus provides a rationale to screen for drugs that induce PDEF expression in epithelial ovarian tumors.
      References
      Product Information
      FormatAffinity Purified
      PresentationThis polyclonal antibody is supplied in 400 μL of phosphate buffered saline (pH 7.4) containing 0.1% sodium azide.
      Applications
      ApplicationDetect PDEF using this Anti-PDEF Antibody validated for use in ELISA & WB.
      Key Applications
      • ELISA
      • Western Blotting
      Application NotesELISA: 0.1-1μg/mL
      Western Blotting: 1-3 μg/mL
      Biological Information
      ImmunogenSynthetic peptide derived from the internal region of human PDEF (prostate-derived Ets factor).
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityRecognizes PDEF.
      Species Reactivity
      • Human
      Species Reactivity NoteHuman (positive control: LNCaP cells)
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryPDEF is an ETS transcription factor expressed in prostate epithelial cells. It acts as an androgen-independent transactivator of PSA (MIM 176820) expression.[supplied by OMIM]
      Gene Symbol
      • SPDEF
      • bA375E1.3
      • PSE
      • PDEF
      • RP11-375E1__A.3
      Purification MethodImmunoAffinity Purified
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: O95238 # May function as an androgen-independent transactivator of the prostate-specific antigen (PSA) promoter. Binds to 5'-GGAT- 3' DNA sequences. May play a role in the regulation of the prostate gland and/or prostate cancer development. Acts as a transcriptional activator for SERPINB5 promoter.
      SIZE: 335 amino acids; 37518 Da
      SUBUNIT: Interacts with the DNA-binding domain of the androgen receptor. Interacts with NKX3-1.
      SUBCELLULAR LOCATION: Nucleus (Probable).
      TISSUE SPECIFICITY: Expressed in a very restricted set of primarily hormone-regulated epithelial tissues with particularly high expression in the prostate gland. Significantly lower expression is seen in other hormone regulated tissues such as mammary gland, salivary gland, and ovary. Expressed in prostate carcinoma cells.
      SIMILARITY: SwissProt: O95238 ## Belongs to the ETS family. & Contains 1 ETS DNA-binding domain. & Contains 1 PNT (pointed) domain.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStore at -20°C in undiluted aliquots for 1 year from date of shipment.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      RABBIT ANTI-PDEF POLYCLONAL ANTIBODY - 2002176 2002176

      References

      Reference overviewPub Med ID
      Prostate epithelium-derived Ets transcription factor mRNA is overexpressed in human breast tumors and is a candidate breast tumor marker and a breast tumor antigen.
      Ghadersohi, A and Sood, A K
      Clin. Cancer Res., 7: 2731-8 (2001)  2001

      Show Abstract
      11555586 11555586
      PDEF, a novel prostate epithelium-specific ets transcription factor, interacts with the androgen receptor and activates prostate-specific antigen gene expression.
      Oettgen, P, et al.
      J. Biol. Chem., 275: 1216-25 (2000)  2000

      Show Abstract
      10625666 10625666

      Data Sheet

      Title
      Anti-PDEF - Data Sheet