07-789 | Anti-phospho-AKT (Thr34) Antibody

100 µL  
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      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H WB Rb Affinity Purified Polyclonal Antibody
      Catalogue Number07-789
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-phospho-AKT (Thr34) Antibody
      Alternate Names
      • Akt1
      • PKB
      • PRKBA
      • RAC
      • RAC-ALPHA
      Product Information
      FormatAffinity Purified
      Presentation100 μL of epitope specific affinity purified rabbit polyclonal IgG in 50% affinity storage buffer (PBS containing 1.0 mg/mL BSA and 0.05% sodium azide) and 50% glycerol.
      ApplicationAnti-phospho-AKT (Thr34) Antibody detects level of phospho-AKT (Thr34) & has been published & validated for use in WB.
      Key Applications
      • Western Blotting
      Biological Information
      ImmunogenKLH conjugated peptide corresponding to amino acid region encompassing the human phospho-AKT (Thr34); Sequence highly conserved in mouse and rat.
      SpecificityRecognizes phospho-AKT (Thr34)
      Species Reactivity
      • Human
      Species Reactivity NotePredicted to cross react with Mouse and Rat due to high sequence homology, but have not been tested.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThe serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Multiple alternatively spliced transcript variants have been found for this gene.
      Gene Symbol
      • AKT1
      • RAC
      • PRKBA
      • MGC99656
      • RAC-ALPHA
      • RAC-PK-alpha
      • C-AKT
      • PKB
      • AKT
      • Phosphorylation
      Purification MethodImmunoAffinity Purified
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P31749 # General protein kinase capable of phosphorylating several known proteins. Phosphorylates TBC1D4. Signals downstream of phosphatidylinositol 3-kinase (PI(3)K) to mediate the effects of various growth factors such as platelet-derived growth factor (PDGF), epidermal growth factor (EGF), insulin and insulin-like growth factor I (IGF-I). Plays a role in glucose transport by mediating insulin-induced translocation of the GLUT4 glucose transporter to the cell surface. Mediates the antiapoptotic effects of IGF-I. Mediates insulin-stimulated protein synthesis, partly by playing a role in both insulin-induced phosphorylation of 4E-BP1 and in insulin-induced activation of p70 S6 kinase. Promotes glycogen synthesis by mediating the insulin-induced activation of glycogen synthase.
      SIZE: 480 amino acids; 55686 Da
      SUBUNIT: Interacts with CENTG1 isoform 2 (PIKE-A) in the presence of guanine nucleotides. The C-terminus interacts with CCDC88A/GRDN. Interacts with AKTIP.
      SUBCELLULAR LOCATION: Cytoplasm. Nucleus. Note=Nucleus after activation by integrin-linked protein kinase 1 (ILK1).
      TISSUE SPECIFICITY: In all human cell types so far analyzed.
      DOMAIN: SwissProt: P31749 Binding of the PH domain to the phosphatidylinositol 3- kinase alpha (PI(3)K) results in its targeting to the plasma membrane.
      PTM: Phosphorylation on Thr-308, Ser-473 and Tyr-474 is required for full activity. Ser-473 phosphorylation by the Rictor-mTor complex favors Thr-308 phosphorylation by PDPK1. Ser-473 phosphorylation is enhanced by interaction with CENTG1 isoform 2 (PIKE-A).
      SIMILARITY: Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. RAC subfamily. & Contains 1 AGC-kinase C-terminal domain. & Contains 1 PH domain. & Contains 1 protein kinase domain.
      Molecular Weight60 kDa
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by immunoblot.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 2 years at -20°C from date of shipment. Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol-containing solutions to become frozen during storage.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information




      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-phospho-AKT (Thr34) (Affinity purified Rabbit polyclonal IgG) - 2442549 2442549
      Anti-phospho-AKT (Thr34) (Affinity purified Rabbit polyclonal IgG) - DAM1467540 DAM1467540
      Anti-phospho-AKT (Thr34) - 0603026241 0603026241


      Reference overviewApplicationPub Med ID
      Progesterone receptor enhances breast cancer cell motility and invasion via extranuclear activation of focal adhesion kinase.
      Fu, XD; Goglia, L; Sanchez, AM; Flamini, M; Giretti, MS; Tosi, V; Genazzani, AR; Simoncini, T
      Endocrine-related cancer  17  431-43  2010

      Show Abstract
      Western Blotting20233709 20233709
      Extra-nuclear signaling of progesterone receptor to breast cancer cell movement and invasion through the actin cytoskeleton.
      Fu, XD; Giretti, MS; Baldacci, C; Garibaldi, S; Flamini, M; Sanchez, AM; Gadducci, A; Genazzani, AR; Simoncini, T
      PloS one  3  e2790  2008

      Show Abstract Full Text Article
      Immunoblotting (Western)18665217 18665217
      Cisplatin activates Akt in small cell lung cancer cells and attenuates apoptosis by survivin upregulation
      Belyanskaya, Larisa L, et al
      Int J Cancer, 117:755-63 (2005)  2005

      15981204 15981204
      Ceramide disables 3-phosphoinositide binding to the pleckstrin homology domain of protein kinase B (PKB)/Akt by a PKCzeta-dependent mechanism
      Powell, Darren J, et al
      Mol Cell Biol, 23:7794-808 (2003)  2003

      14560023 14560023
      PKB/Akt: connecting phosphoinositide 3-kinase to cell survival and beyond.
      Marte, B M and Downward, J
      Trends Biochem. Sci., 22: 355-8 (1997)  1997

      Show Abstract
      9301337 9301337

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      Life Science Research > Antibodies and Assays > Primary Antibodies