07-175 | Anti-phospho-JNK (Thr183/Tyr185, Thr221/Tyr223) Antibody

Immunoblot Analysis: 10 μg of untreated (lane 1) or anisomycin treated (30 min) (lane 2) NIH/3T3 cell lysates were resolved by electrophoresis, transferred to Immobilon-P PVDF membrane, and probed with anti-phospho-JNK 1/2 (Thr183/Tyr185, Thr221/Tyr223) for 2 hours at room temperature. Proteins were visualized via HRP and chemiluminescent detection. Arrow indicates phospho-JNK 1 &2.
Immunoblot Analysis: 10 μg of untreated (lane 1) or anisomycin treated (30 min) (lane 2) NIH/
07-175
200 µg  
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      Overview

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      Eq, M, H, R, Ca, Xn, Ch, BIP, WB, EARbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue Number 07-175
      Synonyms protein kinase JNK1 JNK1 alpha protein kinase JNK1 beta protein kinase JUN N-terminal kinase Stress-activated protein kinase JNK1 mitogen-activated protein kinase 8 mitogen-activated protein kinase 8 isoform JNK1 alpha1
      Description Anti-phospho-JNK (Thr183/Tyr185, Thr221/Tyr223) Antibody
      Background Information JNK1(MAPK8) is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is activated by various cell stimuli, and targets specific transcription factors, and thus mediates immediate-early gene expression in response to cell stimuli. The activation of this kinase by tumor-necrosis factor alpha (TNF-α) is found to be required for TNF-alpha induced apoptosis. This kinase is also involved in UV radiation induced apoptosis, which is thought to be related to cytochrome c-mediated cell death pathway. Studies of the mouse counterpart of this gene suggested that this kinase play a key role in T cell proliferation, apoptosis and differentiation. Four alternatively spliced transcript variants encoding distinct isoforms have been reported. JNK1 is activated by threonine and tyrosine phosphorylation by either of two dual specificity kinases, MAP2K4 and MAP2K7. The JNK pathway is critically involved in diabetes and levels are abnormally elevated in obesity. The cell-permeable JNK inhibitory peptide may have promise as a therapeutic agent for diabetes.
      References
      Product Information
      Format Affinity Purified
      Specificity This antibody recognizes JNK when phosphorylated at Thr183/Tyr185 and Thr221/Tyr223 .
      Control
      • Untreated and Lambda phosphatase treated RAW264.7 cell lysates
      Presentation Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.
      Applications
      Application Detect phospho-JNK (Thr183/Tyr185 using this Anti-phospho-JNK (Thr183/Tyr185, Thr221/Tyr223) Antibody validated for use in WB, EA & IP.
      Key Applications
      • Immunoprecipitation
      • Western Blotting
      • Enzyme Assay
      Application Notes Immunoprecipitation Analysis: 10 µg from a previous lot immunoprecipitated JNK phosphorylated at Thr183/Tyr185 and Thr221/Tyr223 from RAW264.7 cell lysate.
      Biological Information
      Immunogen species KLH-conjugated linear peptide corresponding to human JNK phosphorylated at Thr183/Tyr185 and Thr221/Tyr223.
      Epitope Phosphorylated at Thr183/Tyr185 and Thr221/Tyr223
      Concentration Please refer to the Certificate of Analysis for the lot-specific concentration.
      Host Rabbit
      Specificity This antibody recognizes JNK when phosphorylated at Thr183/Tyr185 and Thr221/Tyr223 .
      Isotype IgG
      Species Reactivity Horse Mouse Human Rat Canine Xenopus Chicken Bovine
      Species Reactivity Note Demonstrated to react with mouse and human. Predicted to react with rat, canine, xenopus, chicken, bovine, and equine based on 100% sequence homology.
      Antibody Type Polyclonal Antibody
      Entrez Gene Number
      Entrez Gene Summary The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is activated by various cell stimuli, and targets specific transcription factors, and thus mediates immediate-early gene expression in response to cell stimuli. The activation of this kinase by tumor-necrosis factor alpha (TNF-alpha) is found to be required for TNF-alpha induced apoptosis. This kinase is also involved in UV radiation induced apoptosis, which is thought to be related to cytochrom c-mediated cell death pathway. Studies of the mouse counterpart of this gene suggested that this kinase play a key role in T cell proliferation, apoptosis and differentiation. Four alternatively spliced transcript variants encoding distinct isoforms have been reported.
      Gene Symbol
      • JNK1A2
      • MAPK8
      • JNK1
      • JNK21B1/2
      • PRKM8
      • JNK-46
      • JNK
      • SAPK1
      Modifications
      • Phosphorylation
      Purification Method Affinity purified
      UniProt Number
      UniProt Summary FUNCTION: JNK1 isoforms display different binding patterns: beta-1 preferentially binds to c-Jun, whereas alpha-1, alpha-2, and beta- 2 have a similar low level of binding to both c-Jun or ATF2. However, there is no correlation between binding and phosphorylation, which is achieved at about the same efficiency by all isoforms.

      COFACTOR: Magnesium.

      SIZE: 427 amino acids; 48296 Da

      SUBUNIT: Binds to at least four scaffolding proteins, MAPK8IP1/JIP-1, MAPK8IP2/JIP-2, MAPK8IP3/JIP-3/JSAP1 and SPAG9/MAPK8IP4/JIP-4. These proteins also bind other components of the JNK signaling pathway. Interacts with TP53 and WWOX. Interacts with JAMP (By similarity).

      DOMAIN: The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.

      PTM: Dually phosphorylated on Thr-183 and Tyr-185, which activates the enzyme.

      SIMILARITY: Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily. & Contains 1 protein kinase domain. ... hide » see more »
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assurance Evaluated by Western Blot in untreated and lambda phosphatase treated RAW264.7 cell lysates.

      Western Blot Analysis: 2 µg/mL of this antibody detected JNK on 10 µg of untreated and lambda phosphatase RAW264.7 cell lysates.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions Stable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size 200 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      MSDS

      Languages
      US English

      Certificates of Analysis

      TitleLot Number
      Anti-phospho-JNK (Thr183/Tyr185, Thr221/Tyr223) - 2430491 2430491
      Anti-phospho-JNK (Thr183/Tyr185, Thr221/Tyr223) - DAM1713218 DAM1713218
      Anti-phospho-JNK (Thr183/Tyr185, Thr221/Tyr223) - DAM1810820 DAM1810820
      Anti-phospho-JNK (Thr183/Tyr185, Thr221/Tyr223) - 20316 20316
      Anti-phospho-JNK (Thr183/Tyr185, Thr221/Tyr223) - 2073119 2073119
      Anti-phospho-JNK (Thr183/Tyr185, Thr221/Tyr223) - 2276339 2276339
      Anti-phospho-JNK (Thr183/Tyr185, Thr221/Tyr223) - 2318094 2318094
      Anti-phospho-JNK (Thr183/Tyr185, Thr221/Tyr223) - 2517878 2517878
      Anti-phospho-JNK (Thr183/Tyr185, Thr221/Tyr223) - 30813 30813
      Anti-phospho-JNK (Thr183/Tyr185, Thr221/Tyr223) - 32347 32347
      Anti-phospho-JNK (Thr183/Tyr185, Thr221/Tyr223) - DAM1394789 DAM1394789
      Anti-phospho-JNK (Thr183/Tyr185, Thr221/Tyr223) - DAM1661070 DAM1661070
      Anti-phospho-JNK (Thr183/Tyr185, Thr221/Tyr223) - DAM1770310 DAM1770310
      Anti-phospho-JNK (Thr183/Tyr185, Thr221/Tyr223) - JBC1361845 JBC1361845

      References

      Reference overviewPub Med ID
      Activation of c-Jun N-terminal kinase (JNK) during mitosis in retinal progenitor cells.
      Vinicius Toledo Ribas,Bruno Souza Gonçalves,Rafael Linden,Luciana Barreto Chiarini
      PloS one 7 2012

      Show Abstract
      22496813
      CD22 regulates adaptive and innate immune responses of B cells.
      Norihito Kawasaki,Christoph Rademacher,James C Paulson
      Journal of innate immunity 3 2011

      Show Abstract
      21178327
      Decoy receptor 3, upregulated by Epstein-Barr virus latent membrane protein 1, enhances nasopharyngeal carcinoma cell migration and invasion.
      Cheng-Hsun Ho, Chi-Long Chen, Wing-Yin Li, Chi-Ju Chen, Cheng-Hsun Ho, Chi-Long Chen, Wing-Yin Li, Chi-Ju Chen
      Carcinogenesis 30 1443-51 2009

      Show Abstract
      19483191
      Subcellular receptor redistribution and enhanced microspike formation by a Ret receptor preferentially recruiting Dok.
      Anna Stenqvist, T Kalle Lundgren, Matthew J Smith, Ola Hermanson, Gonçalo Castelo-Branco, Tony Pawson, Patrik Ernfors
      Neuroscience letters 435 11-6 2008

      Show Abstract
      18353552
      WNT pathways in the neonatal ovine uterus: potential specification of endometrial gland morphogenesis by SFRP2.
      Kanako Hayashi, Thomas E Spencer
      Biology of reproduction 74 721-33 2006

      Show Abstract
      16407498