07-122 | Anti-Kalirin Antibody

07-122
200 µg  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      M, R IP, WB, ICC Rb Purified Polyclonal Antibody
      Description
      Catalogue Number07-122
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-Kalirin Antibody
      References
      Product Information
      FormatPurified
      Presentation0.1M Tris-glycine, pH 7.4, 0.15M NaCl, 0.05% sodium azide prior to the addition of glycerol to 30%
      Applications
      ApplicationAnti-Kalirin Antibody is an antibody against Kalirin for use in IP, WB & IC.
      Key Applications
      • Immunoprecipitation
      • Western Blotting
      • Immunocytochemistry
      Biological Information
      ImmunogenGST fusion protein corresponding to residues 517-976 of rat Kalirin, cleaved with thrombin to remove the GST moiety; referred to as JH2582
      HostRabbit
      SpecificityKalirin; does not cross react with Trio
      IsotypeIgG
      Species Reactivity
      • Mouse
      • Rat
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryHuntington's disease (HD), a neurodegenerative disorder characterized by loss of striatal neurons, is caused by an expansion of a polyglutamine tract in the HD protein huntingtin. This gene encodes a protein that interacts with the huntingtin-associated protein 1, which is a huntingtin binding protein that may function in vesicle trafficking. Alternatively spliced transcript variants encoding different isoforms have been described.
      Gene Symbol
      • KALRN
      • TRAD
      • Hs.8004
      • DUET
      • duo
      • Kalirin
      • FLJ16443
      • DUO
      • HAPIP
      Purification MethodProtein A chromatography
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: O60229 # Promotes the exchange of GDP by GTP. Activates specific Rho GTPase family members, thereby inducing various signaling mechanisms that regulate neuronal shape, growth, and plasticity, through their effects on the actin cytoskeleton. Induces lamellipodia independent of its GEF activity.
      COFACTOR: Magnesium.
      SIZE: 2985 amino acids; 340174 Da
      SUBUNIT: Interacts with the C-terminal of peptidylglycine alpha- amidating monooxygenase (PAM) and with the huntingtin-associated protein 1 (HAP1) (By similarity).
      SUBCELLULAR LOCATION: Cytoplasm. Cytoplasm, cytoskeleton. Note=Associated with the cytoskeleton.
      TISSUE SPECIFICITY: Isoform 2 is brain specific. Highly expressed in cerebral cortex, putamen, amygdala, hippocampus and caudate nucleus. Weakly expressed in brain stem and cerebellum. Isoform 4 is expressed in skeletal muscle.
      DOMAIN: SwissProt: O60229 The two GEF domains catalyze nucleotide exchange for RAC1 and RhoA which are bound by DH1 and DH2 respectively. The two GEF domains appear to play differing roles in neuronal development and axonal outgrowth. SH3 1 binds to the first GEF domain inhibiting GEF activity only when in the presence of a PXXP peptide, suggesting that the SH3 domain/peptide interaction mediates binding to GEF1. CRK1 SH3 domain binds to and inhibits GEF1 activity (By similarity).
      PTM: Autophosphorylated.
      DISEASE: SwissProt: O60229 # Genetic variation in KALRN is associated with susceptibility to coronary heart disease type 5 (CHDS5) [MIM:608901]. CHD is the leading cause of death and disability worldwide. CHD is multifactorial disease with a strong genetic component. Classic epidemiologic studies have revealed many risk factors for CHD, including age, sex, hypertension, dyslipidemia, diabetes mellitus, smoking, and physical inactivity.
      SIMILARITY: Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. & Contains 1 CRAL-TRIO domain. & Contains 2 DH (DBL-homology) domains. & Contains 1 fibronectin type-III domain. & Contains 1 Ig-like C2-type (immunoglobulin-like) domain. & Contains 2 PH domains. & Contains 1 protein kinase domain. & Contains 1 RPR domain. & Contains 2 SH3 domains. & Contains 5 spectrin repeats.
      MISCELLANEOUS: Called DUO because the encoded protein is closely related to but shorter than TRIO.
      Molecular Weight190, 115, 370 and 420kDa isoforms
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assuranceroutinely evaluated by immunoblot on RIPA lysates from rat brain microsomal protein preparation; long exposure times may be required
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions2 years at -20°C
      Packaging Information
      Material Size200 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Certificates of Analysis

      TitleLot Number
      07-122 - 2280413 2280413
      Anti-Kalirin (rabbit polyclonal IgG) 2905318
      Anti-Kalirin (rabbit polyclonal IgG) 2880746
      Anti-Kalirin (rabbit polyclonal IgG) - 2015760 2015760
      Anti-Kalirin (rabbit polyclonal IgG) - 2032852 2032852
      Anti-Kalirin (rabbit polyclonal IgG) - 2155411 2155411
      Anti-Kalirin (rabbit polyclonal IgG) - 2332542 2332542
      Anti-Kalirin - 20095 20095
      Anti-Kalirin - 2043526 2043526
      Anti-Kalirin - DAM1576930 DAM1576930

      References

      Reference overviewApplicationSpeciesPub Med ID
      The Rho-GEF Kalirin regulates bone mass and the function of osteoblasts and osteoclasts.
      Huang, S; Eleniste, PP; Wayakanon, K; Mandela, P; Eipper, BA; Mains, RE; Allen, MR; Bruzzaniti, A
      Bone  60  235-45  2014

      Show Abstract
      Western Blotting24380811 24380811
      In vivo quantitative proteomics of somatosensory cortical synapses shows which protein levels are modulated by sensory deprivation.
      Butko, MT; Savas, JN; Friedman, B; Delahunty, C; Ebner, F; Yates, JR; Tsien, RY
      Proceedings of the National Academy of Sciences of the United States of America  110  E726-35  2013

      Show Abstract
      ImmunohistochemistryMouse23382246 23382246
      Postsynaptic density scaffold SAP102 regulates cortical synapse development through EphB and PAK signaling pathway.
      Murata, Y; Constantine-Paton, M
      The Journal of neuroscience : the official journal of the Society for Neuroscience  33  5040-52  2013

      Show Abstract
      Western Blotting23486974 23486974
      Kalirin-7 mediates cocaine-induced AMPA receptor and spine plasticity, enabling incentive sensitization.
      Wang, X; Cahill, ME; Werner, CT; Christoffel, DJ; Golden, SA; Xie, Z; Loweth, JA; Marinelli, M; Russo, SJ; Penzes, P; Wolf, ME
      The Journal of neuroscience : the official journal of the Society for Neuroscience  33  11012-22  2013

      Show Abstract
      23825406 23825406
      RasGRF2 Rac-GEF activity couples NMDA receptor calcium flux to enhanced synaptic transmission.
      Schwechter, B; Rosenmund, C; Tolias, KF
      Proceedings of the National Academy of Sciences of the United States of America  110  14462-7  2013

      Show Abstract
      23940355 23940355
      Increased expression of Kalirin-9 in the auditory cortex of schizophrenia subjects: its role in dendritic pathology.
      Deo, AJ; Cahill, ME; Li, S; Goldszer, I; Henteleff, R; Vanleeuwen, JE; Rafalovich, I; Gao, R; Stachowski, EK; Sampson, AR; Lewis, DA; Penzes, P; Sweet, RA
      Neurobiology of disease  45  796-803  2012

      Show Abstract
      22120753 22120753
      Social, Communication, and Cortical Structural Impairments in Epac2-Deficient Mice.
      Srivastava, Deepak P, et al.
      J. Neurosci., 32: 11864-11878 (2012)  2012

      Show Abstract
      22915127 22915127
      β-Amyloid 42/40 ratio and kalirin expression in Alzheimer disease with psychosis.
      Patrick S Murray,Caitlin M Kirkwood,Megan C Gray,Milos D Ikonomovic,William R Paljug,Eric E Abrahamson,Ruth A Henteleff,Ronald L Hamilton,Julia K Kofler,William E Klunk,Oscar L Lopez,Peter Penzes,Robert A Sweet
      Neurobiology of aging  33  2012

      Show Abstract
      22429885 22429885
      A study of the spatial protein organization of the postsynaptic density isolated from porcine cerebral cortex and cerebellum.
      Yun-Hong, Y; Chih-Fan, C; Chia-Wei, C; Yen-Chung, C
      Molecular & cellular proteomics : MCP  10  M110.007138  2011

      Show Abstract
      21715321 21715321
      An isoform of kalirin, a brain-specific GDP/GTP exchange factor, is enriched in the postsynaptic density fraction.
      Penzes, P, et al.
      J. Biol. Chem., 275: 6395-403 (2000)  2000

      Show Abstract
      10692441 10692441