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407321 | γ-Interferon, Rat, Recombinant, E. coli

407321
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      Overview

      Replacement Information

      Pricing & Availability

      Catalog NumberAvailability Packaging Qty/Pack Price Quantity
      407321-100UG
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      Stocked 
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      Available
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          Glass bottle 100 μg
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          Description
          OverviewRecombinant rat interferon-γ (IFN-γ) expressed in E. coli as a single, non-glycosylated polypeptide containing 135 amino acids. Purified by chromatographic methods. IFN-γ is a type II cytokine produced by activated T cells and natural killer cells. It plays a significant role immunoregulation and has been implicated in the induction of the expression of class II histocompatibility molecules in cells that do not normally express them, resulting in autoimmune disease.
          Catalogue Number407321
          Brand Family Calbiochem®
          References
          ReferencesAlvarado, J., et al. 2005. Immunol. Lett. 99, 109.
          Di Rosa, M., et al. 2005. Clin. Chem. Lab. Med. 43, 499.
          Malaguarnera, L., et al. 2005. J. Clin. Lab. Anal. 19, 128.
          Product Information
          FormLyophilized
          FormulationLyophilized from 0.5X PBS, pH 7.2.
          Applications
          Biological Information
          Biological activityReported as U/mg on a lot-specific basis using a cytopathic affect inhibition assay with NIH/3T3 cells challenged with EMC virus.
          Purity≥95% by RP-HPLC
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Blue Ice Only
          Toxicity Standard Handling
          Storage -20°C
          Protect from Moisture Protect from moisture
          Do not freeze Ok to freeze
          Special InstructionsFollowing reconstitution, refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C) for long-term storage. Stock solutions are stable for up to 7 days at 4°C or for up to 4 months at -20°C. Avoid freeze/thaw cycles of solutions.
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          SDS

          Title

          Safety Data Sheet (SDS) 

          Certificates of Analysis

          TitleLot Number
          407321

          References

          Reference overview
          Alvarado, J., et al. 2005. Immunol. Lett. 99, 109.
          Di Rosa, M., et al. 2005. Clin. Chem. Lab. Med. 43, 499.
          Malaguarnera, L., et al. 2005. J. Clin. Lab. Anal. 19, 128.
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision22-July-2010 JSW
          DescriptionRecombinant rat interferon-γ (IFN-γ) expressed in E. coli as a single, non-glycosylated polypeptide containing 135 amino acids. Purified by chromatographic methods. IFN-γ is a type II cytokine produced by activated T cells and natural killer cells. It plays a significant role immunoregulation and has been implicated in the induction of the expression of class II histocompatibility molecules in cells that do not normally express them, resulting in autoimmune disease.
          FormLyophilized
          FormulationLyophilized from 0.5X PBS, pH 7.2.
          Purity≥95% by RP-HPLC
          Biological activityReported as U/mg on a lot-specific basis using a cytopathic affect inhibition assay with NIH/3T3 cells challenged with EMC virus.
          SolubilityReconstitute with sterile water to ≥100 µg/ml, containing 0.1% HSA or BSA. Further dilute with aqueous buffers just prior to use.
          Storage -20°C
          Protect from moisture
          Do Not Freeze Ok to freeze
          Special InstructionsFollowing reconstitution, refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C) for long-term storage. Stock solutions are stable for up to 7 days at 4°C or for up to 4 months at -20°C. Avoid freeze/thaw cycles of solutions.
          Toxicity Standard Handling
          ReferencesAlvarado, J., et al. 2005. Immunol. Lett. 99, 109.
          Di Rosa, M., et al. 2005. Clin. Chem. Lab. Med. 43, 499.
          Malaguarnera, L., et al. 2005. J. Clin. Lab. Anal. 19, 128.