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ECM530 | α Integrin-mediated Cell Adhesion Array Kit, colorimetric

ECM530
1 plate  96 wells
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      Overview

      Replacement Information

      Key Specifications Table

      Detection Methods
      Chromogenic
      Description
      Catalogue NumberECM530
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      Descriptionα Integrin-mediated Cell Adhesion Array Kit, colorimetric
      OverviewIntroduction

      Cell adhesion plays a major role in cellular communication and regulation, and is of fundamental importance in the development and maintenance of tissues. A family of cell surface receptors, the integrins, mediates cell attachment to proteins of the extracellular matrix. Integrins are heterodimer molecules containing an alpha and beta transmembrane glycoprotein subunit that are non-covalently bound together (1-2). Historically, antibodies have been used to determine the integrin profiles of cells by immunoprecipitation, immunofluorescence, immunoblotting, and flow cytometry. These methods are time-consuming, laborious or require sophisticated equipment.

      The CHEMICON® Alpha Integrin-Mediated Cell Adhesion Array kit is designed as a cost effective, efficient method for identification of cell surface integrins. The kit format uses 12 x 8-well removable strips in a plate frame for convenience and flexibility in designing assays. The Integrin-Mediated Cell Adhesion Array can be completed within 1-2 hours, providing a rapid, quantitative assay for the characterization of alpha integrin surface expression.

      In addition, Chemicon continues to provide numerous migration, invasion, and adhesion products including:

      · Beta Integrin-Mediated Cell Adhesion Array (ECM531)

      · Alpha/Beta Integrin-Mediated Cell Adhesion Array Combo Kit (ECM532)

      · QCM™ 8μm 96-well Chemotaxis Cell Migration Assay (ECM510)

      · QCM™ 5μm 96-well Chemotaxis Cell Migration Assay (ECM512)

      · QCM™ 3μm 96-well Chemotaxis Cell Migration Assay (ECM515)

      · QCM™ 96-well Cell Invasion Assay (ECM555)

      · 24-well Insert Cell Migration and Invasion Assay Systems

      · CytoMatrix™ Cell Adhesion strips (ECM protein coated)

      · QuantiMatrix™ ECM protein ELISA kits



      Test Principle

      The CHEMICON® Alpha Integrin-Mediated Cell Adhesion Array uses mouse monoclonal antibodies generated against human alpha integrins/subunits (alpha1, alpha2, alpha3, alpha4, alpha5, alphaV, and alphavbeta3), that are immobilized onto a goat anti-mouse antibody coated microtiter plate. The plate is then used to capture cells expressing these integrins on their cell surface; goat anti-mouse antibody coated wells are provided as a negative assay control. Each strip (8 wells total) consists of 7 integrin antibody coated wells and one negative control well (see plate layout on page 3). Experimental cells are seeded onto the coated substrate and incubated. Subsequently, unbound cells are washed away, and adherent cells are fixed and stained. Relative cell attachment is determined using absorbance readings.

      Additional information about the monoclonals provided in the kit:

      * Mouse anti-Alpha 1 [CD49a], clone FB12, (Hu)

      * Mouse anti-Alpha 2 [CD49b], clone P1E6, (Hu)

      * Mouse anti-Alpha 3 [CD49c], clone P1B5, (Hu)

      * Mouse anti-Alpha 4 [CD49d], cloneP1H4. (Hu, Prim)

      * Mouse anti-Alpha 5 [CD49e], clone P1D6, (Hu)

      * Mouse anti-Alpha V [CD51], clone P3G8, (Hu)

      * Mouse anti-Alpha V/Beta 3 [CD51/CD61], clone LM609, (Hu, Bov, Por, Av, Can, Chk, Mky, Rb)
      Materials Required but Not Delivered· Multi-channel or repeating pipettes

      · Microplate reader (540-570 nm detection) or spectrophotometer

      · CO2 incubator appropriate for subject cells

      · Hemacytometer and microscope for counting cells

      · Dissociation Buffer

      · Deionized water

      · PBS
      References
      Product Information
      Components
      • Alpha Integrin Array Plate: (PN: 90599) One 96-well plate with 12 strips. Each strip contains one well each of the mouse anti-alpha integrin monoclonal antibodies: (alpha1, alpha2, alpha3, alpha4, alpha5, alphaV, alphavbeta3), and one goat anti-mouse negative well. See the plate layout on product insert.
      • Cell Stain Solution: (Part No. 90144) One 20 mL bottle.
      • Extraction Buffer: (Part No. 90145) One 20 mL bottle.
      • Assay Buffer: (Part No. 90601) One 100 mL bottle.
      Detection methodChromogenic
      Applications
      ApplicationThis assay can substitute for FACS analysis & is useful for screening human adherent cells for cell surface integrins or for monitoring in vitro cell differentiation or genetic modification of cells.
      Application NotesThe CHEMICON® Alpha Integrin-Mediated Cell Adhesion Array Kit can be used for assessing the presence or absence of specific integrins on the cell surface. This assay can substitute for FACS analysis (3) and is useful for screening human adherent cells for cell surface integrins or for monitoring in vitro cell differentiation or genetic modification of cells.



      For Research Use Only. Not for use in diagnostic procedures.
      Biological Information
      Species Reactivity
      • Human
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes the alpha 1 subunit of integrin receptors. This protein heterodimerizes with the beta 1 subunit to form a cell-surface receptor for collagen and laminin. The heterodimeric receptor is involved in cell-cell adhesion and may play a role in inflammation and fibrosis. The alpha 1 subunit contains an inserted (I) von Willebrand factor type I domain which is thought to be involved in collagen binding.
      Gene Symbol
      • ITGA1
      • CD49a
      • VLA1
      • VLA-1
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P56199 # Integrin alpha-1/beta-1 is a receptor for laminin and collagen. It recognizes the proline-hydroxylated sequence G-F-P-G- E-R in collagen.
      SIZE: 1179 amino acids; 130848 Da
      SUBUNIT: Heterodimer of an alpha and a beta subunit. Alpha-1 associates with beta-1.
      SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.
      DOMAIN: SwissProt: P56199 The integrin I-domain (insert) is a VWFA domain. Integrins with I-domains do not undergo protease cleavage.
      SIMILARITY: Belongs to the integrin alpha chain family. & Contains 7 FG-GAP repeats. & Contains 1 VWFA domain.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsThe experimental and control plates can be stored at 2° to 8°C in the foil pouch up to their expiration. Unused strips may be placed back in the pouch for storage. Ensure that the desiccant remains in the pouch, and that the pouch is securely closed.

      Precautions

      · Cell stain contains minor amounts of crystal violet, a toxic substance, which may cause cancer and heritable genetic damage. Handle with caution. Toxic by inhalation and if swallowed. Irritating to eyes, respiratory system and skin.

      · Extraction buffer contains alcohol. Avoid internal consumption.
      Packaging Information
      Material Size1 plate
      Material Package96 wells
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      References

      Reference overviewPub Med ID
      Absence of ataxin-3 leads to cytoskeletal disorganization and increased cell death.
      Ana-João Rodrigues,Maria do Carmo Costa,Teresa-Luísa Silva,Daniela Ferreira,Fernanda Bajanca,Elsa Logarinho,Patrícia Maciel
      Biochimica et biophysica acta 1803 2010

      Show Abstract
      20637808 20637808

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      Technical Info

      Title
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      User Guides

      Title
      Alpha Integrin-mediated Cell Adhesion Array Kit

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      Categories

      Life Science Research > Cell Analysis > Cell-based Assays > Cell Adhesion Assays