Protein Purification
Amicon® Pro Purification System

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Amicon® Pro Purification System for protein purification. Reduce sample handling.
Amicon® Pro Application Note
American Laboratory posted a new application note using the Amicon® Pro system! Fast, Efficient Antibody Biotinylation for Cleaner Immunodetection Assays.

Read it here!
Traditional protein purification is a long process with many steps and multiple devices, often resulting in protein degradation and loss. Avoid the risks associated with sample transfer and reduce hands-on time when you bind, wash, elute and/or concentrate your protein in the all-in-one Amicon® Pro purification system. No matter what your workflow, the Amicon® Pro system delivers consistent, accurate sample preparation, resulting in more reliable recovery, uncompromised purity and easier data generation.

Webcast on Protein Purification
Listen to our latest on-demand webcast where you’ll learn how to improve the efficiency and yield of small-scale protein purification with a single centrifugal device.

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How Amicon® Pro Purification Works

The device combines affinity-based spin column purification with downstream sample concentration and buffer exchange. Featuring a large reservoir that accommodates a range of sample volumes, the device reduces the need for multiple centrifugation steps. Simply attach the included Amicon® Ultra filter for simultaneous elution, concentration and highly efficient diafiltration (>99%) in a single spin.

Amicon® Pro diagram

Amicon® Pro Purification System Applications

Not only does the Amicon® Pro purification system represent a game-changing improvement in general affinity purification, but it also transforms applications such as depletion of abundant proteins, protein enrichment, buffer exchange and clean-up of antibody labeling reactions. 

Because of the extremely flexible, modular design of the Amicon® Pro system, you can configure the perfect centrifugal purification device for your protein preparation.

Amicon® Pro Application
 
Protocol Steps
Maximum Capacity
Benefits3
Purification With buffer exchange and/or concentration
  • Bind
  • Clear and Wash
  • Elute/Concentrate
    +/- Buffer Exchange 
≤ 200 µL packed resin1
  • Speed
  • No sample transfer - No loss
  • Improved yield 
Purification only
  • Bind
  • Clear and Wash
  • Elute 
≤ 1000 µL packed resin2
  • Range of sample volumes can be processed
  • Single Elution - No fractions 
Diafiltration
  • Buffer Exchange +/- Concentrate 
Not Applicable
  • Speed
  • Improved activity of purified protein 
Antibody labeling
  • Buffer Exchange
  • Labeling Reaction
  • Wash and Concentrate
    +/- Buffer Exchange 
variable antibody input3
  • Speed
  • No sample transfer - No loss
  • Improved yield
  • Read application note 
Depletion or Enrichment
  • Bind
  • Deplete/Concentrate
  • +/- Wash/Concentrate
    +/- Buffer Exchange 
≤ 200 µL packed resin1
  • Speed
  • No sample transfer - No loss 

1 The assay capacity is dictated by the processing limitations of the Amicon® Ultra 0.5 mL device.
2 The Bind-Wash-Elute protocol is linearly scalable (50-1000 µL). Higher resin volumes require greater centrifugation speeds and duration to ensure filtrate clearance.
3 Benefits are relative to other current methods.