Protein Purification
Amicon Pro® Purification System

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Amicon Pro Purification System for protein purification. Reduce sample handling.
Amicon Pro Application Note
American Laboratory posted a new application note using the Amicon Pro system! Fast, Efficient Antibody Biotinylation for Cleaner Immunodetection Assays.
Read it here!
Traditional protein purification is a long process with many steps and multiple devices, often resulting in protein degradation and loss. Avoid the risks associated with sample transfer and reduce hands-on time when you bind, wash, elute and/or concentrate your protein in the all-in-one Amicon Pro purification system. No matter what your workflow, the Amicon Pro system delivers consistent, accurate sample preparation, resulting in more reliable recovery, uncompromised purity and easier data generation.xx

Webcast on Protein Purification
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How Amicon Pro Purification Works

The device combines affinity-based spin column purification with downstream sample concentration and buffer exchange. Featuring a large reservoir that accommodates a range of sample volumes, the device reduces the need for multiple centrifugation steps. Simply attach the included Amicon Ultra filter for simultaneous elution, concentration and highly efficient diafiltration (>99%) in a single spin.

Amicon Pro diagram

Amicon Pro Purification System Applications

Not only does the Amicon Pro purification system represent a game-changing improvement in general affinity purification, but it also transforms applications such as depletion of abundant proteins, protein enrichment, buffer exchange and clean-up of antibody labeling reactions. 

Because of the extremely flexible, modular design of the Amicon Pro system, you can configure the perfect centrifugal purification device for your protein preparation.

Amicon Pro Application
 
Protocol Steps
Maximum Capacity
Benefits3
Purification With buffer exchange and/or concentration
  • Bind
  • Clear and Wash
  • Elute/Concentrate
    +/- Buffer Exchange 
≤ 200 µL packed resin1
  • Speed
  • No sample transfer - No loss
  • Improved yield 
Purification only
  • Bind
  • Clear and Wash
  • Elute 
≤ 1000 µL packed resin2
  • Range of sample volumes can be processed
  • Single Elution - No fractions 
Diafiltration
  • Buffer Exchange +/- Concentrate 
Not Applicable
  • Speed
  • Improved activity of purified protein 
Antibody labeling
  • Buffer Exchange
  • Labeling Reaction
  • Wash and Concentrate
    +/- Buffer Exchange 
variable antibody input3
  • Speed
  • No sample transfer - No loss
  • Improved yield
  • Read application note 
Depletion or Enrichment
  • Bind
  • Deplete/Concentrate
  • +/- Wash/Concentrate
    +/- Buffer Exchange 
≤ 200 µL packed resin1
  • Speed
  • No sample transfer - No loss 

1 The assay capacity is dictated by the processing limitations of the Amicon Ultra 0.5 mL device.
2 The Bind-Wash-Elute protocol is linearly scalable (50-1000 µL). Higher resin volumes require greater centrifugation speeds and duration to ensure filtrate clearance.
3 Benefits are relative to other current methods.