MAPmates Single Plex Assays

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MILLIPLEX® MAP MAPmate™ Single Plex Biomarker Assays

Designing your own multiplex assay is easy with MILLIPLEX® MAP MAPmate™ assays. Plex up to 8 individual MAPmate™ assays together using our Cell Signaling Buffer and Detection Kit (48-602MAG) or include them in existing MILLIPLEX® MAP cell signaling kits to enhance the panel or serve as controls within the guidelines provided in the protocols.

Important MAPmate™ Guidelines

Consult the protocol prior to use.
  • All magnetic MAPmate™ assays require the Cell Signaling Buffer & Detection Kit (48-602MAG). This kit contains all necessary reagents except the MAPmate™ assay. Both a filter and flat-bottom plate are included for convenience.
    • MILLIPLEX® MAP Lysis Buffer (Cat.# 43-040)
    • MILLIPLEX® MAP Assay Buffer 2 (Cat.# 43-041)
    • MILLIPLEX® MAP Streptavidin-Phycoerythrin (Cat. # 45-001H)
    • MILLIPLEX® MAP Amplification buffer (Cat.# 43-024A)
    • MILLIPLEX® MAP HeLa Cell Lysate:Unstimulated (Cat. # 47-205)
    • 96-well plate and 2 sealers
    • 96-well filter plate and 2 sealers
Each MAPmate™ includes:
  • Capture Antibody (bead-conjugated)
  • Detection Antibody (biotin-conjugated)
  • Positive Control (stimulated cell lysate) for phosphorylated analytes which can be used to qualify assay performance or, with serial dilutions, to develop a standard curve

3 Easy Steps to Designing a Luminex® Multiplex Signaling Assay

Step 1
Choose the analytes you want to plex together and determine their compatibility. Keep in mind that the following cannot be plexed together:
  • Magnetic Bead MAPmates™ and non-Magnetic Bead MAPmates™
  • MAPmates™ that require a different assay buffer (see buffer table subtab)
  • Phosphospecific and total MAPmate™ pairs (e.g. total GSK3β and GSK3β (Ser9)
  • Pan Tyr and phospho-specific MAPmates™ (e.g. Phsopho-EGFR (pan-Tyr) and HSP27 (Ser78)
  • Phospho- MAPmates™ for a single target (e.g. Akt (Ser473) and Akt (Thr308))
  • GAPDH and β-Tubulin cannot be plexed with kits or MAPmates™ containing panTyr
Step 2
Order the MILLIPLEX® MAP Cell Signaling Buffer and Detection Kit (Cat. # 48-602MAG), required to run all MAPmate™ assays

Step 3
Order the MAPmate™ products you have selected.


Find and Purchase Your Favorite Analytes & Kits —
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MILLIPLEX® Analyte Kit Finder

Use the kit finder below to choose the correct assays for your biomarkers of interest, so you can obtain the best quality data the first time.
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Design & Purchase Your Own Kit

Already know your analytes of choice? Start designing and purchasing your own kits now!

Choose Customizable Panels & Premixed Kits – OR – Cell Signaling MAPmates™ Assays.

Design and price your MILLIPLEX® MAP Kits.