Related Resources: Brochures
| Application Notes
Download Your Copy of the New Analyte Quarterly
Find the perfect set of assays!
Get Your Copy
Presented by Science:
Characterizing the maternal immune environment during pregnancy: Implications for autism spectrum disorders
Speakers: Dr. Karen L. Jones, University of California-Davis, and Dr. Anthony Saporita, MilliporeSigma R&D
|Dr. Karen L. Jones
||Dr. Anthony Saporita
Looking for a little more information on autism and the immune system? Read the SelectScience article about Dr. Karen L. Jones’ research: Autism Linked to Abnormal Immune Responses of Pregnant Mothers: Learn how the maternal immune profile of mothers can predict neurodevelopmental abnormalities Read Article
Need Tips on Optimizing your Multiplex Assays?
Read the SelectScience Interview with Sonali Nayak, Bioscience Technical Services Manager, MilliporeSigma: Discover How Multiplex Assays are Advancing Biomedical Research: Top tips to optimize your analyses
Get Sonali Nayak’s take on how to best run your MILLIPLEX® MAP
assays. Read Article
As your research uncovers the significant role of more and more biomarkers in both normal and disease states, you need a reliable partner for quality assays and consistent performance. We not only offer you the largest portfolio of biomarker assays—including ELISAs, RIAs and MILLIPLEX® MAP
(multi-analyte profiling), our kits and reagents provide you with consistent, high-quality tools so that you can do your best work while saving time, labor and cost.
In addition to the assay specifications listed in the protocol, we evaluate other performance criteria during our validation process: cross-reactivity, dilution linearity, kit stability and sample behavior (e.g. detectability and stability).
Each MILLIPLEX® MAP
How to Decide Which MILLIPLEX® Assay Is Best for Your Research
In Method Comparison tests (comparing ELISA and MILLIPLEX® MAP
assays), while the absolute values are not exactly the same, the results do correlate. Hence when switching from one assay method to another, a correction factor may be used when comparing with past data. In most situations the dynamic range and the sensitivity of the assay may be better with the MILLIPLEX® kits.
Please contact Technical Support for more information of correlation factors.
To locate protocols and technical documents for a specific panel, choose any of these options:
Discover the Latest in New Multiplex and Single Plex Protein Detection — New Multiplex Kits!
384-Well Human High Sensitivity T Cell (21 plex) Assay
(Cat. No. HSTC384-28K
(Cat. No. HSTCMAG384-PX21
(Cat. No. HSTCMAG384PX21BK
Introducing kits for your FLEXMAP 3D® instrument: get more with 384
Now you can perform your human cytokine assays faster and more economically with the new MILLIPLEX® MAP Human High Sensitivity T-Cell 21-plex assay for 384-well plates.
Benefits of 384-well kits:
- Analytically validated to meet the same QC criteria as our 96-well kits.
- You get everything you need — all in one kit — including the confidence in your data that you’ve come to expect from MILLIPLEX® assays.
- One standard curve set for each plate
- Obtain up to 366 sample data points per plate (183 samples run in duplicate) in the same amount of time it would take you to run a 96-well plate
Convert existing kits to 384-well format
Our Custom Assay Development Services can make any existing MILLIPLEX® kit into a 384-well kit within a few weeks, including all species and kits. Just let us know what you need and when you need it, and we will develop a kit that is right for your laboratory.
View data showing comparable sensitivity between 384-well and 96-well assay kits.
|Analyte||96-well MinDC||384-well MinDC|
|A comparison of MinDCs between the 96-well MILLIPLEX MAP Human High Sensitivity T Cell Premix kit (Cat. No. HSTCMAG28SPMX21) and the 384-well MILLIPLEX MAP Human High Sensitivity T Cell Premix kit (Cat. No. HSTCMAG384-PX21).
Phospho/Total AKT 2-Plexes
The three Akt isoforms (Akt1, Akt2, and Akt3) may have unique, isoform-specific roles in differentiation, proliferation, and other processes. To measure relative levels of phospho- and total Akt1, Akt2, and Akt3, we developed MILLIPLEX® MAP Phospho/Total 2-plex assays for each of the Akt isoforms. Each 2-plex assay enables the measurement of both phosphorylated and total protein in the same well. In addition, each Phospho/Total 2-plex assay can be combined with any other (non-Akt) Phospho/Total 2 plex.
Data obtained using the Phospho/Total Akt Isoform 2-plex Assays are shown below for SH-SY5Y cells. To view MCF7 cell data, read the latest Analyte Quarterly brochure.
Data obtained using MILLIPLEX® MAP Cell Signaling Phospho/Total Akt isoform 2-plex assays with lysates of stimulated SH-SY5Y cells. MILLIPLEX® MAP Cell Signaling Phospho/Total Akt isoform 2-plex assays were pooled and used to evaluate the cellular response to retinoic acid (RA), which induces neuronal differentiation of SH-SY5Y cells. After 3 days, cells cultured in the presence or absence of RA were collected and lysed according to assay protocols. MFI measurements were obtained, and the ratios of phosphoprotein to total protein were calculated for each, Phospho/Total Akt1, Akt2 and Akt3 2 plexes.
Human Diabetes Premixed Panel
Use this new premixed panel to simultaneously quantify five analytes important in studies of diabetes and obesity: Insulin, Glucagon, Leptin, Glucagon-like peptide (GLP-1), and C-Peptide. This panel is a subset of our popular Human Metabolic Hormone Panel (HMHEMAG-34K) and exhibits high sensitivity, consistency, and reproducibility. Learn More about Human Diabetes Premixed Panel
Comparison of sensitivity, sample volume requirements, and standard curve range vs. electrochemiluminescence (ECL) detection platform
(MILLIPLEX® MAP) pg/mL
|Dynamic Range |
Human Liver Injury Panel
This panel is a five-plex assay kit to be used for the simultaneous detection of hepatotoxicity biomarkers, liver-type arginase 1 (ARG1), α-glutathione-S-transferase (GST-α), 5’nucleotidase (5’NT), sorbitol dehydrogenase (SDH), and malate dehydrogenase 1 (MDH1), in serum or plasma samples. These biomarkers are hepatocellular and hepatobiliary enzymes that are released into circulation upon occurrence of liver injury. The sensitivity of this multiplex assay panel is illustrated in the table below.Learn More about Human Liver Injury Panel
|Analyte||MinDC (pg/mL)||MinDC+2SD (pg/mL)|
Human RTK Phosphoprotein Panel
RTKs (Receptor Tyrosine Kinases) are transmembrane proteins that act as receptors for growth factors, neurotrophic factors and other extracellular signaling molecules. RTKs play important, interconnected roles in multiple biological pathways. Unlike traditional cell signaling assays, which are limited to measuring individual RTKs at a time, multiplex immunodetection enables you to screen multiple RTK-mediated signaling pathways at once, so you can quickly determine on which pathway to focus your study. Learn More about Human RTK Phosphoprotein Panel
Find and Price Your Favorite Analytes & Kits –
Two Tools to Make Ordering Faster and Easier!