Intracellular Pathway Assay Kits Using Luminex® Technology

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Measure activation of intracellular pathways, including cell signaling pathways and cellular metabolism, using MILLIPLEX® bead-based multiplex assays.
Multiplexing, using the Luminex xMAP® platform, provides faster answers to your intracellular pathway questions compared to traditional Western blots, mass spectrometry, and radioactive phosphorylation assays that require large amounts of sample.

Learn more about Luminex technology

MILLIPLEX® MAP intracellular pathway multiplex kits and panels are analytically validated using the same stringent performance criteria used to manufacture our circulating biomarker kits, and they are optimized to yield maximum data from your precious samples. Our cell signaling assays enable accurate relative quantitation of both total and phosphorylated forms of signaling proteins, revealing connections and crosstalk within your pathways of interest.

Bring your intracellular pathways to life with MILLIPLEX® MAP Multiplex Assays.

Rely on the quality we build into each kit to produce results you trust. In addition to the assay specifications listed in the protocol, we evaluate other performance criteria during our validation process: cross-reactivity, dilution linearity, kit stability, and sample behavior (e.g. detectability and stability).

Our analytically validated intracellular pathway assay kits provide:
  • Stimulated and unstimulated cell lysates provided to qualify assay performance. Browse cell lysates.
  • Premixed magnetic beads to capture analytes of interest 
  • Detection antibody cocktails designed to yield consistent analyte profiles within panel
Some examples of the intracellular pathway assay kits in our portfolio are listed below.

Cell Signaling Phosphoprotein + Total 2 Plex Assays

  • Directly compare the phosphoprotein vs. total protein levels in your sample by reading them in the same well
  • Combine with other phospho/total 2 plex assays to study phosphorylation and total expression of multiple proteins simultaneously
  • Get relatively quantitative fold-increase data from your multiplexed signaling assay kits
Use the Analyte Finder to browse all Cell Signaling Phosphoprotein + Total 2 Plex Assays.

Cell Signaling Phosphoprotein and Total Multiplex Premixed Assays

  • Measure multiple phosphoproteins or total proteins within or across pathways in a single sample
  • Get relatively quantitative fold increase data from your multiplexed signaling assay kits
Use the Analyte Finder to browse all Cell Signaling Phosphoprotein + Total Multiplex Premix Assays.

MAPmates™ Phosphoprotein and Total Single Plex Kits

  • Plex up to 8 individual MAPmates™ to design your own assay using our Cell Signaling Buffer and Detection Kit (Cat. No. 48-602MAG)*
  • Plex additional MAPmates™ into existing MILLIPLEX® MAP Cell Signaling kits*
  • Use β–tubulin or GAPDH as housekeeping/loading controls by plexing into an existing MILLIPLEX® MAP Cell Signaling kit*
  • Get relatively quantitative fold increase data from your multiplexed signaling assay kits
Learn more about MAPmate™ assays

To expand the power of your intracellular research, we also offer circulating MILLIPLEX® MAP multiplex assays for:
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Click here to learn more about how Luminex technology works.

Download the Analyte Quarterly brochure for a complete, updated list of assays.

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